Biocompatibility of modified poly-β-hydroxybutyric acid to adrenocortical cells

Abstract

Biodegradable polymers have been widely used in the field of tissue engineering. The adhesive characteristic of polymer surfaces must be modified for cell seeding and growth before being used. The modified method poly-β-hybroxybutyric acid (PHB) was introduced and the biocompatibility of modified PHB to adrenocortical cells was studied here, Gelatin was used as a modifier to retrofit PHB and adrenocortical cells were seeded in the modified PHB. The effects of PHB on the proliferation and secreting function of adrenocortical cells were evaluated by MTT and RIA methods. It shows that adrenocortical cells were able to grow and survived in PHB and no influence on the proliferation and secreting function of the cells was observed. The morphology study shows that the modified PHB was easy for cells seeding and growing. The modified PHB is a good biodegradable material as cell carrier for transplantation of adrenocortical cells.

Keywords

poly-β-hybroxybutyricacid / cell transplantation / biocompatibiltiy

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