Drought stress tolerance analysis of Populus ussuriensis clones with different ploidies

Abstract

The selection of drought-tolerant plants is an important aspect of plant breeding. We studied physiological and biochemical mechanisms of different ploidies of Populus ussuriensis Kom. that relate to drought stress tolerance. We used a 5% (v/v) polyethylene glycol (PEG-6000) solution to simulate drought stress. We recorded leaf phenotypes including color, dry area and curl degree. We evaluated sequential variations in some drought stress tolerance-related physiological and biochemical indices and compared these among diploid clones (CK), triploid clones (T12) and tetraploid clones (F20). T12 leaves exhibited slightly more drought stress damage than CK and F20 leaves. CK leaves suffered the most severe drought stress damage. The physiological and biochemical indices of the different ploidies differed significantly 12 days after drought stress treatment. The activities of superoxide dismutase, peroxidase, catalase and proline in the triploid (T12) leaves were the highest. The relative electric conductivity and malondialdehyde content of T12 leaves were the lowest. The index values of F20 were between those of the diploid and triploid. In consideration of these results, the drought resistance of the three different ploidies of P. ussuriensis can be ranked as T12 > F20 > CK. We speculate that the gene expression patterns of polyploid clones of poplar will change after genome doubling and that some of the drought stress tolerance-related physiological and biochemical indices will be improved, resulting in greater drought tolerance of polyploid clones.

Keywords

Populus ussuriensis / Polyploidy / Drought stress / Tolerance

References

[1] Abuduwaili J, Zhang ZY, Jiang FQ, Liu DW (2015) The disastrous effects of salt dust deposition on cotton leaf photosynthesis and the cell physiological properties in the Ebinur Basin in Northwest China. PloS one 10:e0124546. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4430278/

[2] Alscher RG, Erturk N, Heath LS. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot, 2002, 53: 1331-1341.

[3] Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W. Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res, 2011, 6: 2026-2032.

[4] Bajji M, Kinet JM, Lutts S. The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul, 2002, 36: 61-70.

[5] Bréda N, Huc R, Granier A, Dreyer E. Temperate forest trees and stands under severe drought: a review of ecophysiological responses, adaptation processes and long-term consequences. Ann For Sci, 2006, 63: 625-644.

[6] DaMatta FM. Exploring drought tolerance in coffee: a physiological approach with some insights for plant breeding. Braz J Plant Physiol, 2004, 16: 1-6.

[7] Dhindsa RS, Plumb-Dhindsa P, Thorpe TA. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot, 1981, 32: 93-101.

[8] Eifler I. The individual results of crosses between B. verrucosa and B. pubescens. Silvae Genetica, 1960, 9: 159-165.

[9] Fan HF, Du CX, Guo SR. Effect of nitric oxide on proline metabolism in cucumber seedlings under salinity stress. J Am Soc Hortic Sci, 2012, 137: 127-133.

[10] Fang GG, Deng YJ, Li P (2001) Evaluation of pulping performance of triploid Populus tomentosa. For Sci Technol Manag (Suppl.):87–90

[11] Flowers TJ. Improving crop salt tolerance. J Exp Bot, 2004, 55: 307-319.

[12] Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem, 2010, 48: 909-930.

[13] Gong H, Zhu X, Chen K, Wang S, Zhang C. Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci, 2005, 169: 313-321.

[14] Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics. Article ID 701596. https://www.researchgate.net/publication/262114029_Mechanism_of_Salinity_Tolerance_in_Plants_Physiological_Biochemical_and_Molecular_Characterization. Accessed 3 Apr 2014

[15] Hasheminasab H, Assad MT, Aliakbari A, Sahhafi SR. Influence of drought stress on oxidative damage and antioxidant defense systems in tolerant and susceptible wheat genotypes. J Agric Sci, 2012, 4: 20.

[16] He C, Zhang W, Gao Q, Yang A, Hu X, Zhang J. Enhancement of drought resistance and biomass by increasing the amount of glycine betaine in wheat seedlings. Euphytica, 2011, 177: 151-167.

[17] He Q, Zhao S, Ma Q, Zhang Y, Huang L, Li G, Hao L. Endogenous salicylic acid levels and signaling positively regulate Arabidopsis response to polyethylene glycol-simulated drought stress. J Plant Growth Regul, 2014, 33: 871-880.

[18] Hsiao TC. Plant responses to water stress. Ann Rev Plant Physiol, 1973, 24: 519-570.

[19] Johnsson H. Triplody in Betula alba L. Bot Notiser Lund, 1944, 1: 85-96.

[20] Jung S. Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Sci, 2004, 166: 459-466.

[21] Li SW, Zhang ZY, Luo JM, He CZ, Pu YS, An XM. Progress and strategies in cross breeding of poplars in China. For Stud China, 2005, 7: 54-60.

[22] Li RH, Guo PG, Michael B, Stefania G, Salvatore C. Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agric Sci China, 2006, 5: 751-757.

