The effects of phenolic acid on nitrogen metabolism in Populus × euramericana ‘Neva’

Abstract

The declines in soil fertility and productivity in continuously cropped poplar plantations are related to phenolic acid accumulation in the soil. Nitrogen is a vital life element for poplar and whether the accumulation of phenolic acid could influence nitrogen metabolism in poplar and thereby hinder continuous cropping is not clear. In this study, poplar cuttings of Populus × euramericana ‘Neva’ were potted in vermiculite, and phenolic acids at three concentrations (0X, 0.5X and 1.0X) were added according to the actual content (1.0X) in the soil of a second-generation poplar plantation. Each treatment had eight replicates. We measured gas exchange parameters and the activities of key enzymes related to nitrogen metabolism in the leaves. Leaf photosynthetic parameters varied with the concentration of phenolic acids. The net photosynthetic rate (P _N) significantly decreased with increasing phenolic acid concentration, and non-stomatal factors might have been the primary limitation for P _N. The activities of nitrate reductase (NR), glutamine synthetase (GS) and glutamate synthase (GOGAT), as well as the contents of nitrate nitrogen, ammonium nitrogen, and total nitrogen in the leaves decreased with increasing phenolic acid concentration. This was significantly and positively related to P _N (P < 0.05). The low concentration of phenolic acids mainly affected the transformation process of NO₃ ⁻ to NO₂ ⁻, while the high concentration of phenolic acids affected both processes, where NO₃ ⁻ was transferred to NO₂ ⁻ and NH₄ ⁺ was transferred to glutamine (Gln). Overall, phenolic acid had significant inhibitory effects on the photosynthetic productivity of Populus × euramericana ‘Neva’. This was probably due to its influence on the activities of nitrogen assimilation enzymes, which reduced the amount of amino acids that were translated into protein and enzymes. Improving the absorption and utilization of nitrogen by plants could help to overcome the problems caused by continuous cropping.

Keywords

Continuous cropping management / Poplar / Phenolic acids / Photosynthesis / Nitrogen metabolism / Enzyme activity

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