Effects of Serratia marcescens (SM1) and its interaction with common biocontrol agents on the termite, Odontotermes formosanus (Shiraki)

Abstract

Odontotermes formosanus (Shiraki), a black-winged subterranean termite, is a common forest pest. A red pigment-producing bacterial strain isolated from the termite was identified as Serratia marcescens and named SM1. A bioassay of SM1 on O. formosanus show that the LD₅₀ ranged from 1.77 × 10⁴ to 10.82 × 10⁴ cells/termite over 21–39 h. Three biological control agents, Beauveria bassiana (2 × 10¹⁰ cells/mL), Metarhizium anisopliae (1 × 10¹⁰ cells/mL) and Bacillus thuringiensis (1.6 × 10⁸ IU/mL), were used for an O. formosanus bioassay. The results show that the insecticidal effect of B. bassiana was stronger than that of M. anisopliae. In addition, two mixtures were obtained by combining B. bassiana (2 × 10¹⁰ cells/mL) with SM1 (1.5 × 10¹⁰ cells/mL), and M. anisopliae (1 × 10¹⁰ cells/mL) with S. marcescens (SM1) (1.5 × 10¹⁰ cells/mL) in equal volumes. The results show that B. bassiana and SM1 was less effective than SM1 alone. However, the insecticidal effect of M. anisopliae and SM1 was better than that of M. anisopliae or SM1 individually. These studies provide an important contribution for termite biocontrol.

Keywords

Odontotermes formosanus (Shiraki) / Serratia marcescens strain SM1 / LD₅₀ / Biological control agents

References

[1] Babashpour S, Aminzadeh S, Farrokhi N, Karkhane A, Haghbeen K. Characterization of a chitinase (Chit62) from Serratia marcescens B₄A and its efficacy as a bioshield against plant fungal pathogens. Biochem Genet, 2012, 50: 722-735.

[2] Chen XW, Fan H, Chen R, Yu XJ, Lan HX, Sun BZ. Study of the pathogenicity on pests and the utility in fields of Serratia marcescens. J Tianjin Agric Coll, 2001, 8: 28-30. (in Chinese)

[3] Chakraborty U, Chakraborty BN, Chakraborty AP. Influence of Serratia marcescens TRS-1 on growth promotion and induction of resistance in Camellia sinensis against Fomes lamaoensis. J Plant Interact, 2010, 5: 261-272.

[4] Fu RJ, Qi XR, Feng K, Xia XL, Tang F. Identification and characteristics of a strain of Serratia marcescens isolated from the termites, Odontotermes formosanus. J Nanjing For Univ Nat Sci Ed, 2019, 43(1): 76-82. (in Chinese)

[5] Jin H, Ge SR, Tao Y, Ran HY, Liu SG. Identification of a pathogenic strain of locusts and its toxicity and pathology. Acta Microbiol Sin, 2005, 45: 172-176. (in Chinese)

[6] Lavania M, Chauhan PS, Chauhan SV, Nautiyal CS. Induction of plant defense enzymes and phenolics by treatment with plant growth-promoting rhizobacteria Serratia marcescens NBRI₁₂₁₃. Curr Microbiol, 2006, 52: 363-368.

[7] Liang YY, Cheng SD, Cheng ZX, Qin HG, Hu SX, Li XM. Advances in research on botanical insecticides for termite control. Chin J Appl Entomol, 2012, 49: 778-784. (in Chinese)

[8] Liu ZC, Lu YX, Liu JH, Ren PL. Study on Serratia marcescens-pathogenic bacteria of rice planthopper. Nat Enem Insects, 1988, 10: 34-38. (in Chinese)

[9] Nobutaka S, Masami N, Ken W, Katsymi A. Synergistic antifungal activity of the culture filtrates of Serratia marcescens strain B2 and chemical fungicides against the sclerotia viability of the rice sheath blight pathogen, Rhizoctonia solani. Biocontrol Sci, 2005, 10: 97-100.

[10] Regev A, Keller M, Strizhov N, Sneh B, Prudovsky E, Chet I, Ginzberg I, Koncz-Kalman Z, Koncz C, Schell J, Zilberstein A. Synergistic activity of a Bacillus thuringiensis δ-endotoxin and a bacterial endochitinase against Spodoptera littoralis larvae. Appl Environ Microbiol, 1996, 62: 3581-3586.

[11] Sampson MN, Gooday GW. Involvement of chitinase of Bacillus thuringinesis during pathogenesis in insects. Microbiology, 1998, 144: 2189-2194.

[12] Tao K (2006) Studies on purification, gene cloning and expression of insecticidal protein. Doctorial dissertation, Sichuan University, Chengdu, China

[13] Ting ASY, Meon S, Kadir J, Radu S, Singh G. Induction of host defence enzymes by the endophytic bacterium Serratia marcescens, in banana plantlets. Int J Pest Manag, 2010, 56: 183-188.

[14] Wang K, Yan PS, Cao LX, Ding QL, Shao C, Zhao TF. Potential of chitinolytic Serratia marcescens strain JPP1 for biological control of Aspergillus parasiticus and Aflatoxin. Biomed Res Int, 2013

[15] Wang L, Zhang Z, Ou XK, Zhang JF, Jing YB, Zhang ZY, Cao JX, Li YP, Ma SY, Li RB. Preliminary researches on the aphides bio-control with bacteria. Guangxi Agric Sci, 2010, 41: 226-230. (in Chinese)

[16] Xu HG, Peng HY. Delayed effects of chitinase of Serratia marcesens S₃ on Helicoverpa armigera in different instars. Acta Microbiol Sin, 2004, 44: 88-92. (in Chinese)

[17] Yang JY, Cao X, Ji CY, Zhao XF, Zhang MX. Inhibitory effects of Serratia marcescens isolate PS-1 on the increase of experimental population of Spodoptera exigua. Chin J Biol Control, 2015, 31: 501-507. (in Chinese)

[18] Yang Q, Zhou Y, Han GJ, Fang JC. Isolation, identification and active ingredient analysis of two pathogenic bacterial strains isolated from Spodoptera exigua. Jiangsu J Agric Sci, 2012, 28: 1267-1271. (in Chinese)

[19] Yin HX, Zhang J, Hou RT, Wang JD, Yang ZR. Isolation and identification of a chitinase-producing bacterium and its synergistic effect on locust biocontrol. Plant Prot, 2004, 30: 37-41. (in Chinese)

[20] Zhang DF, Yang PZ, Cao LL, Jiang ST. Optimization of shake flask cultivation conditions for prodigiosin production by Serratia marcescen. Food Sci, 2015, 36: 119-124.

[21] Zhang YJ, Tan J, Lin YQ. The effects on peritrophic membrane of Helicoverpa armigera treated by low temperature and chitinase. Chin J Biol Control, 2000, 16: 152-155. (in Chinese)