Simultaneous determination of geniposidic acid, chlorogenic acid and geniposide in eucommia by HPLC

Abstract

A high performance liquid chromatography (HPLC) method was established for simultaneous determination of geniposidic acid, chlorogenic acid and geniposide in eucommia. Detection at 240 nm with a reversed-phase column, CH₃OH volume fraction, acidic additive and pH value of mobile phase were studied for their effects on the separability of the compounds. The most suitable separation was obtained with isocratic gradient elution system using CH₃OH-H₂O-H₃PO₄ (12.00 : 87.96 : 0.04, volume ratio) at a flow-rate of 1.0 mL/min. Under the experimental conditions, the capacity factors of three compounds are in 3–13. The sample is separated rightly. The analysis time is 30 min and the retention time of geniposidic acid, chlorogenic acid and geniposide are 6.7 min, 10.5 min and 21 min, respectively.

Keywords

eucommia / HPLC / geniposidic acid / chlorogenic acid / geniposide

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