Molecular cloning, characterization, and antioxidant function of catalase in Lymantria dispar asiatic (Lepidoptera: Lymantriidae) under avermectin stress

Abstract

The critical antioxidant catalase (CAT) breaks down hydrogen peroxide induced by environmental stresses. Here we cloned full length catalase cDNA from Lymantria dispar asiatic (LdCAT). Bioinformatic analyses showed that open reading frames of LdCAT contains 1524 bp, encoding 507 amino acids with molecular weight of 126.99 kDa, theoretical pI of 5.00, aliphatic index of 29.92, grand average of hydropathicity of 0.764, and instability index (II) of 46.56. Protein BLAST and multiple sequence alignment indicated that LdCAT had high identity with CAT from other insects, especially lepidopterans. In a phylogenetic analysis, LdCAT was most similar to CAT from Spodoptera litura and S. exigua. Quantitative real-time polymerase chain reaction showed that LdCAT transcripts in all instar larvae and the five tissues tested, verifying the ubiquity of LdCAT in L. disapr. Moreover, LdCAT of third instar larvae was significantly upregulated after they fed on avermectin at sublethal and LC₁₀ doses. The highest relative transcript levels were found 2 h after an avermectin spray at LC₉₀, and in the cuticula, rather than heads, fat bodies, malpighian tubes, and midguts after a spray avermectin at a sublethal concentration. The expression level of LdCAT under pesticide stresses here suggested that CAT is an important antioxidant enzyme of L. disapr defensing against pesticide stress and may be a good target for controlling this pest.

Keywords

Lymantria dispar asiatic / Gypsy moth / Catalase / Molecular cloning / Relative expression level / Pesticide stress / Instar

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