A brief review of photoactive materials for photoelectrochemical biosensors

Abstract

Photoelectrochemical (PEC) biosensors have drawn growing interest due to their capability to detect biomolecules with the help of generating photocurrent during oxidation reactions, followed by their high sensitivity, minimal background interference, cost-efficiency, and portability. This review provides an extensive summary of the photoactive materials that power PEC biosensor performance. We start by outlining the basic ideas and signal-generating processes of PEC biosensing, highlighting the crucial role of charge-carrier dynamics in photocurrent production. The article's main body thoroughly examines several categories of photoactive materials, such as metal oxides, quantum dots, organic materials, plasmonic nanostructures, and two-dimensional nanomaterials. We go over the special qualities, charge-transfer methods, light-harvesting capacities, and effects on biosensor performance of each material type, all supported by current experimental research. To improve sensitivity and selectivity, we also examine key design techniques, including heterojunction formation, surface functionalization, and hot-electron injection. We also discuss the main issues in PEC biosensors, including interference reduction, biocompatibility, material stability, and reproducibility. Lastly, we discuss future directions, emphasizing new materials, innovative device designs, and potential applications in food safety, environmental monitoring, and point-of-care diagnostics. The goal of this thorough overview is to assist researchers in choosing and creating cutting-edge photoactive materials for high-performance PEC biosensors of the future.

Keywords

photo electrochemistry / biosensors / immunodetection / sensor technology

Acknowledgement

This work was financially supported by National Natural Science Foundation of China (Nos. 61971301, 62031022, 51975400), in part by the Central Guidance on Local Science and Technology Development Fund of Shanxi Province(No. YDZJSX2021A01), and Fundamental Research Program of Shanxi Province (No. 202303021212082).

Declaration of conflicting interests

The authors have no conflict of interests related to this publication.

References

[1] CHAIN C Y,FRANCHIN L,CISNEROS J S,et al. Impedimetric screen-printed immunosensor for the rapid detection of chagas disease. IEEE Sensors Journal, 2024, 24(2): 1167-1174.

[2] CHEN Z P,XU C,MA C Q,et al. Lightweight and flexible graphene foam composites for high-performance electromagnetic interference shielding. Advanced Materials, 2013, 25(9): 1296-1300.

[3] GE J C,JIA Q Y,LIU W M,et al. Red-emissive carbon dots for fluorescent, photoacoustic, and thermal theranostics in living mice. Advanced Materials, 2015, 27(28): 4169-4177.

[4] LIU Z Y,ZHAO Y S,YIN Z G. Low-power soft transistors triggering revolutionary electronics. The Innovation, 2024, 5(3): 100616.

[5] NIEDERBERGER E,PARNHAM M J,MAAS J,et al. 4 Ds in health research-working together toward rapid precision medicine. EMBO Molecular Medicine, 2019, 11(11): e10917.

[6] ZENG Y,WANG Y H,ZHANG Y Q. Review on research progress of photoelectrochemical biosensors. Micromachines, 2025, 16(11): 1293.

[7] ZHANG X R,GUO Y S,LIU M S,et al. Photoelectrochemically active species and photoelectroch emical biosensors. RSC Advances, 2013, 3(9): 2846-2857.

[8] BRAMMER K S,FRANDSEN C J,JIN S. TiO₂ nanotubes for bone regeneration. Trends in Biotechnology, 2012, 30(6): 315-322.

[9] GUO W X,XU C,WANG X,et al. Rectangular bunched rutile TiO₂ nanorod arrays grown on carbon fiber for dye-sensitized solar cells. Journal of the American Chemical Society, 2012, 134(9): 4437-4441.

[10] REN Y,LIU Z,POURPOINT F,et al. Nanoparticulate TiO₂(B): an anode for lithium-ion batteries. Angewandte Chemie, 2012, 124(9): 2206-2209.

[11] WANG G L,XU J J,CHEN H Y. Dopamine sensitized nanoporous TiO₂ film on electrodes: Photoelectrochemical sensing of NADH under visible irradiation. Biosensors and Bioelectronics, 2009, 24(8): 2494-2498.

[12] TU W W,DONG Y T,LEI J P,et al. Low-potential photoelectrochemical biosensing using porphyrin-functionalized TiO₂ nanoparticles. Analytical Chemistry, 2010, 82(20): 8711-8716.

