Effect of inoculation during different phases of agricultural waste composting on spectroscopic characteristics of humic acid

Hong-li Huang , Guang-ming Zeng , Lin Luo , Jia-chao Zhang , Man Yu , Pu-feng Qin

Journal of Central South University ›› 2015, Vol. 22 ›› Issue (11) : 4177 -4183.

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Journal of Central South University ›› 2015, Vol. 22 ›› Issue (11) : 4177 -4183. DOI: 10.1007/s11771-015-2965-0
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Effect of inoculation during different phases of agricultural waste composting on spectroscopic characteristics of humic acid

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Abstract

The white-rot fungus, Phanerochaete chrysosporium (P. chrysosporium), was inoculated during different phases of agricultural waste composting and its effect on the spectroscopic characterization of humic acid (HA) was studied. Three runs were used in this study: Run A was the control without inoculating, and Runs B and C were inoculated P. chrysosporium during the first and the second fermentation phase, respectively. The elemental analysis, ultra-violet spectroscopy (UV), fluorescence spectra, Fourier transform infra-red (FTIR) and 13C nuclear magnetic resonance (13C-NMR) of HA all lead to the same conclusion, that is, the degree of aromatization and polymerization of HA increases after 42 days composting. However, the inoculation during different phases presents different effects. P. chrysosporium increases the degree of aromatization and polymerization of HA when it is inoculated during the second fermentation phase, while it does not produce an obvious change on the humification degree of HA when it is inoculated during the first fermentation phase.

Keywords

inoculation / composting / Phanerochaete chrysosporium / humic acid / spectroscopic characteristics / agricultural waste

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Hong-li Huang, Guang-ming Zeng, Lin Luo, Jia-chao Zhang, Man Yu, Pu-feng Qin. Effect of inoculation during different phases of agricultural waste composting on spectroscopic characteristics of humic acid. Journal of Central South University, 2015, 22(11): 4177-4183 DOI:10.1007/s11771-015-2965-0

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