Growth kinetics of Thiobacillus ferrooxidans in bioelectrochemical cell

Abstract

Thiobacillus ferrooxidans might be the most important bacteria used in biometallurgy. The foundation way of its growth process is oxidizing ferrous in order to obtain energy needed for metabolism, but the variation of ferrous concentration and mixed potential of the culture media would have crucial effect on the bacteria growth. Based on the characteristics of Thiobacillus ferroxidans growth and redox potential of ferric and ferrous, an electrochemical cell was designed conventionally to study growth rule and the relationship between redox potential and bacteria growth was built up, and some growth kinetics of Thiobacillus ferrooxidans were elucidated. It demonstrates that the variation of open potential of electrochemical cell ΔE shows the growth tendency of Thiobacillus ferrooxidans, at the initial growth stage, the value of δE increases slowly, when at logistic growth stage, it increases drastically, and the growth rate of bacteria is linear with the oxidation rate of ferrous. The bacteria growth kinetics model is proposed using Monod and Michealis-Menten equation, and the kinetics parameters are got. The consistence of the measured and the calculated results proves that it is proper to use the proposed kinetics model and the electrochemical cell method to describe the growth rule of Thiobacillus ferrooxidans.

Keywords

Thiobacillus ferrooxidans / electrochemical cell / bioleaching / kinetics

References

[1] LiHong-xu, WangDian-zuo, HuYue-hua, et al.. Electrochemistry on oxidation of Fe²⁺ on Thiobacillus ferrooxidans modified powder microelectrode[J]. The Chinese Journal of Nonferrous Metals, 2002, 12(6): 1263-1267(in Chinese)

[2] LiHong-xu, WangDian-zuo, QiuGuan-zhou. The influence of Cu²⁺ to the oxidation of Fe²⁺ on the Thiobacillus ferrooxidans modified powder microelectrode [J]. The Chinese Journal of Nonferrous Metals, 2003, 13(1): 229-233(in Chinese)

[3] ZhangZai-hai, QiuGuan-zhou, HuYue-hua, et al.. Uv-induced mutagenesis of oxidation activity of ferrous ion of Thiobacillus ferrooxidans [J]. Trans Nonferrous Met Soc China, 2001, 11(5): 795-799

[4] SampsonM I, PhilipsC V, BallA S. Investigation of the attachment of Thiobacillus ferrooxidation to minearls using scanning electron microscopy analysis[J]. Minerals Engineering, 2000, 13: 643-56

[5] RowsonN, SaeidA. Bacterial leaching—beneficial bugs [J]. Mine and Quarry, 1997, 26: 35-40

[6] Natarajan K A. Electrochemical aspect of bioleaching multisulfide minerals[J]. Minerals and Metallurgical Processing, 1988, (50):61–65.

[7] LangD S, LawsonF. Kinetics of the liquid-phase oxidation of acid ferrous sulfate by the bacterium Thiobacillus ferrooxidans [J]. Biotechnology and Bioengineering, 1970, 12: 29-50

[8] EcclestonM, KellyD P. Oxidation kinetics and chemostat growth kinetics of Thiobacillus ferrooxidans on tetrathionate and thiosulfate [J]. J Bacteriol, 1978, 134: 718-727

[9] DispiritoA A, TuovineO H. Kinetics of uranous and ferrous iron oxidation by Thiobacillus ferrooxidans [J]. Arch Microbiol, 1982, 133: 33-37

[10] BraddockJ F, LuongH V, BrownE J. Growth kinetics of Thiobacillus ferrooxidans isolated from arsentic mine drainage [J]. Applied and Environment Microbiology, 1984, 48: 48-55

[11] KaramanevD G, NikolovL N. Influence of physicochemical parameters on bacterial activity of biofilm: ferrous iron oxidation by Thiobacillus ferrooxidans [J]. Biotechnology and Bioengineering, 1988, 31: 295-299

[12] NagpalS A. Structured model for Thiobacillus ferrooxidans growth on ferrous iron[J]. Biotechnology and Bioengineering, 1997, 53: 310-319

[13] PesicB, OilverD. An electrochemical method of measuring the oxidation rate of ferrous to ferric iron with oxygen in the presence of Thibacillus ferrooxidans[J]. Biotechnology and Bioengineering, 1989, 33: 428-439

[14] YunkerS B, RodovichJ M. Enhancement of growth and ferrous iron oxidation rate of T. ferrooxidans by electrochemical reduction of ferric iron[J]. Biotechnology and Bioengineering, 1986, 28: 1867-1875

[15] BlakeR C, HowardG T, McGinnessS. Enhanced yields of iron-oxidizing bacteria by in situ-electrochemical reduction of soluble iron in the growth medium[J]. Appl Environ Microbiol, 1994, 60: 2704-2710