Influence diversity of extracellular DNA on bioleaching chalcopyrite and pyrite by Sulfobacillus thermosulfidooxidans ST

Abstract

In this paper, Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite. The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the surface of chalcopyrite. The role of extracellular DNA (eDNA) in the bioleaching process was investigated by depletion of eDNA using DNase I. The number of cells attached on the chalcopyrite and pyrite surfaces decreased on a large scale, and the lag phase of cell growth increased, causing the leaching percentages of pyrite and chalcopyrite to decrease by approximately 11.6% and 20.5%, respectively. The formation and distribution of eDNA secreted during bioleaching was assessed by a fluorescent dye-based method and visualized by confocal laser scanning microscopy (CLSM). The content of eDNA increased with bioleaching time. Furthermore, ST showed a stronger capacity to produce eDNA on the surface of pyrite than on the surface of chalcopyrite. These results showed that the removal of eDNA has a more significant effect on the bioleaching of chalcopyrite than on pyrite.

References

[1] PengT-j, ZhouD, LiuY-n, YuR-I, QiuG-z, ZengW-ming. Effects of pH value on the expression of key iron/sulfur oxidation genes during bioleaching of chalcopyrite on thermophilic condition [J]. Annals of Microbiology, 2019, 69(6): 627-635

[2] ZhaoH-b, ZhangY-s, ZhangX, QianL, SunM-l, YangY, ZhangY-s, WangJ, KimH, QiuG-zhou. The dissolution and passivation mechanism of chalcopyrite in bioleaching: An overview [J]. Mineral Engineering, 2019, 136: 140-154

[3] ZengW-m, QiuG-z, ZhouH-b, LiuX-d, ChenM, ChaoW-l, ZhangC-g, PengJ-hua. Characterization of extracellular polymeric substances extracted during the bioleaching of chalcopyrite concentrate [J]. Hydrometallurgy, 2010, 96(3): 77-80

[4] MitsunobuS, ZhuM, TakeichiY, OhigashiT, TakahashiY. Direct detection of Fe(II) in extracellular polymeric substances (EPS) at the mineral-microbe interface in bacterial pyrite leaching [J]. Microbes and Environments, 2016, 31(1): 63-69

[5] GieseE C. Evidences of EPS-iron (III) ions interactions on bioleaching process mini-review: The key to improve performance [J]. Orbital the Electronic Journal of Chemistry, 2019, 11(3): 200-204

[6] VeraM, SchippersA, SandW. Progress in bioleaching: Fundamentals and mechanisms of bacterial metal sulfide oxidation-part A [J]. Applied Microbiology and Biotechnology, 2013, 97(17): 7529-7541

[7] GhauriM A, OkibeN, JohnsonD B. Attachment of acidophilic bacteria to solid surfaces: The significance of species and strain variations [J]. Hydrometallurgy, 2007, 85(2): 72-80

[8] VelmourouganeK, PrasannaR, SinghS B, KumarR, SahaS. Sequence of inoculation influences the nature of exopolymeric substances (EPS) and biofilm formation in Azotobacter chroococcum and Trichoderma viride [J]. FEMS Microbiology Ecology, 2017, 93(7): 1-13

[9] AiC-b, YanZ, ZhouH, HouS-s, ChaiL-y, QiuG-z, ZengW-min. Metagenomic insights into the effects of seasonal temperature variation on functional potentials of activated sludge [J]. Microorganisms, 2019, 713(7): 1-18

[10] DasT, KromB P, VanD M H C, BusscherH J, SharmaP K. DNA-mediated bacterial aggregation is dictated by acid-base interactions [J]. Soft Matter, 2011, 7(6): 2927-2935

[11] YuR-I, LiuA-j, LiuY-n, YuZ-J, PengT-j, WuX-l, ShenL, LiuY-d, LiJ-k, LiuX-d, QiuG-z, ChenM, ZengW-min. Evolution of Sulfobacillus thermosulfidooxidans secreting alginate during bioleaching of chalcopyrite concentrate [J]. Journal of Applied Microbiology, 2017, 122(6): 1586-1594

[12] DasT, SharmaP K, BusscherH J, MeiH C V D, KromB P. Role of extracellular DNA in initial bacterial adhesion and surface aggregation [J]. Applied & Environmental Microbiology, 2010, 76: 3405-3408

[13] BöckelmannU, JankeA, KuhnR, NeuT R, WeckeJ, LawrenceJ R, SzewzykU. Bacterial extracellular DNA forming a defined network-like structure [J]. FEMS Microbiology Letters, 2006, 262(1): 31-38

[14] WhitchurchC B, Tolker-NielsenT, RagasP C, MattickJ S. Extracellular DNA required for bacterial biofilm formation [J]. Science, 2002, 295(5559): 1487

[15] SteinbergerR E, HoldenP A. Extracellular DNA in single- and multiple-species unsaturated biofilms [J]. Applied & Environmental Microbiology, 2005, 7195404-5410

