Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9FA

Chenglu Zhao , Huanhuan Lou , Qian Liu , Siqi Pei , Qiansheng Liao , Zhenghe Li

Journal of Integrative Plant Biology ›› 2024, Vol. 66 ›› Issue (10) : 2079 -2082.

PDF
Journal of Integrative Plant Biology ›› 2024, Vol. 66 ›› Issue (10) : 2079 -2082. DOI: 10.1111/jipb.13741
Brief Communications

Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9FA

Author information +
History +
PDF

Cite this article

Download citation ▾
Chenglu Zhao, Huanhuan Lou, Qian Liu, Siqi Pei, Qiansheng Liao, Zhenghe Li. Efficient and transformation-free genome editing in pepper enabled by RNA virus-mediated delivery of CRISPR/Cas9FA. Journal of Integrative Plant Biology, 2024, 66(10): 2079-2082 DOI:10.1111/jipb.13741

登录浏览全文

4963

注册一个新账户 忘记密码

References

[1]

Cao, Y.,Zhang, K.,Yu, H.,Chen, S.,Xu, D.,Zhao, H.,Zhang, Z.,Yang, Y.,Gu, X.,Liu, X., et al. (2022). Pepper variome reveals the history and key loci associated with fruit domestication and diversification. Mol. Plant 15: 1744-1758.

[2]

Choi, B.,Kwon, S.J.,Kim, M.H.,Choe, S.,Kwak, H.R.,Kim, M.K.,Jung, C., and Seo, J.K. (2019). A plant virus-based vector system for gene function studies in pepper. Plant Physiol. 181: 867-880.

[3]

Chunthawodtiporn, J.,Hill, T.,Stoffel, K., and Van Deynze, A. (2018). Quantitative trait loci controlling fruit size and other horticultural traits in bell pepper (Capsicum annuum). Plant Genome 11: 160125.

[4]

Kim, S.,Park, M.,Yeom, S.I.,Kim, Y.M.,Lee, J.M.,Lee, H.A.,Seo, E.,Choi, J.,Cheong, K.,Kim, K.T., et al. (2014). Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species. Nat. Genet. 46: 270-278.

[5]

Kothari, S.L.,Joshi, A.,Kachhwaha, S., and Ochoa-Alejo, N. (2010). Chilli peppers-a review on tissue culture and transgenesis. Biotechnol. Adv. 28: 35-48.

[6]

Liu, Q.,Zhao, C.,Sun, K.,Deng, Y., and Li, Z. (2023). Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes. Mol. Plant 16: 616-631.

[7]

Qin, C.,Yu, C.,Shen, Y.,Fang, X.,Chen, L.,Min, J.,Cheng, J.,Zhao, S.,Xu, M.,Luo, Y., et al. (2014). Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization. Proc. Natl. Acad. Sci. U.S.A. 111: 5135-5140.

[8]

Wang, H.,Yu, R.,Zhu, Q.,Tian, Z., and Li, F. (2024). A highly sensitive biotin-based probe for small RNA northern blot and its application in dissecting miRNA function in pepper. Plant J. 118: 263-276.

[9]

Wu, L.,Wang, P.,Wang, Y.,Cheng, Q.,Lu, Q.,Liu, J.,Li, T.,Ai, Y.,Yang, W.,Sun, L., et al. (2019). Genome-wide correlation of 36 agronomic traits in the 287 pepper (Capsicum) accessions obtained from the SLAF-seq-based GWAS. Int. J. Mol. Sci. 20: 5675.

[10]

Zhou, Y.,Deng, Y.,Liu, D.,Wang, H.,Zhang, X.,Liu, T.,Wang, J.,Li, Y.,Qu, L.,Liu, F., et al. (2021). Promoting virus-induced gene silencing of pepper genes by a heterologous viral silencing suppressor. Plant Biotechnol. J. 19: 2398-2400.

RIGHTS & PERMISSIONS

2024 Institute of Botany, Chinese Academy of Sciences.

AI Summary AI Mindmap
PDF

192

Accesses

0

Citation

Detail

Sections
Recommended

AI思维导图

/