Fast preparation of photopolymerized monolithic columns for capillary electrochromatography

Abstract

Photopolymerized sol-gel(PSG) columns were prepared using methacryloxypropyltrimethoxysilane as the monomer, toluene as the porogen and hydrochloric acid as the catalyst. Four different photoinitiators such as benzoin methyl ether, Irgacure 819, Irgacure 1700 and Irgacure 1800 were comparatively used in the reaction solution in the presence and absence of sodium dodecyl sulfate. The above eight solutions were respectively irradiated at 365 nm for 5–10 min in each capillary (75 μm inside diameter) to prepare the porous monolithic sol-gel column by a one-step, in situ, process. The chromatographic behavior of the eight PSG columns were comparatively studied, all of which exhibit reversed-phase character. Using these columns, several neutral compounds, namely thiourea, benzene, toluene, ethyl benzene, biphenyl and naphthalene can be separated from mixtures with a largest column efficiency of 74 470 plate/column for thiourea. Addition of sodium dodecyl sulfate in the polymerization process has a significant influence on the morphology and migration time.

Keywords

electrochromatography / monolithic columns / photopolymerized sol-gel / aromatic hydrocarbons

References

[1] ZhangY.-p., GongW.-j., FanL.-q., LeeK. P.. Novel method of preparation of monolithic capillary columns for capillary electrochromatography by γ-ray irradiation[J]. Chin Chem Lett, 2006, 17: 1485-1488

[2] GongW.-j., ZhangY.-j., ZhangY.-ping.. Rapid preparation of monolithic columns for capillary electrochromatography separation[J]. Chin Chem Lett, 2006, 17: 813-815

[3] ZhongF.-j., ChenX.-q., ZhangS.-c., LiY.-ping.. Organic acids and inorgranic anions in Bayer liquors by ion chromatography after solid-phase extraction[J]. Journal of Central South University of Technology, 2007, 14(1): 191-195

[4] PreinerstorferB., LindnerW., LammerhoferM.. Polymethacrylate-type monoliths functionalized with chiral amino phosphonic acid-derived strong cation exchange moieties for enantioselective nonaqueous capillary electrochromatography and investigation of the chemical composition of the monolithic polymer[J]. Electrophoresis, 2005, 26: 2005-2018

[5] MotokawaM., OhiraM., MinakuchiH.. Performance of octadecylsilylated monolithic silica capillary columns of 530 μm inner diameter in HPLC[J]. J Sep Sci, 2006, 29: 2471-2477

[6] HenchL. L., WestJ. K.. The sol-gel process[J]. Chem Rev, 1990, 90: 33-72

[7] JancoM., XieS., PetersonD. S., DulayM. T., QuirinoJ. P., ZareR. N.. Effect of porosity and surface chemistry on the characterization of synthetic polymers by HPLC using porous polymer monolithic columns[J]. J Sep Sci, 2002, 25: 909-916

[8] GatschelhoferC., MagnesaC., PieberT. R., BuchmeiserbM. R., SinneraF. M.. Evaluation of ring-opening metathesis polymerization (ROMP)-derived supports[J]. J Chromatogr A, 2005, 1090: 81-89

[9] DulayM. T., QuirinoJ. P., ZareR. N.. Bonded-phase photopolymerized sol-gel monoliths for reversed phase capillary electrochromatography[J]. J Sep Sci, 2002, 25: 3-9

[10] KatoM., Sakai-katoK., Toyo’okaT., DulayM. T., QuirinoJ. P., BennettB. D., ZareR. N.. Effect of prepartatory conditions on the performance of photopolymerized sol-gel monoliths for capillary electrochromatography [J]. J Chromatogr A, 2002, 961: 45-51

[11] DulayM. T., QuirinoJ. P., BennettB. D., ZareR. N., KatoM., ZareR. N.. Photopolymerized sol-gel monoliths for capillary electrochromatography[J]. Anal Chem, 2001, 73(16): 3921-3926

[12] KatoM., DulayM. T., BennettB. D., ZareR. N.. Photopolymerized sol-gel frits for packed column in capillary electrochromatography[J]. J Chromatogr A, 2001, 924: 187-195

[13] MorishimaK., BennettB. D., DulayM. T., ZareR. N.. Toward sol-gel electrochromatographic separations on a chip[J]. J Sep Sci, 2002, 25: 1226-1230

[14] Sakai-katoK., KatoM., Toyo’okaT.. On-line trypsin-encapsulated enzyme reactor by the sol-gel method integrated into capillary electrophoresis[J]. Anal Chem, 2002, 74(13): 2943-2949

[15] Sakai-katoK., KatoM., Toyo’okaT.. On-line drug-metabolism system using microsomes encapsulated in a capillary by the sol-gel method and integrated into capillary electrophoresis[J]. Anal Biochem, 2002, 38: 278-284

[16] ShaoH., DengQ.-l., LunZ.-h., YanC., GaoR.-yu.. Preparation and evaluation of monolithic silica columns for capillary electrochromatography[J]. Chin J Chromatogra, 2005, 23: 243-246