Bioleaching of soluble phosphorus from rock phosphate containing pyrite with DES-induced Acidithiobacillus ferrooxidans

Abstract

The effects of Acidithiobacillus ferrooxidans (At. f) mutated with diethyl sulfate (DES) as a mutagen on the bioleaching of soluble phosphorus (P) from rock phosphate (RP) were investigated. The results show that the oxidative activity of At. f is greatly improved by 1.0% (volume fraction) of DES. Correspondingly, the highest leaching rate of soluble P is also obtained to be 14.9% by the At. f mutated, which is 85.8% higher than that of the adapted At. f without mutation. In addition, the SEM images are significantly performed that the corrosion of RP residue surfaces leached by 1.0% DES-induced At.f is much worse than that of leached by the adapted At. f. All the above indicate that the leaching efficiency of soluble P from RP with pyrite can be greatly improved by using DES-induced At. f to a certain extent.

Keywords

bioleaching / rock phosphate (RP) / Acidithiobacillus ferrooxidans (At. f) / diethyl sulfate / soluble phosphorus / mutation

References

[1] HaymanD. S.. Phosphorus cycling by soil microorganisms and plant roots [J]. Soil Microbiology, 1975, 5: 67-97

[2] HildaR., ReynaldoF.. Phosphate solubilizing bacteria and their role in plant growth promotion [J]. Biotechnology Advances, 1999, 17: 319-339

[3] NautiyalC. S., BhadauriaS., KumarP., LalH., MondalR., VermaD.. Stress induced phosphate solubilization in bacteria isolated from alkaline soils [J]. FEMS Microbiol Lett, 2000, 182: 291-296

[4] KhasawnshF. E., DollE. C.. The use of phosphate rock for direct application to soils [J]. Advances in Agronomy, 1978, 30: 159-205

[5] GleisnerM., HerbertR. B., KockumP. C. F.. Pyrite oxidation by Acidithiobacillus ferrooxidans at various concentrations of dissolved oxygen [J]. Chemical Geology, 2006, 225(1/2): 16-29

[6] YaoY.-j., ZhangY.-g., LaiQ.-ke.. Research progress of leaching mechanism of sulphide minerals with T. f [J]. Hydrometallurgy of China, 2004, 23(3): 122-127

[7] ShiS.-y., FangZ.-heng.. Bioleaching of marmatite flotation concentrate by Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans [J]. Transactions of Nonferrous Metals Society of China, 2004, 14(3): 569-575

[8] HosselniT. R., KolahdoozanM., TabatabaeiY. S. M.. Bioflotation of Sarcheshmeh copper ore using Thiobacillus Ferrooxidans bacterium [J]. Minerals Engineering, 2005, 18(3): 371-374

[9] ChiR.-a., XiaoC.-q., GaoH., WuY.-x., LiS.-r., WangC.-wen.. Biodecomposition of low-grade rock phosphate with some bacterium and fungi [J]. The Chinese Journal of Process Engineering, 2005, 5(6): 636-639

[10] ChiR.-a., XiaoC.-q., GaoH., WuY.-x., LiS.-r., WangC.-w., YuF.-quan.. Study on solubilization of rock phosphate by some microbes [J]. Industrial Minerals and Processing, 2005, 34(7): 4-7

[11] ChiR.-a., XiaoC.-q., GaoH.. Bioleaching of phosphorus from rock phosphate containing pyrite by Acidithiobacillus ferrooxidans [J]. Minerals Engineering, 2006, 19(9): 979-981

[12] GongW.-q., ChenW., ZhangX.-z., BianX., LiuY.-j., LiuJ., HuangY.-b., YangH.-gang.. Isolation and cultivation of Acidithiobacillus ferrooxidans and its effects on bioleaching of phosphate ore [J]. The Chinese Journal of Process Engineering, 2007, 7(3): 54-58

[13] XiaL.-x., ZengJ., DingJ.-l., YangY., ZhangB., LiuJ.-s., QiuG.-zhou.. Comparison of three induced mutation methods for Acidiothiobacillus caldus in processing sphalerite [J]. Minerals Engineering, 2007, 20(14): 1323-1326

[14] ReyesI., BaziramakeengaR., BernierL., AntounH.. Solubilization of phosphate rocks and minerals by a wild-type strain and two UV-induced mutants of Penicillium rugulosum [J]. Soil Biology and Biochemistry, 2001, 33: 1741-1747

[15] VarenyuamA., SavantV. V., ReddyS. M.. Phosphate solubilization by a wild type strain and UV-induced mutants of Aspergillus tubingensis [J]. Soil Biology and Biochemistry, 2007, 39: 695-699

[16] XIONG Ying, HU Jian-ping, LIN Bin-lan, ZHENG Cun-jiang. Study on the domestication and mutagenic selection of Thiobacillus ferrooxidans [J]. Multipurpose Utilization of Mineral Resources, 2001(6): 27–31. (in Chinese)

[17] JIANG Jing-long, JIA Jian-bo. Study on Breeding of Thiobacillus ferrooxidans mutated with NTG [J]. Multipurpose Utilization of Mineral Resources, 2005(1): 21–24. (in Chinese)

[18] XuX.-j., MengY.-s., GongL., JiangJ.-e.. Bacterium T. f mutated by UV and bio-leaching of low grade Chalcopyrite Ores [J]. Mining and Metallurgical Engineering, 2005, 25(1): 34-36

[19] ChiR.-a., XiaoC.-q., HuangX.-h., WangC.-w., WuY.-xin.. A novel approach to bioleach soluble Phosphorus from rock phosphate [J]. The Chinese Journal of Process Engineering, 2007, 7(2): 310-316

[20] ZHANG Zai-hai, WANG Dian-zuo, QIU Guan-zhou, HU Yue-hua. Discussion of mutation breeding for ferrous oxidation of Thiobacillus ferrooxians [J]. Copper Engineering, 2001(1): 12–15. (in Chinese)

[21] GaoL., DengN.-s., YaoJ.-long.. Determination of iron in manganese ore by imagemetry [J]. Chemistry and Bioengineering, 2006, 23(6): 61-62

[22] LazaroffN., SigalW., WassermanA.. Iron oxidation and precipitation of ferric hydroxysulfates by resting Thiobacillus ferrooxidans cells [J]. Applied and Environmental Microbiology, 1982, 43: 924-938

[23] JiangL.-h., ZhaoS.-l., ZhuJ.-hua.. Fast destination of P2O5 in melamine phosphate [J]. Chemical Engineer, 2001, 85(4): 50-51