[13] SANAEIFAR N,RABIEE M,ABDOLRAHIM M,et al. A novel electrochemical biosensor based on Fe₃O₄ nanoparticles-polyvinyl alcohol composite for sensitive detection of glucose. Analytical Biochemistry, 2017, 519: 19-26.

[14] MOHAMAD NOR N,RIDHUAN N S,ABDUL RAZAK K. Progress of enzymatic and non-enzymatic electrochemical glucose biosensor based on nanomaterial-modified electrode. Biosensors, 2022, 12(12): 1136.

[15] LE P H,KIEN N T,VAN C N. Recent advances in BiVO4- and Bi₂Te₃-based materials for high efficiency-energy applications//Bismuth-Advanced Applications and Defects Characterization.2018.DOI:10.5772/INTECHOPEN.75613.

[16] COOPER J K,GUL S, TOMA F M,et al. Indirect bandgap and optical properties of monoclinic bismuth vanadate. The Journal of Physical Chemistry C, 2015, 119(6): 2969-2974.

[17] SUN B,ZHANG K,CHEN L J,et al. A novel photoelectrochemical sensor based on PPIX-functionalized WO₃⁃rGO nanohybrid-decorated ITO electrode for detecting cysteine. Biosensors and Bioelectronics, 2013, 44: 48-51.

[18] LI B C,CHEN Y F,PENG A H,et al. Improved photoelectrochemical properties of tungsten oxide by modification with plasmonic gold nanoparticles for the non-enzymatic sensing of ethanol. Journal of Colloid and Interface Science, 2019, 537: 528-535.

[19] ABDELFATAH M,DARWESH N,HABIB M A,et al. Enhancement of structural, optical and photoelectrochemical properties of n-Cu₂O thin films with K ions doping toward biosensor and solar cell applications. Nanomaterials, 2023, 13(7): 1272.

[20] KANG Z,YAN X Q,WANG Y F,et al. Electronic structure engineering of Cu₂O film/ZnO nanorods array all-oxide p-n heterostructure for enhanced photoelectrochemical property and self-powered biosensing application. Scientific Reports, 2015, 5: 7882.

[21] BARAN T,VISIBILE A,BUSCH M,et al. Copper oxide-based photocatalysts and photocathodes: fundamentals and recent advances. Molecules, 2021, 26(23): 7271.

[22] TANG J,KONG B,WANG Y C,et al. Photoelectrochemical detection of glutathione by IrO₂⁃hemin⁃TiO₂ nanowire arrays. Nano Letters, 2013, 13(11): 5350-5354.

[23] WANG W J,BAO L,LEI J P,et al. Visible light induced photoelectrochemical biosensing based on oxygen-sensitive quantum dots. Analytica Chimica Acta, 2012, 744: 33-38.

[24] TANNE J,SCHÄFER D,KHALID W,et al. Light-controlled bioelectrochemical sensor based on CdSe/ZnS quantum dots. Analytical Chemistry, 2011, 83(20): 7778-7785.

[25] ZHANG X Y,XU F,ZHAO B Q,et al. Synthesis of CdS quantum dots decorated graphene nanosheets and non-enzymatic photoelectrochemical detection of glucose. Electrochimica Acta, 2014, 133: 615-622.

[26] WANG Y F,HU A G. Carbon quantum dots: synthesis, properties and applications. Journal of Materials Chemistry C, 2014, 2(34): 6921-6939.

[27] WILBRINK J,HUANG C C,DOHNALOVA K,et al. Critical assessment of wet-chemical oxidation synthesis of silicon quantum dots. Faraday Discussions, 2020, 222: 149-165.

[28] ZHAO Z F,BUTBUREE T,PEERAKIATKHAJOHN P,et al. Carbon quantum dots sensitized vertical WO₃ nanoplates with enhanced photoelectrochemical properties. ChemistrySelect, 2016, 1(11): 2772-2777.

[29] XING Y C,CHEN X X,JIN B X,et al. Photoelectrochemical aptasensors constructed with photosensitive PbS quantum dots/TiO₂ nanoparticles for detection of kanamycin. Langmuir, 2021, 37(12): 3612-3619.

