Variations in electrical impedance and phase angle among seedlings of Pinus densata and parental species in Pinus tabuliformis habitat environment

Abstract

Electrical impedance (EI) and phase angle (PHI) parameters in AC impedance spectroscopy are important electrical parameters in the study of medical pathology. However, little is known about their application in variation and genetic relationship studies of forest trees. In order to test whether impedance parameters could be used in genetic relationship analysis among conifer species, EI and PHI were measured in a seedling experiment test composed of Pinus tabuliformis, Pinus yunnanensis, and Pinus densata in a habitat of Pinus tabuliformis. The results showed that variations in both EI and PHI among species were significant in different electric frequencies, and the EI and PHI values measured in the two populations of P. densata were between the two parental species, P. yunnanensis and P. tabuliformis. These results show that these two impedance parameters could reflect the genetic relationship among pine species. This was the first time using the two AC impedance spectroscopy parameters to test the genetic relationship analysis between tree species, and would be a hopeful novel reference methodology for future studies in evolution and genetic variation of tree species.

Keywords

Electrical impedance / Phase angle / Pinus tabuliformis / Pinus yunnanensis / Pinus densata / Seedling needle / Genetic variation

References

[1] Cai Q, Zhang D, Liu Z-L, Wang X-R. Chromosomal localization of 5S and 18S rDNA in five species of subgenus Strobus and their implications for genome evolution of Pinus. Ann Bot, 2006, 97(5): 715-722.

[2] Chen ZY, Zhang JP, Liu YL, Wang RQ, Wang GD. Relationships between Tomato maturity and dielectric parameters. Acta Bot Boreal Occident Sin, 2008, 28(4): 826-830.

[3] Cox MA, Zhang M, Willison J. Apple bruise assessment through electrical impedance measurements. J Hortic Sci (United Kingdom), 1993, 68(3): 393-398.

[4] Cseresnyés I, Rajkai K, Vozáry E. Role of phase angle measurement in electrical impedance spectroscopy. Int. Agrophys, 2013, 27: 377-383.

[5] Dai JF, Meng JX, Mao JF, Zhao W, Liu H, Xing Z, Zhang H, Wang XR, Li Y. Comparison analysis of seedling traits for hybrids between Pinus tabuliformis × P.yunnanensis and its parental species in P.densata habitat site. J Beijing Forest Univ, 2014, 36(1): 8-14.

[6] Du GY, Tang Y, Guo AG, Zhang JP. Influence of frequency signal on electrical property of peach fruits. J Food Sci, 2013, 34(7): 11-15.

[7] Du ZH, Zhu JQ, Wei T, Huang QS, Zhu XX. Research progress of electrical impedance spectrum technology in the diagnosis of thyroid carcinoma. Chin Base Clin General Surg, 2013, 20(9): 995-1000.

[8] Gao J, Wang BS, Mao JF, Ingvarsson P, Zeng QY, Wang XR. Demography and speciation history of the homoploid hybrid pine Pinus densata on the Tibetan Plateau. Mol Ecol, 2012, 21(19): 4811-4827.

[9] Guo WC, Guo KQ, Zhu XH. Application of dielectric properties in identifying species of tomatoes and apples. Trans Chin Soc Agric Mach, 2006, 37(8): 130-132.

[10] Hao Z, Zhang G, Ya L. Physiological and biochemical indexes and electrical impedance spectroscopy of platyhylla seedings under heat stress. Acta Bot Boreal Occident Sin, 2010, 30(9): 1844-1851.

[11] Li YQ, Zhang G, Que SP, Zhu L, Di B, Jin XM. Relation between electrical impedance spectroscopy parameters and frost hardiness in stems and needles of Pinus bungeana. Scientia silvae Sinicae, 2008, 44(4): 28-34.

[12] Liang D, Mao JF, Zhao W, Zhou XQ, Yuan HW, Wang LM, Xing FQ, Wang XR, Li Y. Seedling performance of Pinus densata and its parental population in the habitat of P. tabuliformis. Chinese. J Plant Ecol, 2013, 37(2): 150-163.

[13] Liu X, Wang G, Zhang G. The relationship between electrical impedance spectroscopy parameters and physiological parameters of wheat leaves under drought stress. Acta Bot Boreal Occident Sin, 2007, 27(5): 859-863.

