Physiological responses of needles of Pinus massoniana elite families to phosphorus stress in acid soil

Abstract

Pinus massoniana Lamb. is a major timber species widely planted in the South China, where the soil is acidic and deficient in phosphorus (P) due to fixation by aluminum and iron. Understanding the physiological responses to rhizospheric insoluble P is essential for enhancing plantation productivity. Thus, a sand culture experiment was conducted with four levels of P treatment (0, 5, 20 g insoluble P and 10 g soluble P), and 11 P. massoniana elite families. Physiological responses were measured after two months of stress. Compared to the normal soluble P treatment, the insoluble P treatment significantly reduced the proline content and the APase activity in the needles, while it significantly increased the catalase activity by 1.3-fold and malondialdehyde content by 1.2-fold. Soluble protein content was unaffected by the treatments, but chlorophyll content was significantly lower in P-deprived treatment compared with soluble and insoluble P treatments. These physiological responses also exhibited highly significant variation among families (p < 0.01). The findings suggest that increased catalase activities in the presence of insoluble P might be involved in the activation of an anti-oxidation defense mechanism that scavenges the reactive oxygen species elicited by the stress. And this response has a strong genetic control that can be exploited to identify desirable genotypes.

Keywords

Masson pine / phosphorus stress / anti-oxidative system / lipid peroxidation

References

[1] Ao JJ, Kang ZL, Yu Y. Plant physiology experimental techniques. 2007, Beijing: Chemical Industry Press, 133 134

[2] Ashraf M, Foolad MR. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 2007, 59: 206-216.

[3] Barceló J, Poschenrieder C. Fast root growth responses, root exudates, and internal detoxification as clues to the mechanisms of aluminum toxicity and resistance: a review. Environmental and Experimental Botany, 2002, 48: 75-92.

[4] Basu U, Good AG, Taylor GJ. Transgenic Brassica napus plants over-expressing aluminum-induced mitochondrial manganese superoxide dismutase cDNA are resistant to aluminum. Plant Cell Environment, 2001, 24: 1269-1279.

[5] Chen HJ, Li YQ, Chen DD, Zhang Y, Wu LM, Ji JS. Soil phosphorus fractions and their availability in Chinese fir plantations in south China. Forestry Research, 1996, 9: 121-126.

[6] Foyer CH, Noctor G. Oxidant and antioxidant signaling in plants: a reevaluation of the concept of oxidative stress in a physiological context. Plant Cell Environment, 2005, 28: 1056-1071.

[7] Gao Y, Wu X, Sun M. Effects of ectomycorrhizal seedlings of masson pine on absorption and utilization of N, P and K. Journal of Nanjing Forestry University, 2009, 33(4): 33-36.

[8] Gaume A, Mächler F, De Léon C, Narro L, Frossard E. Low-P tolerance by maize (Zea mays L.) genotypes: Significance of root growth, and organic acids and acid phosphatase root exudation. Plant and Soil, 2001, 228: 253-264.

[9] Giannakoula A, Moustakas M, Syrosb T, Yupsanisb T. Aluminum stress induces up-regulation of an efficient antioxidant system in the Altolerant maize line but not in the Al-sensitive line. Environmental and Experimental Botany, 2011, 67: 487-494.

[10] Kochian LV, Hoekenga OA, Piñeros MA. How do crop plants tolerate acid soils?—Mechanisms of aluminum tolerance and phosphorous efficiency. Annual Review of Plant Biology, 2004, 55: 459-493.

[11] Li SF, Liu ST, Zhou JP. Measurement of catalase vigor in plants with spectrophotometry. Anhui Agricultural Science Bulletin, 2007, 13(2): 72-73.

[12] Mao DR. Plant nutrition research methods. 2005, Beijing: China Agricultural University Press, 20 55

[13] Mclachlan KD. Acid phosphatase activity of intact roots and phosphorus nutrition in plants (I): Assay conditions and phosphatase activity. Australian Journal of Agricultural Research, 1980, 31: 42-440.

[14] Ogawa T, Matsumoto C, Takenaka C, Tezuka T. Effect of Ca on Alinduced activation of antioxidant enzymes in the needles of hinoki cypress (Chamaecyparis obtusa). Journal of Forest Research, 2000, 5: 81-85.