[30] DAI W X,ZHANG L,ZHAO W W,et al. Hybrid PbS quantum dot/nanoporous NiO film nanostructure: preparation, characterization, and application for a self-powered cathodic photoelectrochemical biosensor. Analytical Chemistry, 2017, 89(15): 8070-8078.

[31] DU J,DU Z L,HU J S,et al. Zn–Cu–In–Se quantum dot solar cells with a certified power conversion efficiency of 11.6%. Journal of the American Chemical Society, 2016, 138(12): 4201-4209.

[32] PREMKUMAR S,NATARAJ D,BHARATHI G,et al. Highly responsive ultraviolet sensor based on ZnS quantum dot solid with enhanced photocurrent. Scientific Reports, 2019, 9: 18704.

[33] YUAN R,YAN B D,LAI C Y,et al. Carbon dot-modified branched TiO₂ photoelectrochemical glucose sensors with visible light response. ACS Omega, 2023, 8(24): 22099-22107.

[34] DEL BARRIO M,LUNA-LÓPEZ G,PITA M. Enhancement of biosensors by implementing photoelectrochemical processes. Sensors, 2020, 20(11): 3281.

[35] YUAN J L,ZHENG L,HAO C J. Role of pyridine in photoelectrochemical reduction of CO₂ to methanol at a CuInS₂ thin film electrode. RSC Adv, 2014, 4(74): 39435-39438.

[36] SHANGGUAN L,YAN C Y,ZHANG H,et al. A visible light inducing photoelectrochemical biosensor with high-performance based on a porphyrin-sensitized carbon nitride composite. New Journal of Chemistry, 2022, 46(39): 18680-18687.

[37] ZHAO C R,ZHANG L Y,WANG Q,et al. Porphyrin-based covalent organic framework thin films as cathodic materials for “on-off-on” photoelectrochemical sensing of lead ions. ACS Applied Materials & Interfaces, 2021, 13(17): 20397-20404.

[38] YAN B D,ZHAO X R,CHEN D L,et al. Enhanced photoelectrochemical biosensing performance for Au nanoparticle–polyaniline–TiO₂ heterojunction composites. RSC Advances, 2020, 10(72): 43985-43993.

[39] ZHANG L,ZHU Y C,ZHAO W W. Recent advances of nanostructured materials for photoelectrochemical bioanalysis. Chemosensors, 2022, 10(1): 14.

[40] GUPTA R. Plasmonic nanomaterials-based point-of-care biosensors. St. Iouis: Washington University in St. Iouis, 2022.

[41] GÜELL F,GALDÁMEZ-MARTÍNEZ A,MARTÍNEZ-ALANIS P R,et al. ZnO-based nanomaterials approach for photocatalytic and sensing applications: recent progress and trends. Materials Advances, 2023, 4(17): 3685-3707.

[42] ZAYED M,NASSER N,SHABAN M,et al. Effect of morphology and plasmonic on Au/ZnO films for efficient photoelectrochemical water splitting. Nanomaterials, 2021, 11(9): 2338.

[43] XU L,HE X Y,DONG J T,et al. A photoelectrochemical aptasensor for sensitively monitoring chloramphenicol using plasmon-driven AgNP/BiOCl composites. Analyst, 2020, 145(23): 7695-7700.

[44] RATCHFORD D C,DUNKELBERGER A D,VURGAFTMAN I,et al. Quantification of efficient plasmonic hot-electron injection in gold nanoparticle-TiO₂ films. Nano Letters, 2017, 17(10): 6047-6055.

[45] KANG Z,YAN X Q,WANG Y F,et al. Self-powered photoelectrochemical biosensing platform based on Au NPs@ZnO nanorods array. Nano Research, 2016, 9(2): 344-352.

[46] LIU W Q,DUAN W,JIA L Q,et al. Surface plasmon-enhanced photoelectrochemical sensor based on Au modified TiO₂ nanotubes. Nanomaterials, 2022, 12(12): 2058.

[47] SHU J,QIU Z L,LV S Z,et al. Plasmonic enhancement coupling with defect-engineered TiO(2-x): a mode for sensitive photoelectrochemical biosensing. Analytical Chemistry, 2018, 90(4): 2425-2429.

