Bacterial communities in litter are more sensitive to high nitrogen addition than fungal communities in a Korean pine (Pinus koraiensis) plantation

Abstract

The decomposition of litter by microbial communities is essential for ecosystem functioning. High nitrogen deposition, interacting with the flexible nutrient features of litter, can disrupt microbial succession. However, little is known about the specific links between microbial assembly and nitrogen addition, particularly in the in-situ litter layer. In a Korean pine (Pinus koraiensis) plantation, we investigated how eight years of nitrogen addition (0, 20, 40 and 80 kg ha⁻¹ a⁻¹) affect litter layer microbial community, assessing changes in abiotic properties and microbial community succession. The findings revealed complex influences of nitrogen addition on litter abiotic properties throughout the decomposition stage, such as contrasting influences on NH₄⁺ and NO₃⁻, where NH₄⁺ was elevated but NO₃⁻ was decreased. The effect on microbial community structure and assembly was highly stage-dependent. In the early stage, bacterial assembly was driven by stochastic processes (dispersal limitation). During the middle and late stages, high nitrogen addition shifted bacterial assembly from predominantly deterministic processes (heterogeneous selection) to stochastic processes (drift). However, it did not affect the predominance of stochastic processes during fungal assembly (dispersal limitation and drift). Thus, the influences of nitrogen addition on bacterial and fungal networks were inconsistent, with the stage-specific sensitivity differences between bacteria and fungi. Specifically, high nitrogen addition decreased bacterial stability and complexity, but promoted fungal stability over time. However, it did suppress fungal niche differentiation in the late stage. These results demonstrate that high nitrogen conditions influence litter abiotic properties and the associated microbial traits, such as community assembly, particularly during the late decomposition stage.

Graphical Abstract

Keywords

Litter layer / Microbial community / Nitrogen addition / Community assembly / Network stability

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