Micropropagation of yellow mangosteen: a valuable endemic tree of India

Abstract

We developed a method for in vitro regeneration of Garcinia xanthochymus (yellow mangosteen) from matured seed segments. Multiple shoots were induced on woody plant (WP) medium supplemented with cytokinins. An average of 11 shoots per explant were regenerated from mature seed segments on WP medium containing 20 µM 6-benzylaminopurine. Histological analysis revealed that hypodermal cells of seed segments were initially involved in active division, which later developed into meristemoids, subsequently leading to the formation of shoot buds. Shoot elongation was achieved by repeated subculturing of seed explants in shoot regeneration medium. Rooting of shoots was achieved on WP medium supplemented with indole-3-butyric acid or α-naphthalene acetic acid. Plantlets were transplanted to pots containing soil: compost (1:1) and survival rate was 90 %.

Keywords

Adventitious shoots / Garcinia xanthochymus / In vitro propagation / Tissue culture / WP medium

References

[1] Baggett S, Protiva P, Mazzola EP, Yang H, Ressler ET, Basile MJ, Weinstein IB, Kennelly EJ. Bioactive benzophenones from Garcinia xanthochymus fruits. J Nat Prod, 2005, 68(3): 354-360.

[2] Baskaran M, Krishnan S. High frequency plant regeneration from the mature seeds of Garcinia indica. Biol Plant, 2011, 55(3): 554-558.

[3] Deodhar SR, Thengane RJ, Thengane SR. De nova shoot regeneration from root culture of Garcinia indica Choiss. Indian J Exp Biol, 2008, 46(6): 482-486.

[4] Facciola S. Cornucopia II: a source book of edible plants. 1998, California: Kampong Publications, 79.

[5] Goh HKL, Rao AN, Loh CS. Direct shoot bud formation from leaf explant of seedlings and mature mangosteen (Garcinia mangostana L.) trees. Plant Sci, 1990, 68(1): 113-121.

[6] Hiregoudar LV, Murthy HN, Hema BP, Hahn EJ, Paek KY. Multiple shoot induction and plant regeneration of Feronia limonia (L.) Swingle. Sci Hortic, 2003, 98(4): 357-364.

[7] Hiregoudar LV, Murthy HN, Bhat JG, Nayeem A, Hema BP, Hahn EJ, Paek KY. Rapid clonal propagation of Vitex trifolia. Biol Plant, 2006, 50(2): 291-294.

[8] Huang LC, Huang BL, Wang CH, Kuo CI, Murashige T. Developing improved in vitro propagation system for slow-growing species using Garcinia mangostana L. (mangosteen). In Vitro Cell Dev Biol Plant, 2000, 36: 501-504.

[9] Lloyd GB, McCrown BH. Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Comb Proc Int Plant Propag Soc, 1980, 30: 412-427.

[10] Mabberly DJ. The plant book: a portable dictionary of the higher plants. 1993, New York: Cambridge University Press, 236.

[11] Malik SK, Chaudhury R, Kalia RK. Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hortic, 2005, 106(4): 519-523.

[12] Park SY, Kim YW, Moon HK, Murthy HN, Choi YH, Cho HM. Micropropagation of Salix pseudolasiogyne from nodal explants. Plant Cell Tissue Organ Cult, 2008, 93(3): 341-346.

[13] Thengane SR, Deodhar SR, Bhosle SV, Rawal SK. Direct somatic embryogenesis and plant regeneration in Garcinia indica Choisy. Curr Sci, 2006, 91(8): 1074-1078.