[16] VorkapicD, PresslerK, SchildS. Multifaceted roles of extracellular DNA in bacterial physiology [J]. Current Genetics, 2016, 62(1): 71-79

[17] CruzL F, CobineP A, FuenteL D L. Calcium increases Xylella fastidiosa surface attachment, biofilm formation, and twitching motility [J]. Applied & Environmental Microbiology, 2012, 78(5): 1321

[18] GeyikA G, CecenF. Variations in extracellular polymeric substances (EPS) during adaptation of activated sludges to new feeding conditions [J]. International Biodeterioration & Biodegradation, 2015, 105: 137-145

[19] OkshevskyM, MeyerR L. Evaluation of fluorescent stains for visualizing extracellular DNA in biofilms [J]. Journal of Microbiological Methods, 2014, 105102-104

[20] SenavélezM, RedondoC, GrahamJ H, CuberoJ. Presence of extracellular DNA during biofilm formation by Xanthomonas citri subsp. citri strains with different host range [J]. Plos One, 2016, 11(6): e156695

[21] PatelK K, SurekhaD B, TripathiM, AnjumM M, MuthuM S, TilakR, AgrawalA K, SinghS. Antibiofilm potential of silver sulfadiazine-loaded Nanoparticle formulations: A study on the effect of DNase-I on microbial biofilm and wound healing activity [J]. Molecular Pharmaceutics, 2019, 1693916-3925

[22] ÖzdemirC, AkcelukM, AkcelikN. The role of extracellular DNA in salmonella biofilms [J]. Molecular Genetics Microbiology and Virology, 2018, 33(1): 60-71

[23] RongX-m, HuangQ-y, ChenW-l, CaiP, LiangWei. Surface thermodynamical analysis of adsorption of bacteria on two soil clay minerals [J]. Acta Pedologica Sinica, 2010, 48(2): 331-337

[24] HuftonJ, HardingJ H, Romero-, GonzalezM E. The role of extracellular DNA in uranium precipitation and biomineralization [J]. Physical Chemistry Chemical Physics, 2016, 18(42): 29101-29112

[25] ZhangR-y, NeuT R, ZhangY-t, BellentS, KuhlicheU, LiQ, SandW, VeraM. Visualization and analysis of EPS glycoconjugates of the thermoacidophilic archaeonSulfolobus metallicus [J]. Applied Microbiology and Biotechnology, 2015, 99(17): 7343-7356

[26] YuR-I, HouC-w, LiuA-j, PengT-j, XiaM-c, WuX-l, ShenL, LiuY-d, LiJ-k, YangF, QiuG-z, ChenM, ZengW-min. Extracellular DNA enhances the adsorption of Sulfobacillus thermosulfldooxidans, strain ST on chalcopyrite surface [J]. Hydrometallurgy, 2018, 176: 97-103

[27] DingJ-n, GaoJ, WuX-l, ZhangC-g, WangD-z, QiuG-zhou. Jarosite-type precipitates mediated by YN22, Sulfobacillus thermosulfldooxidans, and their influences on strain [J]. Transactions of Nonferrous Metals Society of China, 2007, 17(5): 1038-1044

[28] GuoX, YinH-q, LiangY-l, HuQ, ZhouX-s, XiaoY-h, MaL-y, ZhangX, QiuG-z, LiuX-dong. Comparative genome analysis reveals metabolic versatility and environmental adaptations of Sulfobacillus thermosulfldooxidans Strain ST [J]. Plos One, 2014, 9(6): e99417

[29] ZengW-m, QiuG-z, ZhouH-b, PengJ-h, ChenM, TanS-n, ChaoW-l, LiuX-d, ZhangY-sheng. Community structure and dynamics of the free and attached microorganisms during moderately thermophilic bioleaching of chalcopyrite concentrate [J]. Bioresource Technol, 2010, 101187079-7086

[30] JiangB-xian. The Mensuration of the copper contents in the Stannum based alloy with BCO light-intensity method [J]. Heavy Castings & Forgings, 2006, 12(1): 38-39

[31] YeA-y, ZuoY-hu. Determination of iron in ion exchange resin by-phenanthroline [J]. Industrial Water Treatment, 2013, 33(3): 74-76

[32] SunC-z, MaiJ-h, WeiYan. Discussion on the Improment of the determination of S04²~ in water [J]. Trace Elements Science, 2001, 8(3): 55-56

[33] YuR-I, LiuZ-h, YuZ-j, WuX-l, ShenL, LiuY-d, LiJ-k, QinW-q, QiuG-z, ZengW-min. Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula [J]. International Journal of Minerals Metallurgy & Materials, 2019, 26(12): 1504-1511

[34] WuJ-f, XiC-wu. Evaluation of different methods for extracting extracellular DNA from the biofilm matrix [J]. Applied and Environmental Microbiology, 2009, 75(16): 5390-5395

[35] CorinaldesiC, DanovaroR, DellannoA. Simultaneous recovery of extracellular and intracellular DNA suitable for molecular studies from marine sediments [J]. Applied and Environmental Microbiology, 2005, 71(1): 46-50