[14] Liu X, Wang Gd, Zhang G. Response of wheat leaves to electrical impedance spectroscopy parameters in water stress. J Lanzhou Univ (Natural Sciences), 2007, 43(5): 48-53.

[15] Liu YL, Wang XR, Li Y. Geographic isolation between the homoploid hybrid Pinus densata and its parental Pinus yunnanensis. Plant Divers Res, 2011, 33(3): 269-274.

[16] Liu YC, Xiao JZ, Yang JS. Effect of high temperature stress on electrical impedance spectroscopy parameters of chrysanthemum. J Wuhan Bot Res, 2012, 30(2): 198-203.

[17] Luo C-Y, Zhang Z-B, Wang J, He W. Electrical impedance technology applied to examining brain edema. J Chongqing Univ, 2005, 28(2): 32-35.

[18] Ma F, Zhao C, Milne R, Ji M, Chen L, Liu J. Enhanced drought-tolerance in the homoploid hybrid species Pinus densata: Implication for its habitat divergence from two progenitors. New Phytol, 2010, 185(1): 204-216.

[19] MacDougall RG, Thompson R, Piene H. Stem electrical capacitance and resistance measurements as related to total foliar biomass of balsam fir trees. Can J Forest Res, 1987, 17(9): 1071-1074.

[20] Mao JF, Li Y, Liu YJ, Liu H, Wang XR. Cone and seed characteristics of Pinus densata and their adaptive fitness implications. J Plant Ecol (Chinese Version), 2007, 31(2): 291-299.

[21] Meng Y, Di B, Zhang G, Feng XG, Xu CL, Tian J. The correlation analysis of soluble sugar and starch contents with electrical impedance in Betula platyphylla Suk. Roots under waterlogging and flooding stresses. Acta Biophysica Sinica, 2013, 29(6): 450-460.

[22] Repo T, Zhang G, Ryyppö A, Rikala R. The electrical impedance spectroscopy of Scots Pine (Pinus Sylvestris L.) shoots in relation to cold acclimation. J Exp Bot, 2000, 51(353): 2095-2107.

[23] Song BH, Wang XQ, Wang XR, Ding KY, Hong DY. Cytoplasmic composition in Pinus densata and population establishment of the diploid hybrid pine. Mol Ecol, 2003, 12(11): 2995-3001.

[24] Tang Y, Zhang JP. Identification of Kiwifruit and peach varieties based on dielectric propert. J Food Sci, 2012, 33(3): 1-4.

[25] Wang XR, Szmidt AE, Savolainen O. Genetic composition and diploid hybrid speciation of a high mountain pine, Pinus densata, native to the Tibetan Plateau. Genetics, 2001, 159(1): 337-346.

[26] Wang BS, Mao JF, Gao J, Zhao W, Wang XR. Colonization of the Tibetan Plateau by the homoploid hybrid pine Pinus densata. Mol Ecol, 2011, 20(18): 3796-3811.

[27] Xing FQ, Mao JF, Meng JX, Dai JF, Zhao W, Liu H, Xing Z, Zhang H, Wang XR, Li Y. Needle morphological evidence of the homoploid hybrid origin of Pinus densata based on analysis of artificial hybrids and the putative parents, Pinus tabuliformis and Pinus yunnanensis. Ecol Evol, 2014, 4(10): 1890-1902.

[28] Yao L, Zhang G, Lv W, Deng YC, An ZJ, Zhou J. Changes of electrical impedance spectroscopy parameters and electrolyte leakage in different ornamental tree species living in saline. Chin Agric Sci Bull, 2011, 27(6): 54-59.

[29] Zhang G, Xiao JZ, Chen DF. Electrical impedance spectroscopy method for measuring cold hardiness of plants. J Plant Physiol Mol Biol, 2005, 31(1): 19-26.

[30] Zhang J, Zhao HJ, Zhang G, Yang MS. Cold hardiness assessment of germplasm resources in Robinia pseudoacacia using electrical impedance spectroscopy. J Plant Genet Res, 2009, 10(3): 419-425.

[31] Zhou L, Liu L, Wang L, Liu Q. Wang JX, Chang L. Bioelectrical impedance technique and the estimation of postmortem interval. The international symposium on evidence law and forensic science. 2009, Beijing: China University of Political Science and Law Publisher, 286 295