Assaying the allelopathic effects of Eucalyptus camaldulensis in a nursery bed incorporated with leaf litter

Abstract

Allelopathic effects of Eucalyptus camaldulensis Dehnh. were confirmed in Petri dish and pot experiments in our previous studies. However, the degree to which such effects under controlled experiments exist in more complex ecological settings remains to be tested. Thus, the present study was carried out by incorporating different proportions of ground litter of E. camaldulensis in soil. The growth of three agricultural crops: falen (Vigna unguiculata (L.) Walp.), chickpea (Cicer arietinum L.), and arhor (Cajanus cajan (L.) Millsp.), and two tree species, kala koroi (Albizia procera (Roxb.) Benth.) and ipil ipil (Leucaena leucocephala (Lam.) de Wit) were tested. There were inhibitory effects of leaf litter on germination, shoot and root growth, leaf number, and collar diameter as well as a reduction of nodulation by legume crops (25–80% reduction). The extent of the effects was dependent on the proportion of leaf litter, the species and the type of traits. In contrast to shoot growth, the effect on root growth was more severe. No effect on germination was found with the agriculture crops while the two tree species showed reduced germination. The effect was greater in the presence of higher proportions of leaf litter mixed in soil while in some cases lower proportions stimulated growth. Not all species were suppressed; A. procera, C. cajan, V. unguiculata showed compatible growth while C. arietinum and L. leucocephala were found incompatible. This study provides evidence that E. camaldulensis has allelopathic potential under field conditions and a careful selection of associated crops in agroforestry systems is highly recommended.

Keywords

Allelopathy / Eucalyptus camaldulensis / Inhibitory effects / Germination / Growth / Nodulation

References

[1] Abhilasha D, Quintana N, Vivanco J, Joshi J. Do allelopathic compounds in invasive Solidago canadensis s.l. restrain the native European flora?. J Ecol, 2008, 96: 993-1001.

[2] Adams MA, Attiwill PM. Nutrient cycling and nitrogen mineralization in eucalypt forests of south-eastern Australia. I. Nutrient cycling and nitrogen turnover. Pl Soil, 1986, 92: 319-339.

[3] Ahmed FU, Akhter S (1995) Problems and prospects of Eucalyptus. In: Amin SMR, Ali MO, Fattah MIM (eds.), Eucalyptus in Bangladesh, Proceedings of the National Seminar held at Bangladesh Agricultural Research Council, Dhaka on April 16.1994, pp 42–53

[4] Ahmed R, Uddin MB, Hossain MK. Allelopathic effects of leaf extracts of Eucalyptus camaldulensis Dehn. on agricultural crops. Bangladesh. J Bot, 2004, 33(2): 79-84.

[5] Ahmed R, Redowan M, Uddin MS, Hossain MK. A pot experiment to evaluate the allelopathic potential of leaf extracts of Eucalyptus camaldulensis on five common agricultural crops. Int J Sustain Agric Technol, 2007, 5(3): 280-285.

[6] Ahmed R, Redowan M, Uddin MS, Hossain MK. Eucalyptus as a agroforestry component in the homestead and agricultural field of Sitakunda, Bangladesh. Int J Sustain Agric Technol, 2007, 3(3): 46-51.

[7] Ahmed R, Uddin MB, Khan MS, Mukul SA, Hossain MK. Allelopathic effects of Lantana camara L. on germination and growth behavior of some agricultural crops. J For Res, 2007, 18(4): 301-304.

[8] Ahmed R, Hoque ATMR, Hossain MK. Allelopathic effects of leaf litters of Eucalyptus camaldulensis on some forest and agricultural crops. J For Res, 2008, 19(1): 19-24.

[9] Ameen M (1999) Development of guiding principles for the prevention of impacts of alien species. Paper presented at a consultative workshop in advance of the 4th Meeting of SBSTTA to the CBD, organized by IUCN Bangladesh at Dhaka on 25 May 1999

[10] Amin SMR, Ali MO, Fattah MIM. (eds.) (1995) Eucalypts in Bangladesh. Proceedings of a seminar held at the Bangladesh Agricultural Research Council, Dhaka on April 6, 1994. pp 73

[11] Ashrafi Z, Mashhadi HR, Sadeghi S, Alizade HM. Effects allelopathical of sunflower (Helianthus annuus) on germination and growth of wild varley (Hordeum spontaneum). J Agric Technol, 2008, 4(1): 219-229.

[12] Attiwill PM. Nutrient cycling in a Eucalyptus obliqua (L’Herit.) forest. III. Growth, biomass, and net primary production. Aust J Bot, 1979, 27(4): 439-458.

[13] Ballester A, Vieitez AM, Vieitez E. Allelopathic potential of Erica vegans, Calluna vulgaris and Daboecia cantabrica. J Chem Ecol, 1982, 8: 851-857.

