Isolation and characterization of YNTC-1, a novel Alicyclobacillus sendaiensis strain

Abstract

A heterotrophic acidothermophilic bacterial strain, YNTC-1, was isolated from an acidic hot spring in Tengchong, Yunan, China. YNTC-1 grows at pH value of 1.5–8.0 and temperature of 40–70 °C, with optimal pH and temperature at 3.0 and 55 °C, respectively. The cells of the strain are in shape of short rod, with 1.0–1.2 μm in length and 0.7–0.8 μm in diameter, and with distinct spores at both poles of each cell. The predominant fatty acids in cellular membrane of the strain are C18:1 θ7c. 16s rRNA gene analysis reveals that this strain is closely related to Alicyclobacillus sendaiensis, with over 99% sequence similarity. Based on phenotypic and genotypic analyses, YNTC-1 is identified as a member of A. sendaiensis. Considering some important morphological and biochemical differences between strain YNTC-1 and A. sendaiensis ATCC 27009T, YNTC-1 may be proposed to be a novel subspecies of A. sendaiensis. However, this viewpoint has to be confirmed by further studies. Co-bioleaching of pyrite and chalcopyrite with strain YN22, Sulfobacillus thermosulfidooxidans, shows that strain YNTC-1 has no evident influence on bioleaching rates of these two sulphide minerals.

Keywords

bioleaching / sulphide minerals / isolation / strain YNTC-1 / Alicyclobacillus sendaiensis / Sulfobacillus thermosulfidooxidans

References

[1] WitneJ. Y., PhillipsC. V.. Bioleaching of Ok Tedi copper concentrate in oxygen-and carbon dioxide-enriched air [J]. Minerals Engineering, 2001, 14(1): 25-48

[2] KrebsW., BrombacherC., BosshardP. P., BachofenR., BrandlH.. Microbial recovery of metals from solids [J]. FEMS Microbiol Rev, 1997, 20(3/4): 605-617

[3] BoseckerK.. Bioleaching: Metal solubilization by microorganisms [J]. FEMS Microbiol Rev, 1997, 20(3/4): 591-604

[4] RohwerderT., GehrkeT., KinzlerK., SandW.. Bioleaching review (part A): Progress in bioleaching: Fundamentals and mechanisms of bacterial metal sulfide oxidation [J]. Appl Microbiol Biotechnol, 2003, 63(3): 239-248

[5] JohnsonD. B.. Biodiversity and ecology of acidophilic microorganisms [J]. FEMS Microbiol Ecol, 1998, 27(4): 307-317

[6] JohnsonD. B.. Importance of microbial ecology in the development of new mineral technologies [J]. Hydrometallurgy, 2001, 59(2/3): 147-157

[7] RodgersL. E., HoldenP. J., FosterL. J. R.. Culture of Acidiphilium cryptum BV1 with halotolerant Alicyclobacillus-like spp.: Effects on cell growth and iron oxidation [J]. Biotechnology Letters, 2002, 24(18): 1519-1524

[8] XiaJ.-l., PengA.-a., HeHuan.. A new strain Acidithiobacillus albertensis BY-05 for bioleaching of metal sulfides ores [J]. Trans Nonferrous Met Soc China, 2007, 17(1): 168-175

[9] GotoK., MochidaK., AsaharaM., SuzukiM., KasaiH., YokotaA.. Alicyclobacillus pomorum sp. nov.: A novel thermoacidophilic, endospore-forming bacterium that does not possess ω-alicyclic fatty acids, and emended description of the genus Alicyclobacillus [J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(5): 1537-1544

[10] WuY.-p., XuJ.-m., WangH.-zhen.. application of Sherlockmis in identification of soil bacteria [j]. Acta Pedologica Sinica, 2006, 43(4): 642-647

[11] GotoK., MatsubaraH., MochidaK., MatsumuraT., HaraY., NmaM., YamasatoK.. Alicyclobacillus herbarius sp. nov.: A novel bacterium containing ω-cycloheptane fatty acids, isolated from herbal tea [J]. International Journal of Systematic and Evolutionary Microbiology, 2002, 52(1): 109-113

[12] NaokiT., YuriI., OsamuS., HisashiH., ToruN., TokuzoN.. Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan [J]. International Journal of Systematic and Evolutionary Microbiology, 2003, 53(4): 1081-1084

[13] ChenS.-q., ChenW.-f., HuX.-s., ZhaoG.-hua.. Rapid identification of Alicyclobacillus isolated from concentrated apple juice processing by 16S rDNA PCR-RFLP [J]. Journal of Chinese Institute of Food Science and Technology, 2006, 6(2): 99-102

[14] SimbahanJ., DrijberR., BlumP.. Alicyclobacillus vulcanalis sp. nov., a thermophilic, acidophilic bacterium isolated from Coso Hot Springs, California, USA [J]. International Journal of Systematic and Evolutionary Microbiology, 2004, 54(5): 1703-1707

[15] ChenS.-q., TangQ.-y., ZhangX.-d., ZhaoG.-h., HuX.-s., LiaoX.-j., ChenF., WuJ.-h., XiangHua.. Isolation and characterization of hermoacidophilic endospore-forming bacteria from the concentrated apple juice-processing environment [J]. Food Microbiology, 2006, 23(5): 439-445

[16] ChenZ.-w., JiangC.-y., LiuS.-jiang.. Survey on and phylogeny of Alicyclobacillus species in hot springs of southern China’s Guangdong and Yunnan Provinces [J]. Microbiology, 2004, 31(3): 50-54

[17] ShuR.-b., RuanR.-m., WenJ.-kan.. Review on passivation of chalcopyrite during bioleaching process [J]. Chinese Journal of Rare Metal, 2006, 30(3): 395-400

[18] YasuhiroK., MasahikoT., SatoruA.. Copper recovery from chalcopyrite concentrate by acidophilic thermophile Acidianus brierleyi in batch and continuous-flow stirred tank reactors [J]. Hydrometallurgy, 2001, 59(2/3): 271-282

[19] FalcoL., PoglianiC., CurutchetG.. A comparison of bioleaching of covellite using pure cultures of Acidithiobacillus ferrooxidans and acidithiobacillus thiooxidans or a mixed culture of Leptospirillum ferrooxidans and Acidithiobacillus thiooxidans [J]. Hydrometallurgy, 2003, 71(1/2): 31-36

[20] NaokoO., JohnsonD. B.. Biooxidation of pyrite by defined mixed cultures of moderately thermophilic acidophiles in pH-controlled bioreactors: Significance of microbial interactions [J]. Biotechnology and Bioengineering, 2004, 87(5): 574-583