Biochemical changes associated with flowering in Bambusa arundinacea and Bambusa nutans

Abstract

Biochemical changes associated with flowering in Bambusa arundinacea Linn and Bambusa nutans Wall. ex Munro were analyzed. Gregarious flowering was initiated in natural areas and plantations of B. arundinacea in late 2014 and reached full bloom in early 2015, whereas sporadic flowering recorded during 2014–2015 in vegetatively propagated plants of B. nutans. Leaf and nodal shoot samples from flowering culms/shoots and nonflowering culms/shoots were collected in February 2015 at the initiation of fruit filling, then analyzed and compared for soluble sugars, phenols and peroxidase activity. In both species sugars were higher in leaves of flowering culms/shoots and lower in nodal shoots of flowering culms/shoots compared to the nonflowering. Phenols were lower in leaves and nodal shoots of flowering B. arundinacea, but higher in leaves and decreases in nodal shoots of B. nutans. Peroxidase activity increases in leaves and nodal shoots of B. arundinacea after flowering but increases in nodal shoots and decreases in leaves of B. nutans.

Keywords

Sugars / Phenols / Peroxidase activity / Flowering / Bambusa arundinacea / Bambusa nutans

References

[1] Ansari SA, Kumar S, Palanisami K. Peroxidase activity in relation to in vitro rhizogenesis and precocious flowering in Bambusa arundinacea. Curr Sci, 1996, 71: 358-359.

[2] Berthon JY, Maldiney R, Sotta B, Gaspar T, Boyer N. Endogenous levels of plant hormones during the course of adventitious rootings in cuttings of Seqiroiadendroiz giganteirni (Lindl.) in vitro. Biochem Physiol der Pflanzen, 1989, 184: 405-412.

[3] Boontawee B (1988) Status of bamboo research and development in Thailand. In: Rao IVR, Gnanaharan R, Sastry CB (eds) Bamboos: Current research. Proceedings of the international bamboo workshop. Canada: Kerala Forest Research Institute, Peechi India and International Development Research Centre, Canada, pp 12–14

[4] Bouazza A, Rambour S, Gaspar T, Legrand B. Peroxidases during the course of callusing and organ differentiation from root explants of Cichorium intybus. Biologia Plantarum, 1993, 35: 48-489.

[5] Bray HG, Thorpe WV. Analysis of phenolic compound of interest in metabolism. Methods Biochem Anal, 1954, 1: 27-52.

[6] Bystriakova N, Kapos V, Lysenko I, Stapleton C. Distribution and conservation status of forest bamboo biodiversity in the Asia-Pacific region. Biodivers Conserv, 2003, 12(9): 1833-1841.

[7] Das S, Rizvan Md, Basu SP, Das S. Therapeutic potentials of Bambusa bambos Druce. Indo Global J Pharm Sci, 2012, 2: 85-87.

[8] Dubois MK, Gilles KA, Hamilton JK, Rebers PA, Smith F. A colorimetric method for the determination of sugar. Ann Chem, 1956, 28: 350-356.

[9] Ebrahimzadeh H, Abrishamchi P. Changes in IAA, phenolic compounds, peroxidase, IAA oxidase and polyphenol oxidase in relation to flower formation in Crocus sativus. Russ J Plant Physiol, 2001, 48(2): 190-195.

[10] Fouche JG, Coumans MP. Relationship between peroxidase activity and flower localization along Vanilla planifolia vines. Biologia Plantarum, 1995, 37(4): 515-521.

[11] Gamble JS. The Bambuseae of British India. Ann R Botanical Garden, Calcutta, 1896, 7: 1-133.

[12] Garg RK, Sharma RK, Kothari RM. Some insight on the death of bamboo after flowering. Indian Forester, 1998, 124(5): 342-346.

[13] Gasper T, Penel C, Castillo FJ, Greppin H. A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiologia Plantarum, 1985, 64: 418-423.

[14] Gomez KA, Gomez AA. Statistical procedures for agricultural research, 1984, Singapore: Wiley 680p

[15] Greppin H. Greppin H, Penel C, Gaspar Th. Peroxidase as a tool in the study of the flowering process. Molecular and physiological aspects of plant peroxidases, 1986, Geneva: University of Geneva 334 339

[16] Han SJ. Plant flowering mechanism and regulation research progress. J Pengbeng Vocat Univ, 2003, 18(2): 32-37.

[17] Hong WX. Physiological metabolic analysis and key factors during Phyllostachys heteroclada Olive and Neosinocalamusaffinis King flowering. Eurasian J Forest Sci, 2015, 3(1): 22-28.

[18] Janzen DH. Why bamboo wait so long for flower?. Annu Rev Ecol Syst, 1976, 7: 347-391.

[19] John CK, Nadgauda RS. Bamboo flowering and famine. Curr Sci, 2002, 82(3): 261-262.

[20] Li CB. Research on giant panda’s main diet bamboo species, 1997, Guiyang: Guizhou Science and Technology Press 2 180

[21] Lokhande SD, Ogawa K, Tanaka A, Hara T. Effect of temperature on ascorbate peroxidase activity and flowering of Arabidopsis thaliana ecotypes under different light conditions. J Plant Physiol, 2003, 110(1): 57-64.

[22] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem, 1951, 193: 265-275.

[23] Mahadevan A, Shridhar R. Methods in physiological plant pathology (3rd edition), 1986, Madras: Sivakami Publications 208 210

[24] Mohammad R, Bolouri M, Van den Ende Wim. Sugars, the clock and transition to flowering. Front Plant Sci, 2013, 4(22): 1-6.

[25] Mukherjee AK, Ratha S, Dhar S, Debata AK, Acharya PK, Mandal S, Panda PC, Mahapatra AK. Genetic relationships among 22 taxa of Bamboo revealed by ISSR and EST-based random primers. Biochem Genet, 2010, 48: 1015-1025.

[26] Rama Rao N, Naithani SC, Singh YD. Physiological and biochemical changes associated with cotton fiber development ii. Auxin oxidizing system. Physiologia Plantarum, 1982, 55: 204-208.

[27] Sachs RM. Nutrient diversion: an hypothesis to explain the chemical control of Flowering. Hortic Sci, 1977 12 3 220

[28] Sharma YML (1980) Bamboo in the Asia Pacific Region. In: Lessard G, Chouinard A (eds) Bamboo research in asia. Proceeding of Workshop 28–30 May 1980. Ottawa, Canada: International Development Research Centre, International Unit of Forestry Research Organization. pp 99–120

[29] Sharma ML (1994) The flowering of bamboo: fallacies and facts. Bamboo in Asia and the Pacific. FAO publication no. 6, Thailand. Chiang Mai, pp 68–70

[30] Tewari DN (1992) A monograph on bamboo. International Book Distributors, Dehradun, India. Dehradun India. Valley offset printers and publishers, 498p

[31] Varmah JC, Bahadur KN. 1980. India- a country report. In: Lessard G, Chouinard A. (eds) Bamboo research in Asia. Proceeding of workshop 28–30 May 1980. Ottawa, Canada: International Development Research Centre, International Unit of Forestry Research Organization, Ottawa, Canada, pp 19–46

[32] Zhang Y, Liu QY. Proceedings on molecular mechanism of plant flower development. Chin Bull Botany, 2003, 20(5): 589-601.

[33] Zhou SJ, Xu ZH. The research progress on the sucrose invertase structure and function of plant. Biotechnology, 2002, 12(5): 47-48.