Isolation, identification and characterization of cadmium-resistant Pseudomonas aeruginosa strain E₁

Abstract

Strain E₁ with resistance to 18 mmol/L cadmium (Cd), isolated from Cd-contaminated soil was identified by morphological observation, biochemical and physiological characterization and 16S rDNA sequence analysis. The resistance to heavy metals Cd, Cu, Co, Mn, Pb, Zn and 12 antibiotics was examined. The ability of removing Cd from solution was studied. The characterizations show that strain E₁ is affiliated to Pseudomonas aeruginosa (P. aeruginosa). Strain E₁ has high resistance to heavy metals and the order is found to be Cd>Mn>Zn>Cu>Pb>Co in solid media. Strain E₁ also exhibits the resistance to 12 antibiotics. Both living and non-living cells of strain E₁ can remove Cd from solution, and living cell has better biosorption than non-living cell.

Keywords

identification / P. aeruginosa / cadmium-resistance / screening

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