[14] Bargali K, Bargali SS. Nutrient utilisation efficiencies of two central Himalayan tree species. J Trop For Sci, 2000, 12(3): 450-458.

[15] Bargali K, Bargali SS. Diversity and biomass of the understory vegetation in an age series of Eucalyptus tereticornis plantation. Int J Ecol Environ Sci, 2000, 26: 173-181.

[16] Bargali SS, Singh RP, Singh SP. Structure and function of an age series of eucalypt plantations in Central Himalaya, II. Nutrient dynamics. Ann Bot, 1992, 69(5): 413-421.

[17] Bargali SS, Singh SP, Singh RP. Structure and function of an age series of eucalypt plantations in Central Himalaya. I. Dry matter dynamics. Ann Bot, 1992, 69(5): 405-411.

[18] Bargali SS, Singh SP, Singh RP. Patterns of weight loss and nutrient release from decomposing leaf litter in an age series of eucalypt plantations. Soil Biol Biochem, 1993, 25: 1731-1738.

[19] Bradstock R. Biomass in an age series of Eucalyptus grandis plantations. Aust J For Res, 1981, II: 111-127.

[20] Catherine F, Benjamin L, Bruno V, Jean-Philippe M, Christine R, Stéphane G, Sylvie D, Anne BM. Potential allelopathic effect of Pinus halepensis in the secondary succession: an experimental approach. Chemoecology, 2006, 16: 97-105.

[21] Catherine F, Sébastien V, Jean-Philippe M, Bruno V, Elena O, Sylvie D, Anne BM. Regeneration failure of Pinus halepensis Mill: the role of autotoxicity and some abiotic environmental parameters. For Ecol Manag, 2008, 255: 2928-2936.

[22] Courtney KJ, Ward BB, Langenheim JH. The effect of coastal redwood monoterpenes on Nitrosomonas europaea. Am J Bot, 1991, 78: 144-145.

[23] Davidson J (1985) Ecological and social aspects of eucalypts when used as exotics. In: J. Davidson and S. Das (eds.), Eucalypts in Bangladesh—a Review. Silviculture Bulletin No. 6. Chittagong. Bangladesh Forest Research Institute, pp 13–49

[24] Davidson J, Das S (1985) Eucalypts in Bangladesh—A review. Bull. 6, Silv. Res. Div. Bangladesh Forest Research Institute, Chittagong, Bangladesh. Pp. 246

[25] Del Fabbro C, Gusewell S, Prati D. Allelopathic effects of three plant invaders on germination of native species: a field study. Biol Invasions, 2014, 16: 1035-1042.

[26] Djurdjević L, Popović Z, Mitrović M, Pavlović P, Jarić S, Oberan L, Gajić G. Dynamics of bioavailable rhizosphere soil phenolics and photosynthesis of Arum maculatum L. in a lime—beech forest. Flora, 2008, 203: 590-601.

[27] Einhelling FA. Interactions involving allelopathy in cropping systems. Agron J, 1996, 88: 886-893.

[28] Ens EJ, French K, Bremner JB. Evidence for allelopathy as a mechanism of community composition change by an invasive exotic shrub, Chrysanthemoides monilifera spp. Rotundata. Pl Soil, 2009, 316: 125-137.

[29] Haque SMS (2013) Geology, soil science and forest soils. Degradation of upland watershed in Bangladesh project funded by USDA, Institute of Forestry and Environmental Science, p 332

[30] Hasanuzzaman M, Hossain M, Saroar M. Diversity and Preference of agricultural crops in the cropland agroforests of southwestern Bangladesh. Int J Agric Crop Sci, 2004, 7(7): 364-372.

[31] Hassan MM. Prospects of Eucalypts in Bangladesh. Bangladesh J For Sci, 1994, 23(1): 12-19.

[32] Hong NH, Xuan TD, Eiji T, Khanh TD. Paddy weed control by higher plants from Southeast Asia. Crop Prot, 2004, 23: 255-261.

[33] Hoque ATM, Ahmed R, Uddin MB, Hossain MK. Allelopathic effect of different concentration of water extracts of Acacia auriculiformis on some initial growth parameters of five common agricultural crops. Pak J Agron, 2003, 2(22): 92-100.

[34] Hoque ATMR, Uddin MB, Ahmed R, Hossain MK. Suppressive effects of aqueous extracts of Azadirachta indica leaf on some initial growth parameters of six common agricultural crops. Asian J Pl Sci, 2003, 2(10): 738-742.

[35] Hossain MK, Hoque ATMR. Eucalyptus Dilemma in Bangladesh, 2013, Chittagong: Institute of Forestry and Environmental Sciences, University of Chittagong 148

[36] Inderjit. Soil microorganisms: an important determinant of allelopathic activity. Pl Soil, 2005, 274: 227-236.

[37] Jandová K, Dostá P, Cajtham T. Searching for Heracleum mantegazzianum allelopathy in vitro and in a garden experiment. Biol Invasions, 2015, 17: 987-1003.

