Screening and verification of the factors influencing somatic embryo maturation of Larix olgensis

Abstract

With embryogenic callus of Larix olgensisis, we investigated the effects of inositol, glutamine, casein hydrolysate, carbohydrate, abscisic acid and silver nitrate concentration on the maturation of the somatic embryo. Three dominant factors emerged, and we developed a response surface model based on the Box–Behnken design. We defined the optimal conditions for the maturation of somatic embryos. The contents of abscisic acid, silver nitrate, sucrose and casein hydrolysis significantly affected the amount of maturing embryos, but inositol, maltose and glutamine had no effect. By establishing a response surface model with multiple factors, we predicted that the optimal number of L. olgensis somatic embryos was 204 ± 4 g⁻¹ on basal medium, containing 18.28 mg L⁻¹ abscisic acid, 5.46 mg L⁻¹ silver nitrate and 82.67 g L⁻¹ sucrose. In the verification experiments, the addition of 20 mg L⁻¹ abscisic acid, 5 mg L⁻¹ silver nitrate and 80 g L⁻¹ sucrose to BM yielded an average of 202.06 somatic embryos per gram. These results should guide large-scale breeding of L. olgensis.

Keywords

Larix olgensis / Embryogenic callus / Somatic embryo maturation / Box–Behnken design

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