In vitro dynamic adsorption properties of sulfated glucomannan gel beads

Abstract

Sulfated konjac glucomannan gel beads(KGMSB) were prepared, its dynamic adsorption abilities for blood low-density lipoprotein and very low density lipoprotein cholesterol(LDL+VLDL-C) and the effect on hemorheology were studied. Konjac glucomannan(KGM) was used as raw material, after cross-linked by epichlorohybrin solution, and was then prepared into gel beads, hardened, and sulfated, final product KGMSB was obtained. The structure of KGMSB was observed by SEM. SEM analysis shows that KGMSB possesses cross-linked porous network structure. In vitro dynamic adsorption indicates that the adsorption rate for total cholesterol(TC), low-density and very low-density lipoprotein cholesterol(LDL+VLDL-C), high-density lipoprotein cholesterol(HDL-C) of KGMSB are (62.10±7.96)%, (63.42±8.27)%, (40.25±5.73)% (n=6), respectively. The ratio of HDL-C/LDL-C is increased by (30.12±7.07)%. The hemorheology studies show that whole blood viscosity and plasma viscosity are changed significantly (p<0.05). KGMSB can selectively adsorb LDL+VLDL-C from the whole blood, it can also reduce the blood viscosity and modulate hemorheology properties. KGMSB is a potential material to be developed into a novel blood LDL adsorbent.

Keywords

konjac glucomannan / sulfated / low-density lipoprotein / dynamic adsorption