Simultaneous determination of five components including acetaminophen by reversed-phase high performance liquid chromatography

Zhang Li-qing; Wu Xiao-hua; Lu Ying; Wang Xia

doi:10.1007/BF02838358

Journal of Wuhan University of Technology Materials Science Edition ›› 2004, Vol. 19 ›› Issue (1) : 34 -35. DOI: 10.1007/BF02838358

Article

Simultaneous determination of five components including acetaminophen by reversed-phase high performance liquid chromatography

Author information +

History +

PDF

Abstract

High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup, including acetaminophen, p-aminophenol, caffeine, chlorphenamine maleate and guaifenesin. The mobile phase consists of 15wt% acetonitrile, 0.004mol/L sodium heptyl sulfonate, 0.03 mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13:40:44:3), the pH of which is adjusted to 3.0 by phosphoric acid. The contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm, with a flow rate of 0.4 mL/min. The results show that the calibration curves are linear in a certain range. The average recovery of five components is between 96.31% and 102.3%.

Keywords

organic compounds / preparation and determination / chromatography

Cite this article

Download citation ▾

Zhang Li-qing, Wu Xiao-hua, Lu Ying, Wang Xia. Simultaneous determination of five components including acetaminophen by reversed-phase high performance liquid chromatography. Journal of Wuhan University of Technology Materials Science Edition, 2004, 19(1): 34-35 DOI:10.1007/BF02838358

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	Liqing Zhang, Xian-liang Tang Xi Zhu. The Simultaneous Determination of Five Domponents including Acetaminophen by Ridge Regression Spectrophotometry. Journal of Wuhan University of Technology—Mater. Sci. Ed, 2001, 16(2): 79-79.

[2]	Liqing Zhang, Xiaohua Wu, Xian-liang Tang Xi Zhu. Simultaneous Spectrophotometric Determination of Five-Components in Cough Syrup by Partial Least Squares. Journal of Analytical Science, 2002, 18(4): 318-318.

[3]	Liqing Zhang, Xiaohua Wu, Xian-liang Tang Xi Zhu. Research into Simultaneous Spectrophotometric Determination of Components in Cough Syrup by Principal Component Regression. Spectroscopy and Spectral Analysis, 2002, 22(3): 427-427.

[4]	Baty J D, Lindsay R M, Sheila Sharp. Use of High-performance Liquid Chromatography in the Measurement of in vitro Acetylation. J. Chromatogr, 1986, 353: 329-37.

[5]	Brega A, Prandini P, Amaglii, . Determination of phenol,m-, o- andp-cresol,p-aminophenol andp-nitrophenol in Urine by High-performance Liquid Chromatography. J Chromatogr, 1990, 535(1–2): 311-16.

[6]	Jain R, Bhatia A. Thin Layer Chromatographic Separation of Some Sulfa Drugs Using Acetylacetone as a Coupling Agent. J Indian Chem Soc, 1990, 67(7): 616-17.

[7]	Spell J C, Stewart J T. Quantitative Analysis ofp-aminosalicylic Acid and Its Major Impuritym-aminophenol inp-aminosalicylic. J Planar Chromatogr, 1994, 7(6): 472-3.