Production of microbial oils co-fermented by Trichoderma.koningii and Trichosporon cutaneum with Daqu distiller’s grains

Abstract

Daqu distiller’s grains were co-fermented as raw materials by Trichoderma.koningii and Trichosporon cutaneum to produce microbial oils, which can provide raw materials for bio-diesel development. The single factor tests were used to investigate the effects of ratio of strains, inoculum size, culture temperature and culture time on the production of microbial oils. The best processing conditions were obtained by orthogonal test through measuring the content of microbial oils in product. The result shows that the microbial oils content of the co-fermented product under the ratio of Trichoderma.koningii to Trichosporon cutaneum of 1:1, the inoculum size of strains of 11% at 28 °C for 6 d is 7.15 g/L. It is shown that the production of microbial oils co-fermented by Trichoderma.koningii and Trichosporon cutaneum with Daqu distiller’s grains is possible. The research provides a new idea for the reuse of Daqu distiller’s grains and also provides a new way for the development of microbial oils.

Keywords

Daqu distiller’s grains / Trichoderma.koningii / Trichosporon cutaneum / co-fermentation / microbial oils

References

[1] ZhuJ.-l., ZhangG.-jie.. Current situation and development trend of the research and production of biodiesel [J]. Chemical Industry Times, 2004, 18(1): 23-27

[2] LiX.-s., LuB.-s., JiangH.-rong.. Production of microbial oils fermented by two-strains with corn stalk [C]. 2010 3rd International Conference on Future BioMedical Information Engineering, 2010, Qiqihaer, Institute of Elestrical Electronics Engineers, Inc.: 322-325

[3] LiuX.-h., ChenM., LiuD., LiLing.. Study on starter-making techniques by waste distiller s grains instead of partial wheat [J]. Liquor-making Science amd Technology, 2003, 117(3): 85

[4] YuY.-g., SunC.-g., XiongX., YangZ.-l., LuoJun.. New technology applied in fermentation of refermentative dregs [J]. The Food Industry, 2005, 4: 31-33

[5] ZhaoD., PengZ.-y., NiuG.-j., ChenL., YangRong.. Recycle and utilization of distiller’s grains for Chinese liquor production by fortified fermentation [J]. China Brewing, 2011, 227(2): 147-149

[6] LiX.-s., LuB.-s., WuC.-lan.. Application of enzyme in the refermentation with Daqu Distiller’s grain [J]. Biotechnology, 2008, 18(1): 76-78

[7] LiX.-s., LuB.-s., LiX.-wu.. Study on Geotrichum candidium link cultured by Daqu Distiller’s grains to produce se-enriched feed protein [J]. Liquor-Making Science & Technology, 2007, 23(8): 144-145

[8] LiX.-s., LuB.-s., TangFeng.. Study on the application of compound enzymes and Geotrichum candidum link in co-fermentation to produce feed protein with distiller’s grain [J]. Feed Industry, 2007, 28(22): 12-13

[9] LiX.-s., HeH.-m., LuB.-s., WuLiang.. Study on feedstuff poduction by cellulase and multi-strains co-fermentation with distiller’s grains of Daqu as raw materials [J]. Liquor-Making Science & Technology, 2008, 171(9): 99-100

[10] NiuG.-j., LiuJ., SunD.-wei.. Study on the production of single cell protein with liquor lees [J]. Liquor Making, 2010, 37(2): 28-30

[11] LuB.-s., LiX.-s., WangF.-y., ZhangFei.. Study on the cultivation of sauce’s seed culture medium with Daqu distiller’s grain [J]. Liquor Making, 2007, 34(2): 41-43

[12] LuB.-s., LiX.-she.. Studying on the cultivation of sauces seed culture with daqu distiller s grain [J]. China Condiment, 2006, 12: 21-23

[13] LuB.-s., LiX.-she.. Effect of the production of bran vinegar using Daqu distiller’s grain as partly substitute for rice bran [J]. China Condiment, 2007, 1(1): 45-47

[14] JiangH., PanS.-l., ZhangL., ZhangS.-y., HuCheng.. Study on manufacturing activated carbon from discarded distiller s grains [J]. Food and Fermentation Industries, 2006, 32(6): 77-80

[15] WangH.-y., WangT.-f., WangR.-ming.. Study on the production of organic fertilizer by waste distiller’s grains [J]. Liquor-Making Science & Technology, 2007, 158(8): 142-143

[16] LiX.-s., LuB.-s., ZengH.-bo.. Application of Trichosporon cutaneum in the production of microbial oil by enzymatic hydrolysis of distiller’s grains [J]. Liquor-Making Science & Technology, 2009, 186(12): 87-90

[17] HuangX.-l., XiaL.-qiu.Microbiology experimental guide [M], 2006, Beijing, Higher Education Press: 1-7

[18] WangZ.-xu.. The production and utilization of microbial oils [J]. Journal of Cereals & Oils, 2005, 28(10): 13-14