Action of selenium compounds on the cellular metabolism by microcalorimetry

Abstract

A new method is introduced to study the action between biomaterials and organism. By using an LKB-2277 bioactivity monitor and ampoule method, the fundamental thermogenesis curves of the metabolic process of pk-15 and the toxic effect of three kinds of selenomorpholine compounds on it were studied at 37°C. From the thermogenesis curves, the heat released by pk-15 metabolism was calculated. The results show that the selenium compounds all have toxic action on the metabolism process of pk-15 at the range of experimental concentrations. The sequence of the toxic action of selenium compounds is: Na₂SeO₃>β-(N-selenomorpholine)-ethyl phenylketone hydrochloride>selenomorpholine.

Keywords

liver mitochondria / metabolism / toxicity / microcalorimetry

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