Reproductive biology of Cornus capitata Wall. ex Roxb.: a native species in East Asia

Abstract

The reproductive biology of Cornus capitata was studied in detail from June 2014 to October 2015 in Tehri Garhwal, Uttarakhand, western Himalaya, India and elucidated floral morphology, confirming autogamy, geitonogamy, and xenogamy. A new inflorescence ball formed each year with developing fruits that were initiated the previous year; thus reproduction is a continuous cycle. The total pollen production within a tree ranged between 4.0 × 10⁷ and 9.3 × 10⁷. Anthesis peaked between 08:00 and 10:00. There was significant effect of micro-environmental condition on anthesis (F = 243.8, p = 3.01). A significant positive relationship between the number of lateral shoots and total inflorescence showed that the inflorescence production in C. capitate depends largely on the number of lateral shoots. Time and concentration of various solutions significantly affected pollen germination (time: F = 5.39, p = 0.002; concentration: F = 1.40, p = 0.234). Anthophora bees and thrips were observed as the main pollinators. There was a significant difference in seed set between natural and obligate self-pollination (t = 11.84, p = 1.99). Significant effect was also observed on fruit size (t = 8.88, p = 2.09) between open and shaded conditions. SEM micrographs of pollen grains showed that the shape of dehydrated pollen grains differed greatly due to variations in the rate of dehydration among pollen grains within the anther. Seed germination was observed only in quality seed derived from open pollination, supporting xenogamy in C. capitata. Findings of the present study will be of immense value in formulating effective conservation measures for the species in wild habitats.

Keywords

Floral development / Pollen production / Pollination / Pollinators / Reproductive success / SEM analysis

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