Electrochemical mechanism of rusticyanin (Rus.) isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode

Abstract

Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode. The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction. The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19–43 °C. The reduction ability of the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8. Fe²⁺ ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials (φ_p) markedly move negatively.

Keywords

rusticyanin / acidithiobacillus ferrooxidans / cyclic voltammetry / electrochemistry