Extraction process of chlorogenic acid in flos lonicerae by enzymatic treatment

Abstract

A new method of extracting chlorogenic acid from flos lonicerae, and treating the materials with enzyme before being extracted by ethanol is developed, and the optimum conditions are also investigated in detail. Three important factors, enzyme dosage, treatment time and treatment temperature are adapted to optimize the extraction process. The experimental results show that the extract yield of flos lonicerae and chlorogenic acid can be obviously increased by the cellulase treatment, 61.5 mg chlorogenic acid is obtained from 1.00 g flos lonicerae at most. The optimal temperature of enzymatic treatment is 40–50 °C. Compared with the use of single cellulase, the combined treatment of cellulase and pectinase increase the extract yield obviously but fail to improve that of chlorogenic acid.

Keywords

flos lonicerae / chlorogenic acid / enzymatic treatment / extract process

References

[1] MaXi-han, WeiQin, JingQian-ping, et al.. A comparison on the extraction of chlorogenic acids with different concentrations of ethanol from honeysuckle flower[J]. Journal of Northwest Forestry University, 2000, 15(4): 74-81(in Chinese)

[2] LiuZhen-li, ZhangQiu-hai, OuXing-chang, et al.. Effects of ultrafiltration and alcohol precipitation on chlorogenic acid in flos lonicerae[J]. Chinese Traditional Patent Medicine, 1996, 18(2): 4-5(in Chinese)

[3] GaoYu-min, WangMing-zhou, WangJian-ping, et al.. Study on the chemical constituents of honeysuckle flower[J]. Journal of Chinese herbal medicine, 1995, 26(11): 568-569(in Chinese)

[4] LiuXiang-lan, LiuChong-fang, ZhangYing, et al.. Study on different extraction process of chlorogenic acid in flos lonicerae and optimum[J]. Chinese Traditional Patent Medicine, 2000, 22(6): 402-405(in Chinese)

[5] LuizC, TrugoS, RobertM, et al.. Chlorogenic acid composition of instant coffees [J]. Analyst, 1984, 109(4): 263-266

[6] BrolisM, GabettaB, FuzzatiN, et al.. Identification by high-performance liquid chromatography-diode array detection-mass spectrometry and quantification by high-performance liquid chromatography-UV absorbance detection of active constituents of hypericum perforatum [J]. J Chromatogr A, 1998, 825: 9-16