[36] BoF, PalmgrenR, KeidingK, NielsenP H. Extraction of extracellular polymers from activated sludge using a cation exchange resin [J]. Water Research, 1996, 30(8): 1749-1958

[37] LiaoB-q, AllenD G, DroppoI G, LeppardG G, LissS N. Surface properties of sludge and their role in bioflocculation and settleability [J]. Water Research, 2001, 35(2): 339-350

[38] QinZ-q, OuY-z, YangL, ZhuY-l, Tolker-NielsenT, MolinS, QuDi. Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis [J]. Microbiology, 2007, 153(7): 2083

[39] Fuxman, BassJ I, RussoD M, GabelloniM L, GeffnerJ R, GiordanoM, CatalanoM, ZorreguietaA, TrevaniA S. Extracellular DNA: A major proinflammatory component of Pseudomonas aeruginosa biofilms [J]. Journal of Immunology, 2010, 184116386-6395

[40] ZrelliK, GalyO, LatourlambertP, KirwanL, GhigoJ M, BeloinC, HenryN. Bacterial biofilm mechanical properties persist upon antibiotic treatment and survive cell death [J]. New Journal of Physics, 2013, 15(12): 5026

[41] RanjithaV R, MuddegowdaU, RavishankarR V. Potent activity of bioconjugated peptide and selenium nanoparticles against colorectal adenocarcinoma cells [J]. Drug Development and Industrial Pharmacy, 2019, 4591-21

[42] JiaC-y, WeiD-z, LiuW-g, HanC, GaoS-l, WangY-juan. Selective adsorption of bacteria on sulfide minerals surface [J]. Transactions of Nonferrous Metals Society of China, 2008, 18(5): 1247-1252

[43] LoosdrechtM C V, LyklemaJ, NordeW, SchraaG, ZehnderA J. Electrophoretic mobility and hydrophobicity as a measured to predict the initial steps of bacterial adhesion [J]. Applied & Environmental Microbiology, 1987, 53(8): 1898-1901

[44] GehrkeT, HallmannR, KinzlerK, SandW. The EPS of Acidithiobacillus ferrooxidans-a model for structure-function relationships of attached bacteria and their physiology [J]. Water Science and Technology, 2001, 43(6): 159-167

[45] RodriguezY, BallesterA, BlazquezM L, GonzalezF, MuñozJ A. Study of bacterial attachment during the bioleaching of pyrite, chalcopyrite, and sphalerite [J]. Geomicrobiology Journal, 2003, 20(2): 131-141

[46] HuangT, LiD-wei. Presentation on mechanisms and applications of chalcopyrite and pyrite bioleaching in biohydrometallurgy—A presentation [J]. Biotechnology Reports, 2014, 4(1): 107-119

[47] HeZ-g, YangY-p, ZhouS, HuY-h, ZhongHui. Effect of pyrite, elemental sulfur and ferrous ions on EPS production by metal sulfide bioleaching microbes [J]. Transactions of Nonferrous Metals Society of China, 2014, 24(4): 1171-1178

[48] YingX, LiuY-a, ZhaoLiang. Study on the function of the extracellular DNA in the Candida albicans biofilm formation in the root canal [J]. Journal of Modern Stomatology, 2009, 84: 1-17

[49] DesaiS, SanghrajkaK, GajjarD. High adhesion and increased cell death contribute to strong biofilm formation in Klebsiellapneumoniae [J]. Pathogens (Basel, Switzerland), 2019, 8(4): 1504-1511

[50] HarneitK, SandW. Influence of growth substrate and attachment substratum on EPS and biofilm formation by Acidithiobacillus ferrooxidans [J]. Advanced Materials Research, 2007, 20–21385

[51] YuR-I, LiuJ, TanJ-x, ZengW-m, ShiL-j, GuG-h, QinW-q, QiuG-zhou. Effect of pH values on the extracellular polysaccharide secreted by Acidithiobacillus ferrooxidans during chalcopyrite bioleaching [J]. International Journal of Minerals Metallurgy and Materials, 2014, 21(4): 311-316

[52] ZengW-m, TanS-n, ChenM, QiuG-zhou. Detection and analysis of attached microorganisms on the mineral surface during bioleaching of pure chalcopyrite with moderate thermophiles [J]. Hydrometallurgy, 2011, 106(1): 46-50

[53] SchippersA, SandW. Bacterial leaching of metal sulfides proceeds by two indirect mechanisms via Thiosulfate or via Polysulfides and sulfur [J]. Applied and Environmental Microbiology, 1999, 65(1): 319-321

[54] AiC-b, YanZ, ChaiH-s, GuT-y, WangJ-j, ChaiL-y, QiuG-z, ZengW-min. Increased chalcopyrite bioleaching capabilities of extremely thermoacidophilic Metallosphaera sedula inocula by mixotrophic propagation [J]. Journal of Industrial Microbiology & Biotechnology, 2019, 46(8): 1113-1127