Characterization of a collection of Chinese fir elite genotypes using sequence-related amplified polymorphism markers

Abstract

Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a major timber tree species in China. In the present study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese fir elite genotypes (n = 103) from the Lechang provenance, an endangered provenance. Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes; 577 (93.1 %) of these were polymorphic, yielding an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations (n = 35), 14 ones identified ≥70.0 % of the 120 genotypes. The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes (87.4 %) grouped together (Cluster A). The Lechang genotypes had a considerable amount of genetic variation with genetic similarity from 0.40 to 0.81. The current work may facilitate the management of the endangered Lechang Chinese fir provenance.

Keywords

Chinese fir / SRAP / Germplasm / Genetic variation / Dendrogram

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