Enzymatic detection of ethanol based on H₂O₂-sensitive quantum dots

Abstract

A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H₂O₂. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AO_x) which, on oxidation of ethanol, produces H₂O₂. The method has higher sensitivity, wider analytical range (0.1–8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.

Keywords

enzymatic detection / CdSe/ZnS quantum dots / alcohol oxidase / hydrogen peroxide / photoluminescence

References

[1] TangermanA.. Highly sensitive gas chromatographic analysis of ethanol in whole blood, serum, urine, and fecal supernatants by the direct injection method [J]. Clinical Chemistry, 1997, 43(6): 1003-1009

[2] YaritaT., NakajimaR., OtsukaS., IharaT., TakatsuA., ShibukawaM.. Determination of ethanol in alcoholic beverages by high performance liquid chromatography-flame ionization detection using pure water as mobile phase [J]. Journal of Chromatography A, 2002, 976(1/2): 387-391

[3] HarmomB. J., PattersonD. H., RegnierF. E.. A electrophoretically mediated microanalysis of ethanol [J]. Journal of Chromatography A, 1993, 657: 429-434

[4] WenG.-m., ZhangY., ShuangS.-m., DongC., ChoiM. M. F.. Application of a biosensor for monitoring of ethanol [J]. Biosensors and Bioelectronics, 2007, 23(1): 121-129

[5] LuoP., LiuY., XieG.-m., XiongX.-l., DengS.-x., SongF.-zhou.. Determination of serum alcohol using a disposable biosensor [J]. Forensic Science International, 2008, 179(2): 192-198

[6] LeeC. A., TsaiY. C.. Preparation of multiwalled carbon nanotube-chitosan-alcohol dehydrogenase nanobiocomposite for amperometric detection of ethanol [J]. Sensors and Actuators B: Chemical, 2009, 138(2): 518-523

[7] BarsanM. M., BrettC. M. A.. An alcohol oxidase biosensor using PNR redox mediator at carbon film electrodes [J]. Talanta, 2008, 74(5): 1505-1510

[8] GoriushkinaT. B., SoldatkinA. P., DzydeyychS. V.. Application of amperometric biosensors for analysis of ethanol, glucose, and lactate in wine [J]. Journal of Agricultural and Food Chemistry, 2009, 57(15): 6528-6535

[9] WuaL.-n., LeiJ.-p., ZhangX.-j., JuH.-xian.. Biofunctional nanocomposite of carbon nanofiber with water-soluble porphyrin for highly sensitive ethanol biosensing [J]. Biosensors and Bioelectronics, 2008, 24(4): 644-649

[10] SimonD. N., CzolkR., AcheH. J.. Doped sol-gel films for the development of optochemical ethanol sensors [J]. Thin Solid Films, 1995, 260(1): 107-110

[11] JiaT.-t., CaiZ.-m., ChenX.-m., LinZ.-j., HuangX.-l., ChenX., ChenG.-nan.. Electrogenerated chemiluminescence ethanol biosensor based on alcohol dehydrogenase functionalized Ru(bpy)3 2+ doped silica nanoparticles [J]. Biosensors and Bioelectronics, 2009, 25(1): 263-267

[12] ZhangL.-h., XuZ.-a., SunX.-p., DongS.-jun.. A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)3 2+-Au nanoparticles aggregates [J]. Biosensors and Bioelectronics, 2007, 22(6): 1097-1100

[13] ChanW. C. W., NieS.. Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J]. Science, 1998, 281(5385): 2016-2018

[14] ZhongP., YuY., WuJ.-z., LongZ.-y., LiangC.-sui.. Synthesis of mercaptoethylaminecoated CdSe/CdS nanocrystals and their use for DNA probe [J]. Analytical Sciences, 2007, 23(9): 1085-1089

[15] ZhangB.-h., QiL., WuF.-ying.. Functionalized m anganese-doped zinc sulfide core/shell quantum dots as selective fluorescent chemodosimeters for silver ion [J]. Microchimica Acta, 2010, 170(1/2): 147-153

[16] WangJ., LiangJ.-g., ShengZ.-h., HanH.-you.. A novel strategy for selective detection of Ag⁺ based on the red-shift of emission wavelength of quantum dots [J]. Microchimica Acta, 2009, 167(3/4): 281-287

[17] FuX., HuangK.-l., LiuS.-qin.. A rapid and universal bacteria-counting approach using CdSe/ZnS/SiO₂ composite nanoparticles as fluorescence probe [J]. Analytical and Bioanalytical chemistry, 2010, 396(4): 1397-1404

[18] SneeP. T., SomersR. C., NairG., ZimmerJ. P., BawendiM. G., NoceraD. G.. A ratiometric CdSe/ZnS nanocrystal pH sensor [J]. Journal of the American Chemical Society, 2006, 128(41): 13320-13321

[19] AliE. M., ZhengY.-g., YuH. H., YingJ. Y.. Heavy metal ion detection by glutathione-capped quantum dots [J]. Analytical Chemistry, 2007, 79(24): 9452-9458

[20] YuanJ.-p., GuoW.-w., YangX.-r., WangE.-kang.. Anticancer drug-DNA interactions measured using a photoinduced electron-transfer mechanism based on luminescent quantum dots [J]. Analytical Chemistry, 2009, 81(1): 362-368

[21] BoenemanK., MeiB. C., DennisA. M., BaoG., DeschampsJ. R., MattoussiH., MedintzI. L.. Sensing Caspase 3 activity with quantum dot-fluorescent protein assemblies [J]. Journal of the American Chemical Society, 2009, 131(11): 3828-3829

[22] ShiangY.-c., HuangC.-c., ChangH.-tsung.. Gold nanodot-based luminescent sensor for the detection of hydrogen peroxide and glucose [J]. Chemical Communications, 2009, 14(23): 3437-3439

[23] CaoL.-h., YeJ., TongL.-l., TangBo.. A new route to the considerable enhancement of glucose oxidase (GOx) activity: The simple assembly of a complex from CdTe quantum dots and GOx, and its glucose sensing [J]. Chemistry-A European Journal, 2008, 14(31): 9633-9640

[24] HuM., TianJ., LuH.-t., WengL.-x., WangL.-hui.. H₂O₂-sensitive quantum dots for the label-free detection of glucose [J]. Talanta, 2010, 82(3): 997-1002

[25] BadeaM., CurulliA., PalleschiG.. Oxidase enzyme immobilisation through electropolymerised films to assemble biosensors for batch and flow injection analysis [J]. Biosensors and Bioelectronics, 2003, 18(5/6): 689-698

[26] SalgadoA. M., FollyR. O. M., ValdmanB., CosO., ValeroF.. Colorimetric method for the determination of ethanol by flow injection analysis [J]. Biotechnology Letters, 2000, 22(4): 327-330