[23] Lisar SYS, Motafakkerazad R, Hossain MM, Rahman IMM. Rahman IMM. Water stress in plants: causes, effects and responses. Water stress, 2012, London: InTech 635 642

[24] Liu W, Yu K, He T, Li F, Zhang D, Liu J (2013) The low temperature induced physiological responses of Avena nuda L., a cold-tolerant plant species. Sci World J. Article ID 658793. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693167/. Accessed 11 June 2013

[25] Martin MA, Ramos S, Mateos R, Granado Serrano AB, Izquierdo-Pulido M, Bravo L, Goya L. Protection of human HepG2 cells against oxidative stress by cocoa phenolic extract. J Agric Food Chem, 2008, 56: 7765-7772.

[26] Michalak A. Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Polish J Environ Stud, 2006, 15: 523-530.

[27] Morgan JM. Osmoregulation and water stress in higher plants. Ann Rev Plant Physiol, 1984, 35: 299-319.

[28] Niwa Y, Sasaki Y. Plant self-defense mechanisms against oxidative injury and protection of the forest by planting trees of triploids and tetraploids. Ecotoxicol Environ Saf, 2003, 55: 70-81.

[29] Osmond CB, Bjorkman O, Anderson DJ. Physiological processes in plant ecology. Toward a synthesis with atriplex. Agro-Ecosystems, 1980, 8(3): 268-271.

[30] Otto SP, Whitton J. Polyploid incidence and evolution. Ann Rev Genet, 2000, 34: 401-437.

[31] Pan Y, Wu LJ, Yu ZL. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regul, 2006, 49: 157-165.

[32] Panchuk II, Volkov RA, Schöffl F. Heat stress-and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiol, 2002, 129: 838-853.

[33] Pembrey RS, Marshall KC, Schneider RP. Cell surface analysis techniques: What do cell preparation protocols do to cell surface properties?. Appl Environ Microbiol, 1999, 65: 2877-2894.

[34] Pérez-Pérez JG, Syvertsen JP, Botia P, García-Sánchez F. Leaf water relations and net gas exchange responses of salinized Carrizo citrange seedlings during drought stress and recovery. Ann Bot, 2007, 100: 335-345.

[35] Ramsey J. Polyploidy and ecological adaptation in wild yarrow. Proc Natl Acad Sci U S A, 2011, 108: 7096-7101.

[36] Randolph LF. An evaluation of induced polyploidy as a method of breeding crop plants. Am Nat, 1941, 75: 347-363.

[37] Sairam RK, Srivastava GC. Water stress tolerance of wheat (Triticum aestivum L.): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. J AgronCrop Sci, 2001, 186: 63-70.

[38] Sita GL, Ram NVR, Vaidyanathan CS. Triploid plants from endosperm cultures of sandalwood by experimental embryogenesis. Plant Sc Lett, 1980, 20: 63-69.

[39] Sivakumaran S, Horgan R, Heald J, Hall MA. Effect of water stress on metabolism of abscisic acid in Populus robusta × schnied and Euphorbia lathyrus L. Plant, Cell Environ, 1980, 3: 163-173.

[40] Stebbins GL. Stebbins GL. Variation and evolution in plants: progress during the past twenty years. Essays in evolution and genetics in honor of Theodosius Dobzhansky: a supplement to evolutionary biology, 1970, New York: Appleton-Century-Crofts 173 208

[41] Storchova Z, Pellman D. From polyploidy to aneuploidy, genome instability and cancer. Nat Rev Mol Cell Biol, 2004, 5: 45-54.

[42] Sutka J, Farshadfar E, Kőszegi B, Friebe B, Gill BS. Drought tolerance of disomic chromosome additions of Agropyron elongatum to Triticum aestivum. Cereal Res Commun, 1995, 23: 351-357.

[43] Toivonen PMA, Sweeney M. Differences in chlorophyll loss at 13 C for two broccoli (Brassica oleracea L.) cultivars associated with antioxidant enzyme activities. J Agric Food Chem, 1998, 46: 20-24.

[44] Tsugane K, Kobayashi K, Niwa Y, Ohba Y, Wada K, Kobayashi H. A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. Plant Cell, 1999, 11: 1195-1206.

[45] Weimberg R, Lerner HR, Poljakoff-Mayber A. Changes in growth and water-soluble solute concentrations in Sorghum bicolor stressed with sodium and potassium salts. Physiol Plant, 1984, 62: 472-480.

[46] Xiong L, Zhu JK. Regulation of abscisic acid biosynthesis. Plant Physiol, 2003, 133: 29-36.

[47] Xu ZZ, Zhou GS. Effects of water stress and high nocturnal temperature on photosynthesis and nitrogen level of a perennial grass Leymus chinensis. Plant Soil, 2005, 269: 131-139.

[48] Yan B, Dai Q, Liu X, Huang S, Wang Z. Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant Soil, 1996, 179: 261-268.

[49] Zhang J, Kirkham MB. Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol, 1996, 132: 361-373.

[50] Zhu ZT, Kang XY. Studies on selection of natural triploids of Populus tomentosa. Scientia Silvae Sinicae, 1998, 34: 22-31.