Effect of substrate concentration on stability of anammox biofilm reactors

Abstract

Ammonium and nitrite are two substrates of anammox bacteria, but they are also inhibitors under high concentrations. The performance of two anaerobic ammonium-oxidizing (anammox) upflow biofilm (UBF) reactors was investigated. The results show that anammox UBFs become unstable under nitrogen loading rate (NLR) applied higher than 1.0 g/(L·d). The consumptions of acidity in the anammox reaction lead to the increase of pH, which is as high as 8.70–9.05. Free nitrous acid concentration is accompanied to be lower than the affinity constant of anammox bacteria, and then starvation effect appears. Moreover, free ammonia concentration increases to 57–178 mg/L, resulting in inhibitory effect on the anammox bacteria. Both negative effects contribute to the instability of the anammox bioreactors.

Keywords

anammox biofilm bioreactor / pH / free ammonia / free nitrous acid / nitrogen removal

References

[1] MulderA., van de GraafA., RobertsonL., KuenenJ.. Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor [J]. FEMS Microbiol Ecol, 1995, 16(3): 177-184

[2] van de GraafA., de BruijnP., RobertsonL., JettenM., KuenenJ.. Autotrophic growth of anaerobic ammonium-oxidizing microorganisms in a fluidized bed reactor [J]. Microbiology (UK), 1996, 142(8): 2187-2196

[3] van DongenU., JettenM., van LoosdrechtM.. The SHARON®-Anammox® process for treatment of ammonium rich wastewater [J]. Water Sci Technol, 2001, 44(1): 153-160

[4] van der StarW., AbamaW., BolmmersD., MulderJ., TokutomiT., StrousM., PicioreanuC., van LoosdrechtM.. Startup of reactors for anoxic ammonium oxidation: Experiences from the first full-scale anammox reactor in Rotterdam [J]. Water Res, 2007, 41(18): 4149-4163

[5] ZhangH., ZhouS.. Treating leachate mixture with anaerobic ammonium oxidation technology [J]. J Cent South Univ Technol, 2006, 13(6): 663-667

[6] StrousM., HeijnenJ., KuenenJ., JettenM.. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms [J]. Appl Microbiol Biotechnol, 1998, 50(5): 589-596

[7] Dapena-MoraA., CamposJ., Mosquera-CorralA., JettenM., MendezR.. Stability of the anammox process in a gas-lift reactor and a SBR [J]. J Biotechnol, 2004, 110(2): 159-170

[8] JinR., ZhengP., HuA., MahmoodQ., HuB., JilaniG.. Performance comparison of two anammox reactors: SBR and UBF [J]. Chem Eng J, 2008, 138(1/3): 224-230

[9] SossaK., AlarocnM., AspeE., UrrutiaH.. Effect of ammonia on the methanogenic activity of methylaminotrophic methane producing archaea enriched biofilm [J]. Anaerobe, 2004, 10(1): 13-18

[10] KadamP., BooneD.. Influence of pH on ammonia accumulation and toxicity in halophilic, methylotrophic methanogens [J]. Appl Environ Microbiol, 1996, 62(12): 4486-4492

[11] van HulleS.Modelling, simulation and optimization of autotrophic nitrogen removal processes [D], 2005, Ghent, Ghent University

[12] GanigueR., LopezH., BalaguerM., ColprimJ.. Partial ammonium oxidation to nitrite of high ammonium content urban landfill leachates [J]. Water Res, 2007, 41(15): 3317-3326

[13] VadiveluV., KellerJ., YuanZ.. Effect of free ammonia and free nitrous acid concentration on the anabolic and catabolic processes of an enriched Nitrosomonas culture [J]. Biotechnol Bioeng, 2006, 95(5): 830-839

[14] Apha-Awwa-WefStandard methods for water and wastewater examination [M], 199820th edWashington DC, American Public Health Association: 258-284

[15] ChamchoiN., NitisoravutS.. Anammox enrichment from different conventional sludges [J]. Chemosphere, 2007, 66(11): 2225-2232

[16] LiuS., YangF., GongZ., MengF., ChenH., XueY., FurukawaK.. Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal [J]. Bioresour Technol, 2008, 99(15): 6817-6825

[17] ZhongH.-c., ZhouS.-q., YaoJ.-q., ZhuM.-s., HuY.-c., WuF.. Comparative study of experimental start-up and operation in two UASB-ANAMMOX reactors [J]. Journal of Chemical Industry and Engineering, 2007, 58(11): 2798-2804

[18] StrousM., KuenenJ., JettenM.. Key physiological parameters of anaerobic ammonium oxidation [J]. Appl Environ Microbiol, 1999, 65(7): 3248-3250

[19] WardR., AshleyC.. Comparative effects of ammonia and related compounds on Poliovirus [J]. Appl Environ Microbiol, 1978, 36(1): 198-200

[20] Mosquera-corralA., GonzalezF., CamposJ., MendezR.. Partial nitrification in a SHARON reactor in the presence of salt and organic carbon compounds [J]. Process Biochem, 2005, 40(9): 3109-3118

[21] JinR.-C.Performance of autotrophic nitrogen removal bioreactors [D], 2007, Hangzhou, Zhejiang University

[22] ZhengP., HuB.-lan.. Kinetics of anaerobic ammonium oxidation [J]. Chinese Journal of Biotechnology, 2001, 17(2): 193-198

[23] Dapena-MoraA., FernandezI., CamposJ., Mosquera-CorralA., MendezR., JettenM.. Evaluation of activity and inhibition effects on anammox process by batch tests based on the nitrogen gas production [J]. Enzyme Microb Technol, 2007, 40(4): 859-865

[24] StrousM.Microbiology of anaerobic ammonium oxidation [D], 2000, Delft, TU Delft

[25] WakiM., TokutomiT., YokoyamaH., TanakaY.. Nitrogen removal from animal waste treatment water by Anammox enrichment [J]. Bioresour Technol, 2007, 98(14): 2775-2780