Hydrogen peroxide as a systemic messenger in the photosynthetic induction of mulberry leaves

Abstract

Mulberry (Morus alba L.) seedlings were used to test hydrogen peroxide (H₂O₂) as a potential systemic messenger in photosynthetic induction. The upper leaf of mulberry was dark-adapted for 45 min and then illuminated with photosynthetically active radiation (PAR) of 1000 μmol m⁻² s⁻¹. Photosynthetic induction and H₂O₂ content in the lower leaf was measured. The results show that pre-illumination of the upper leaf promoted photosynthetic induction and increased endogenous H₂O₂ in the lower leaf. Without pre-illuminating upper leaf, exogenous H₂O₂ treatment on the lower leaf promoted photosynthetic induction. The application of diphenyleneiodonium and trichloroacetic acid on petioles of the upper leaf inhibited H₂O₂ increase in the lower leaf, indicating that H₂O₂ transport was from upper leaves to lower leaves through the phloem. The results show that H₂O₂ might serve as a signal messenger to promote rapid induction of photosynthesis of leaves in lower parts of the canopy and enable plants to use light energy more efficiently.

Keywords

Hydrogen peroxide / Photosynthetic induction / Mulberry seedlings / Systematic signal / Passway

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