Preparation of new tissue engineering bone-CPC/PLGA composite and its application in animal bone defects

Abstract

To investigate the feasibility of implanting the biocomposite of calcium phosphate cement (CPC)/polylactic acid-polyglycolic acid (PLGA) into animals for bone defects repairing, the biocomposite of CPC/PLGA was prepared and its setting time, compressive strength, elastic modulus, pH values, phase composition of the samples, degradability and biocompatibility in vitro were tested. The above-mentioned composite implanted with bone marrow stromal cells was used to repair defects of the radius in rabbits. Osteogenesis was histomorphologically observed by using an electron-microscope. The results show that compared with the CPC, the physical and chemical properties of CPC/PLGA composite have some differences in which CPC/PLGA composite has better biological properties. The CPC/PLGA composite combined with seed cells is superior to the control in terms of the amount of new bones formed after CPC/PLGA composite is implanted into the rabbits, as well as the speed of repairing bone defects. The results suggest that the constructed CPC/PLGA composite basically meets the requirements of tissue engineering scaffold materials and that the CPC/PLGA composite implanted with bone marrow stromal cells may be a new artificial bone material for repairing bone defects because it can promote the growth of bone tissues.

Keywords

biomaterials / mesenchymal stem cells (MSCs) / calcium phosphate cement (CPC) / polylactic acid-polyglycolic acid (PLGA) / bone defects

References

[1] CraneG. M., IshaugS. L., MikosA. G.. Bone tissue engineering [J]. Nat Med, 1995, 1(12): 1322-1324

[2] ZhengL., WangQ., PeiG.-xian.. Selection on extracellular matrix materials in bone tissue engineering [J]. Chinese Journal of Surgery, 2000, 38(10): 745-748

[3] BaiB., YeM.-chang.. The current situation and future of extracellular matrix materials in bone tissue engineering [J]. Progress in Modern Biomedicine, 2007, 7(1): 138-141

[4] ZhangX.-f., ZhangZ.-hong.. Research development of mineralized biomaterials with osteoinductivity [J]. Progress in Modern Biomedicine, 2007, 7(5): 777-779

[5] DriessensF. C. M., BoltongM. G., BermudezO., PlanellJ. A.. Formulation and setting times of some calcium orthophosphate cements: A pilot study [J]. J Mater Sci: Mater in Med, 1993, 4(5): 503-508

[6] BaadsgaardK.. Defect pseudoarthroses: An experimental study in rabbits [J]. Acta Orthop Scand, 1969, 40(6): 689-695

[7] ZellinG., LindeA.. Treatment of segmental defects in long bones using osteopromotive membranes and recombinant human bone morphogenetic protein-2: An experimental study in rabbits [J]. Scand J Plast Reconstr Surg Hand Surg, 1997, 31(2): 97-104

[8] FeiZ.-q., HuY.-y., WuD.-c., WuH., LuR., BaiJ.-p., SongH.-xun.. Preparation and property of a novel bone graft composite consisting of rhBMP-2 loaded PLGA microspheres and calcium. Phosphate cement [J]. Journal of Materials Science: Material in Medicine, 2007, 19(3): 1109-1116

[9] LiQ.-hong.. Progress in research of repairing large segmental bone defects of long bones in China [J]. Chinese Journal of Orthopaedics, 1997, 17: 13-14

[10] LshikawaK., AsaokaK.. Estimation of ideal mechanical strength and critical porosity of calcium phosphate cement [J]. J Biomed Mater Res, 1995, 29(12): 1537-1543

[11] GinebraM. P., FernandezE., BoltongM. G., BermudezO., PlanellJ. A., DriessensF. C. M.. Compliance of an apatitic calcium phosphate cement with the short-term clinical requirements in bone surgery, orthopaedics and dentistry [J]. Clin Mater, 1994, 17(2): 99-104

[12] XuJ., XiongG.-r., JiangPing.. Relationships between stress distribution of mandible with age and incidence of fracture [J]. Journal of Jiangsu University: Medicine Edition, 2006, 16(4): 291-293

[13] ZhangWei.The preparation of, and experimental study on compound bioactive artificial bone (CBAB) [D], 2001, Shanghai, The Second Military Medical University

[14] MuruganR., RamakrishnaS.. Nano-featured scaffolds for tissue engineering: A review of spinning methodologles [J]. Tissue Engineering, 2006, 12(3): 435-447

[15] XiangQ.-j., LiuY., ZhengZ., SuZ.-a., WangJ.-l., XiaoFei.. Preparation and some property investigations on composite of calcium phosphate cements/polyglycolic-polylactic acid [J]. Materials Science and Engineering of Powder Metallurgy, 2005, 10(6): 361-364