Synthesis and purification of modified antisense oligodeoxyribonucleotides

Abstract

The synthesis and purification of modified antisense oligodeoxyribonucleotides (MAO) are desoribed. A new oligonucleotide purification method and a novel activating agent, dimethyl sulfoxide, for the condensation reaction are used. EDTA has been attached covalently to the 5′ end of the oligodeoxyribonucleotides sequence via an ethylenediamine linker in basic triethylammonium solution, then chelated with the metal ions such as Fe(II), Co(II) or Cu(II). High performance liquid chromatorgaphy is used to separate and purify this kind of MAO, using double mobile phase, triethylammonium acetate and acetonitrile. By using this routine chemical synthesis method, EDTA can be linked to the 5′ terminus of oligodeoxyribonucleotedes of any desired length and sequence, forming chimeric MAOs.

Keywords

oligodeoxyribonucleotedes / synthesis / purification / high performance liquid chromatography