[48] YANG T,LI D Z,JIAN A Q,et al. Enhanced photoelectrochemical detection of alpha-fetoprotein using dipole image effect and lock-In amplification. IEEE Sensors Journal, 2023, 23(5): 4685-4693.

[49] CAO L,WANG P P,CHEN L,et al. A photoelectrochemical glucose sensor based on gold nanoparticles as a mimic enzyme of glucose oxidase. RSC Advances, 2019, 9(27): 15307-15313.

[50] WANG D D,GE C W,WU G A,et al. A sensitive red light nano-photodetector propelled by plasmonic copper nanoparticles. Journal of Materials Chemistry C, 2017, 5(6): 1328-1335.

[51] PARVEEN F,SANNAKKI B,JAGTAP C V. Electrodeposited copper nanoparticles: plasmon enhanced performance of TiO₂ for dye sensitized solar cell. Journal of Materials Science: Materials in Electronics, 2017, 28(6): 5082-5087.

[52] SEO D B,TRUNG T N,BAE S S,et al. Improved photoelectrochemical performance of MoS₂ through morphology-controlled chemical vapor deposition growth on graphene. Nanomaterials, 2021, 11(6): 1585.

[53] XIAO J W,ZHANG Y,CHEN H J,et al. Enhanced performance of a monolayer MoS2/WSe2 heterojunction as a photoelectrochemical cathode. Nano-Micro Letters, 2018, 10(4): 60.

[54] NARESH V,LEE N. A review on biosensors and recent development of nanostructured materials-enabled biosensors. Sensors, 2021, 21(4): 1109.

[55] KLOS-WITKOWSKA A,MARTSENYUK V. Stability in biosensors derived from domain map analysis of bibliometric data. Acta Biochimica Polonica, 2024, 71: 12196.

[56] LIU Q,LIU D,ALPUCHE-AVILES M A,et al. Editorial: Nanotechnology facilitated photo (electrochemcial) biosensors. Frontiers in Bioengineering and Biotechnology, 2023, 11: 1136565.

[57] ISO. Biological evaluation of medical devices—Biological evaluation medical devices. ISO 10993-1: 2002. 2003: 1.

[58] JARCZEWSKA M,GÓRSKI Ł,MALINOWSKA E. Electrochemical aptamer-based biosensors as potential tools for clinical diagnostics. Analytical Methods, 2016, 8(19): 3861-3877.

[59] WHITE R J,PHARES N,LUBIN A A,et al. Optimization of electrochemical aptamer-based sensors via optimization of probe packing density and surface chemistry. Langmuir, 2008, 24(18): 10513-10518.

[60] HUANG J,PETRESCU F I T,LI B,et al. A novel ratiometric photoelectrochemical biosensor based on front and back illumination for sensitive and accurate glutathione sensing. Biosensors, 2024, 14(6): 285.

[61] SAHA S,CHAN Y T,SOLEYMANI L. Enhancing the photoelectrochemical response of DNA biosensors using wrinkled interfaces. ACS Applied Materials & Interfaces, 2018, 10(37): 31178-31185.

[62] BLASKIEVICZ S F,SALVATI B S,CORREA A A,et al. Photoelectrochemical biosensors//Handbook of II-VI Semiconductor-Based Sensors and Radiation Detectors. Cham: Springer International Publishing, 2023: 567-588.

[63] WU D,TANG D P. Recent advances on portable photoelectrochemical biosensors for diagnostics. Electroanalysis, 2023, 35(12): e202300265.

[64] PARDO-YISSAR V,KATZ E,WASSERMAN J,et al. Acetylcholine esterase-labeled CdS nanoparticles on electrodes: photoelectrochemical sensing of the enzyme inhibitors. Journal of the American Chemical Society, 2003, 125(3): 622-623.

[65] SUDHAGAR P,GONZÁLEZ-PEDRO V,MORA-SERÓ I,et al. Interfacial engineering of quantum dot-sensitized TiO₂ fibrous electrodes for futuristic photoanodes in photovoltaic applications. Journal of Materials Chemistry, 2012, 22(28): 14228.

[66] SHI X J,ZHANG K,SHIN K,et al. Constructing inverse opal structured hematite photoanodes via electrochemical process and their application to photoelectrochemical water splitting. Physical Chemistry Chemical Physics, 2013, 15(28): 11717-11722.