Vegetation cover density and disturbance affected arbuscular mycorrhiza fungi spore density and root colonization in a dry Afromontane forest, northern Ethiopia

Abstract

Arbuscular mycorrhiza fungi (AMF) are vital in the regeneration of vegetation in disturbed ecosystems due to their numerous ecological advantages and therefore are good indicators of soil and ecosystem health at large. This study was aimed at determining how the seasonal, vegetation cover density, edaphic and anthropogenic factors affect AMF root colonization (RC) and spore density (SD) in Desa’a dry Afromontane forest. AMF RC and SD in the rhizosphere of five dominant woody species, Juniperus procera, Olea europaea, Maytenus arbutifolia, Carissa spinarum and Dodonaea angustifolia growing in Desa’a forest were studied during the rainy and the dry seasons in three permanent study vegetation cover density plots (dense, medium, and poor). Each plot (160 × 40 m²) has two management practices (fenced and unfenced plots) of area. A 100 g sample of rhizosphere soil from moisture-free composite soil was used to determine spore density. Spore density ranged from 50 to 4467 spores/100 g soil, and all species were colonized by AMF within a range of 4–95%. Glomus was the dominant genus in the rhizosphere of all species. Vegetation cover density strongly affected SD and RC. The SD was significantly higher (p < 0.05) in the poor vegetation cover density than in the other two and lowest in the dense cover; root colonization showed the reverse trend. Management practices significantly (p < 0.05) influenced AMF SD and RC, with the fenced plots being more favoured. Seasons significantly (p < 0.05) affected RC and SD. More RC and SD were observed in the wet period than the dry period. Correlating AMF SD and RC with soil physical and chemical properties showed no significant difference (p > 0.05) except for total nitrogen. Disturbance, vegetation cover density, season and total nitrogen are significant factors that control the dynamics and management interventions to maintain the forest health of dry Afromontane forests.

Keywords

AM fungi / Disturbance / Dry Afromontane forest / Season / Vegetation cover density

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