Hyperspectral imaging technology to detect the vigor of thermal-damaged Quercus variabilis seeds

Abstract

This study investigated the feasibility of hyperspectral imaging techniques to estimate the vigor of heat-damaged Quercus variabilis seeds. Four thermal damage grades were classified according to heat treatment duration (0, 2, 5, and 10 h). After obtaining hyperspectral images with a 370–1042 nm hyperspectral imager that included visible and near infrared light, germination was tested to confirm estimates. The Savitzky–Golay (SG) second derivative was used to preprocess the spectrum to reduce any noise impact. The successive projections algorithm (SPA), principal component analysis, and local linear embedding algorithm were used to extract the characteristic spectral bands related to seed vigor. Finally, a model for seed vigor classification of Q. variabilis based on partial least squares support vector machine (LS-SVM) with different spectral data sets was developed. The results show that the spectrum after SG second derivative preprocessing was better for developing the model, and SPA performed the best among the three feature band selection methods. The combination SG second derivative-LS-SVM provided the best classification model for Q. variabilis seed vigor, with the prediction set reaching 98.81%. This study provides an important basis for rapid and nondestructive assessment of the vigor of heat-damaged seeds using hyperspectral imaging techniques.

Keywords

Seed vigor level / Quercus variabilis / Heat damage / Hyperspectral / Least squares support vector machine

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