[38] Jones JM, Richards BN. Effect of reforestation on turnover of ¹⁵N labelled nitrate and ammonium in relation to changes in soil micro-flora. Soil Biol Biochem, 1977, 9: 383-392.

[39] Kaur H, Kaur R, Kaur S, Baldwin IT Inderjit. Taking ecological function seriously: soil microbial communities can obviate allelopathic effects of released metabolites. PLoS ONE, 2009, 4: e4700.

[40] Keeley JE. Allelopathy. Ecology, 1988, 69: 292-293.

[41] Khan MA, Khitran TA, Baloch MS, Suiemani MZ. Allelo- pathic effect of eucalyptus on soil characteristics and growth of maize. Pak J Biol Sci, 1999, 2(1): 390-393.

[42] Lankau R. Soil microbial communities alter allelopathic competition between Alliaria petiolata and a native species. Biol Invasions, 2010, 12: 2059-2068.

[43] May FE, Ash JE. An assessment of the allelopathic potential of eucalyptus. Aust J Bot, 1990, 38: 245-254.

[44] Nektarios PA, Economou G, Avgoulas C. Allelopathic effects of Pinus halepensis needles on turfgrasses and biosensor plants. HortScience, 2005, 40(1): 246-250.

[45] Nishimura H, Kaku K, Nakamura T, Fukazawa Y, Mizutani J. Allelopathic Substances, (±)-p-Menthane-3,8-diols Iso- lated from Eucalyptus citriodora Hook. Agric Biol Chem, 1982, 46: 319-320.

[46] Powell KI, Chase JM, Knight TM. Invasive plants have scale-dependent effects on diversity by altering species area relationships. Science, 2013, 339: 316-318.

[47] Reigosa MJ, Gonzalez L, Souto XC, Pastoriza JE. Tauro P. Allelopathy in forest ecosystems. Allelopathy in ecological agriculture and forestry, 2000, Dordrecht: Kluwer 183 193

[48] Rice EL. Allelopathy, 1984 2 Orlando: Academic Press 422

[49] Shiva V, Bandyopadhyay J. Eucalyptus—a disastrous tree in India. The Ecologist, 1983 13 5 184187

[50] Si CC, Liu XY, Wang CY, Wang L, Dai ZC, Qi SS, Du DL. Different degrees of plant invasion significantly affect the richness of the soil fungal community. PLoS ONE, 2013, 8: e85490.

[51] Stowe LG. Allelopathy and its influence on the distribution of plants in an Illinois old-field. J Ecol, 1979, 67: 1065-1085.

[52] Sun ZK, He WM. Evidence for enhanced mutualism hypothesis: Solidago canadensis plants from regular soils perform better. PLoS ONE, 2010, 5: e15418.

[53] Surendra MP, Pota KB. On the allelopathic potentials of root exudates from different ages of Celosia argenta Linn. Natl Acad Sci Lett, 1978, 1(2): 56-58.

[54] Uddin MB, Ahmed R, Mukul SA, Hossain MK. Inhibitory effects of Albizia lebbeck (L.) Benth. leaf extracts on germination and growth behavior of some popular agricultural crops. J For Res, 2007, 18(2): 128-132.

[55] Ward BB, Courtney KJ, Langenheim JH. Inhibition of Nitrosomonas europaea by monoterpenes from coastal redwood (Sequoia sempervirens) in whole cell studies. J Chem Ecol, 1997, 23(11): 2583-2598.

[56] White CS. Volatile and water—soluable inhibitors of nitrogen mineralization and nitrification in a ponderosa pine ecosystem. Biol Fertil Soils, 1986, 2: 97-104.

[57] White CS. The role of monoterpenes in soil nitrogen cycling processes in ponderosa pine. Biogeochemistry, 1991, 12: 43-68.

[58] Wood SE (1996) Loss of foliar monoterpenes from Umbellularia californica leaf litter and their influence on nitrification potential in soil beneath the trees. PhD dissertation. University of California, Santa Cruz, p 151

[59] Yang RY, Mei LX, Tang JJ, Chen X. Allelopathic effects of invasive Solidago canadensis L. on germination and root growth of native Chinese plants. Allelopath J, 2007, 19: 241-248.

[60] Yuan YG, Wang B, Zhang SS, Tang JJ, Tu C, Hu SJ, Yong JWH, Chen X. Enhanced allelopathy and competitive ability of invasive plant Solidago canadensis in its introduced range. J Pl Ecol, 2013, 6: 253-263.

[61] Zhang JH, Mao ZQ, Wang LQ, Shu HR. Bioassay and identification of root exudates of three fruit tree species. J Integr Pl Biol, 2007, 49: 257-261.

[62] Zhang D, Zhang J, Yang W, Wu F. Potential allelopathic effect of Eucalyptus grandis across a range of plantation ages. Ecol Res, 2010, 25: 13-23.