Microwave-assisted extraction of polysaccharides from solanum nigrum

Abstract

The microwave-assisted method was used to extract polysaccharides from solanum nigrum. The optimum experimental parameters, mechanism of the extraction and the effect of microwave-assisted extraction process on the structures of polysaccharides were investigated. The extract was analyzed by the modified phenol-sulfuric acid method at 490 nm. The optimum experimental parameters were obtained by orthogonal experiments as follows: extraction time 15 min, microwave radiation power 455 W and the process ratio of materials mass to solvent volume 1:20. The results show that compared with the conventional reflux extraction, the microwave-assisted extraction has a higher yield in shorter time, with no effect on the finally obtained polysaccharides as seen from the FT-IR spectra. The scanning electron microscopy images reveal that the mechanism of the extraction is related to the structural changes of the plant cells in different extracting conditions.

Keywords

solanum nigrum / polysaccharides / structure / microwave-assisted extraction

References

[1] LuCui-xia, LiXiu-rong. The general study of clinic and experiment on the treatment on hepatic cellular carcinoma in middle or advanced stage with Chinese heat-clearing and detoxifying herb [J]. Journal of Shandong University of Traditional Chinese Medicine, 1999, 23(3): 224-227(in Chinese)

[2] DingGuo-dong, YangGuang-wen, ZhangXiao-jian. The evaluation on the clinical efficacy of the treatment on hepatic carcinoma in middle or advanced stage with Dongshen injection[J]. Study and Clinical Application on Tumor, 1999, 11(2): 123-124(in Chinese)

[3] XiaoGui-wu, ZengHe-pin. Isolation, purification and identification of polysaccharides from black night-shade(solanum nigrum) (II) [J]. Chinese Traditional and Herbal Drugs, 2000, 31(3): 162-164(in Chinese)

[4] XiaoGui-wu, Shen-Lan, ZengHe-pin. The analysis of the water-soluble polysaccharides from solanum nigrum L[J]. Chinese Medicinal Materials, 1999, 22(8): 406-406(in Chinese)

[5] XiaoGui-wu, ZengHe-pin. Isolation, purification and identification of polysaccharides from solanum nigrum L [J]. Pharmaceutical Biotechnology, 1998, 5(3): 157-160(in Chinese)

[6] MoHai-hong, ZengHe-pin, XiaoGui-wu. Study on water-soluble products from Solanum nigrum L. [J]. Journal of South China Normal University, 1995, 18(2): 41-42(in Chinese)

[7] KaufmannB, ChristenP. Recent extraction techniques for natural products: microwave assisted extraction and pressurized solvent extraction [J]. Phytochemical Analysis, 2002, 13: 105-113

[8] Ganzler K. A novel sample preparation method for chromatography[J]. Journal of Chromatography, 1986 (371): 299.

[9] BrachetA, ChristenP, VeutheyJ. Focused Microwave-assisted extraction of cocaine and benzoylecgonine from coca leaves [J]. Phytochemical Analysis, 2002, 13: 162-169

[10] BeatriceK, PhilippeC. Parameters affecting microwave-assisted extraction of alkaloids[J]. Phytochemical Analysis, 2001, 12: 327-331

[11] MarcV, RoserR. Determination of arsenic species in oyster tissue by microwave-assisted extraction and liquid chromatography-atomic fluorescence detection [J]. Applied Organometallic Chemistry, 2001, 15: 658-666

[12] LauraC, NadjezdaH. Ultrasonication and microwave assisted extraction of degradation products from degradable polyolefin blends aged in soil[J]. Journal of Applied Polymer Science, 2001, 79: 1101-1112

[13] Pare J R J, Signomin M, Lapointe J. Microwave-assisted natural products extraction[P]. US 5002784, 1991.

[14] Pare J R J, Gloucesters J. Microwave extraction of volatile oil and apparatus[P]. Europe 0485668 A1, 1992.

[15] ChenSau Soon, SpiroMichael. Kinetics of microwave extraction of rosemary leaves in hexane, ethanol and a hexane + ethanol mixture. Flavour and Fragrance Journal, 1995, 10(2): 101-112