Seasonal dynamics of soil microbial biomass C and N of Keteleeria fortunei var. cyclolepis forests with different ages

Abstract

Soil microbial biomass is an important indicator to measure the dynamic changes of soil carbon pool. It is of great significance to understand the dynamics of soil microbial biomass in plantation for rational management and cultivation of plantation. In order to explore the temporal dynamics and influencing factors of soil microbial biomass of Keteleeria fortunei var. cyclolepis at different stand ages, the plantation of different ages (young forest, 5 years; middle-aged forest, 22 years; mature forest, 40 years) at the Guangxi Daguishan forest station of China were studied to examine the seasonal variation of their microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) by chloroform fumigation extraction method. It was found that among the forests of different age, MBC and MBN differed significantly in the 0–10 cm soil layer, and MBN differed significantly in the 10–20 cm soil layer, but there was no significant difference in MBC for the 10–20 cm soil layer or in either MBC or MBN for the 20–40 cm soil layer. With increasing maturity of the forest, MBC gradually decreased in the 0–10 cm soil layer and increased firstly and then decreased in the 10–20 cm and 20–40 cm soil layers, and MBN increased firstly and then decreased in all three soil layers. As the soil depth increased, both MBC and MBN gradually decreased for all three forests. The MBC and MBN basically had the same seasonal variation in all three soil layers of all three forests, i.e., high in the summer and low in the winter. Correlation analysis showed that MBC was significantly positively correlated with soil organic matter, total nitrogen, and soil moisture, whereas MBN was significantly positively correlated with soil total nitrogen. It showed that soil moisture content was the main factor determining the variation of soil microbial biomass by Redundancy analysis. The results showed that the soil properties changed continuously as the young forest grew into the middle-aged forest, which increased soil microbial biomass and enriched the soil nutrients. However, the soil microbial biomass declined as the middle-age forest continued to grow, and the soil nutrients were reduced in the mature forest.

Keywords

Microbial biomass / Soil microbial nitrogen / Soil microbial carbon / Seasonal variation / Artificial forest / Keteleeria fortunei var. cyclolepis

References

[1] An R, Gong JR, You X, Ge ZW, Duan QW, Yan X. Seasonal dynamics of soil microorganisms and soil nutrients in fast-growing populus plantation forests of different ages 4in Yili, Xinjiang, China. Chin J Plant Ecol, 2011, 35(4): 389-401. (in Chinese)

[2] Berg G, Smalla K. Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiol Ecol, 2009, 68(1): 1-13.

[3] Binkley D, Fisher R. Ecology and management of forest soils, 2012, New York: Wiley 151 153

[4] Classen AT, Overby ST, Hart SC, Koch GW, Whitham TG. Season mediates herbivore effects on litter and soil microbial abundance and activity in a semi-arid woodland. Plant Soil, 2007, 295(2): 217-227.

[5] Devi NB, Yadava PS. Seasonal dynamics in soil microbial biomass C, N and P in a mixed-oak forest ecosystem of Manipur, North-east India. Appl Soil Ecol, 2006, 31(3): 220-227.

[6] Fierer N, Schimel JP, Holden PA. Influence of drying-rewetting frequency on soil bacterial community structure. Microb Ecol, 2003, 45(1): 63-71.

[7] Freppaz M, Said-Pullicino D, Filippa G, Curtaz F, Celi L, Zanini E. Winter-spring transition induces changes in nutrients and microbial biomass in mid-alpine forest soils. Soil Biol Biochem, 2014, 78: 54-57.

[8] Huang RL, He YH, Jiang Y, Zhang JC, Liu F, Jiang Y. Relationships between meteorological factors and artificial forest growth of Keteleeria cyclolepis Flous in Guangxi. Guangxi For Sci, 2016, 45(3): 328-333.

[9] Jefferies RL, Walker NA, Edwards KA, Dainty J. Is the decline of soil microbial biomass in late winter coupled to changes in the physical state of cold soils?. Soil Biol Biochem, 2010, 42(2): 129-135.

[10] Kaiser C, Fuchslueger L, Koranda M, Gorfer M, Stange CF, Kitzler B, Rasche F, Strauss J, Sessitsch A, Boltenstern SZ, Richter A. Plants control the seasonal dynamics of microbial N cycling in a beech forest soil by belowground allocation. Ecology, 2011, 92(5): 1036-1051.

[11] Kaiser C, Franklin O, Dieckmann U, Richter A. Microbial community dynamics alleviate stoichiometric constraints during litter decay. Ecol Lett, 2014, 17(6): 680-690.

[12] Li SL, Fang X, Xiang WH, Sun WJ, Zhang SJ. Soil microbial biomass carbon and nitrogen concentrations in four subtropical forests in hilly region of central Hunan province, China. Sci Silva Sin, 2014, 50(5): 8-16.

[13] Li Y, Zhang LP, Fang SZ, Tian Y, Guo J. Variation of soil enzyme activity and microbial biomass in poplar plantations of different genotypes and stem spacings. J For Res, 2018, 29(4): 963-972.

[14] Lipson DA, Schmidt SK, Monson ARK. Carbon availability and temperature control the post-snowmelt decline in alpine soil microbial biomass. Soil Biol Biochem, 2000, 32(4): 441-448.

[15] Liu D, Fang SZ, Tian Y, Dun XJ. Seasonal and clonal variations of microbial biomass and processes in the rhizosphere of poplar plantations. Appl Soil Ecol, 2014, 78: 65-72.

[16] Liu XS, Jiang Y, Huang RL, Liu F, Xiao YF, Jiang Y, Wei SX. Variation in traits of cone and seed of Keteleeria fortunei var. cyclolepis and its relationship with environmental factors. Guihaia, 2017, 37(1): 118-126. (in Chinese)

[17] Oksanen L. Logic of experiments in ecology: is pseudoreplication a pseudoissue?. Oikos, 2001, 94(1): 27-38.

[18] Pandey CB, Singh GB, Singh SK, Singh RK. Soil nitrogen and microbial biomass carbon dynamics in native forests and derived agricultural land uses in a humid tropical climate of India. Plant Soil, 2010, 333(1): 453-467.

[19] Ravindran A, Yang SS. Effects of vegetation type on microbial biomass carbon and nitrogen in subalpine mountain forest soils. J Microbiol Immunol, 2015, 48(4): 362-369.

[20] Ruan HH, Zou XM, Scatena FN, Zimmerman JK. Asynchronous fluctuation of soil microbial biomass and plant litterfall in a tropical wet forest. Plant Soil, 2004, 260(1): 147-154.

[21] Song P, Ren H, Jia Q, Guo J, Zhang N, Ma K. Effects of historical logging on soil microbial communities in a subtropical forest in southern China. Plant Soil, 2015, 397(2): 115-126.

[22] Stockdale EA, Brookes PC. Detection and quantification of the soil microbial biomass impacts on the management of agricultural soils. J Agric Sci, 2006, 144(4): 285-302.

[23] Trap J, Laval K, Akpa-Vinceslas M, Gangneux C, Bureau F, Decaëns T, Aubert M. Humus macro-morphology and soil microbial community changes along a 130-yr-old Fagus sylvatica, chronosequence. Soil Biol Biochem, 2011, 43(7): 1553-1562.

[24] Vance ED, Brookes PC, Jenkinson DS. An extraction method for measuring soil microbial biomass C. Soil Biol Biochem, 1987, 19(6): 703-707.

[25] Vidyanagar V. Seasonal and temporal variation in soil microbial biomass C, N and P in different types land uses of dry deciduous forest ecosystem of Udaipur, Rajasthan. Appl Ecol Environ Res, 2010, 8(4): 377-390.

[26] Wang X, Xu L, Wan R, Chen Y. Seasonal variations of soil microbial biomass within two typical wetland areas along the vegetation gradient of Poyang Lake, China. Catena, 2016, 137: 483-493.

[27] Wen L, Lei P, Xiang W, Yan W, Liu S. Soil microbial biomass carbon and nitrogen in pure and mixed stands of Pinus massoniana, and Cinnamomum camphora, differing in stand age. Forest Ecol Manag, 2014, 328: 150-158.

[28] Wu R, Kang FF, Han HR, Cheng XQ, Zhou WS, Wang LX, Chen J, Tian P. Soil microbial properties in Larix principis-rupprechtii plantations of different ages in Mt. Taiyue, Shanxi, China. Chin J Ecol, 2016, 35(12): 3183-3190.

[29] Xu X, Thornton PE, Post WM. A global analysis of soil microbial biomass carbon, nitrogen and phosphorus in terrestrial ecosystems. Glob Ecol Biogeogr, 2013, 22(6): 737-749.

[30] Yang K, Zhu JJ, Zhang JX, Yan QL. Seasonal dynamics of soil microbial biomass C and N in two larch plantation forests with different ages in Northeastern China. Acta Ecol Sin, 2009, 29(10): 5500-5507. (in Chinese)

[31] Yang K, Zhu J, Zhang M, Yan Q, Sun J. Soil microbial biomass carbon and nitrogen in forest ecosystems of Northeast China: a comparison between natural secondary forest and larch plantation. J Plant Ecol, 2010, 3(3): 175-182.

[32] Yao H, Bowman D, Shi W. Seasonal variations of soil microbial biomass and activity in warm- and cool-season turfgrass systems. Soil Biol Biochem, 2011, 43(7): 1536-1543.

[33] Zhou ZH, Wang CK. Soil resources and climate jointly drive variations in microbial biomass carbon and nitrogen in China’s forest ecosystems. Biogeosciences, 2015, 12(14): 11191-11216.