[15] Poschenrieder C, Gunsé B, Corrales I, Barceló J. A glance into aluminum toxicity and resistance in plants. Science of the total environment, 2009, 400: 356-369.

[16] Raghothama KG, Karthikeyan AS. Phosphate acquisition. Plant and Soil, 2005, 274: 37-49.

[17] Raghothama KG. Phosphate acquisition. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 50(1): 665-693.

[18] Schachtman DP, Reid RJ, Ayling SM. Phosphorus uptake by plants: from soil to cell. Plant Physiology, 1999, 116: 447-453.

[19] Sharma SS, Dietz KJ. The significance of amino acids and amino acid derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany, 2006, 57: 711-726.

[20] Shi J, Gu Y, Zhao Q. Research on the mycorrhiza fungi of Pinus massoniana Lamb. Evergreen broad-leaved forests in Tainting, Zhejiang province. Journal of Central China University, 2006, 26(1): 41-44.

[21] Singh D, Sale PWG, Pallaghy CK, Singh V. Role of proline and leaf expansion rate in the recovery of stressed white clover leaves with increased phosphorus concentration. New Phytologist, 2000, 146: 26-269.

[22] Smith FW, Jackson WA, Vanden Berg PJ. Internal phosphorus flows during development of phosphorus stress in Stylosanthes hamata. Australian Journal of Plant Physiology, 1990, 17: 451-464.

[23] Snapp S, Lynch JP. Phosphorus distribution and remobilization in bean plants as influenced by phosphorus nutrition. Crop Science, 1996, 36: 929-935.

[24] Vance CP, Uhde-Stone C, Allan DL. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 2003, 157: 423-447.

[25] Veljovic-Jovanovic S, Kukavica B, Stevanovic B, Navari-Izzo F. Senescence and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. Journal of Experimental Botany, 2006, 57: 1759-1769.

[26] Vincent JB, Crowder MW, Averill BA. Hydrolysis of phosphate monoesters: a biological problem with multiple chemical solutions. Trends in Biochemical Science, 1992, 17: 105-110.

[27] Wang J, Han XR, Zhan XM, Hou YH, Zhao WL. Effect of lowphosphorus stress on membrane lipid peroxidation and the protection of enzyme activities in tomato leaves. Plant Nutrition and Fertilizer Science, 2005, 11(6): 851-854.

[28] Wang Y, Ding G. Effects of exogenous mycorrhiza on growth of Pinus massoniana seedlings. Journal of Central South University of Forestry and Technology, 2011, 31(4): 31-34.

[29] Xie YR, Jin GQ, Chen Y, Song ZY. Study on phosphorus efficiency of different provenances of Pinus massoniana. Scientia Silver Sinicae, 2005, 41(4): 25-30.

[30] Xie YR, Zhou ZC. Research advance on adaptation mechanism of forest tree to low-phosphorus stress and genetics of phosphorus efficiency. Forest Research, 2002, 15(6): 734-740.

[31] Xu XH, Ding GJ. Physiological and biochemical responses of Pinus massoniana to low phosphorus stress. Scientia Silver Sinicae, 2006, 42(9): 24-29.

[32] Yan X, Liao H, Trull MC, Beebe SE, Lynch JP. Induction of a major leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean. Plant Physiology, 2001, 125: 1901-1911.

[33] Yoshiba Y, Kiyosue T, Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K. Regulation of levels of proline as an osmolyte in plants under water stress. Plant Cell and Physiology, 1997, 38: 1095-1102.

[34] Yu YC, Yu J, Fang L, Shan Q, Jiang DF. Organic acids exudation from the roots of Cunninghamia lanceolata and Pinus massoniana seedlings under low phosphorus stress. Journal of Nanjing Forestry University, 2007, 31(2): 9-12.

[35] Zhang ZX. Determination of chlorophyll content of plants — acetone and ethanol mixture method. Liaoning Agricultural Science, 1986, 3: 26-29.

[36] Zheng BS. Plant physiology and biochemistry of modern research techniques. Meteorological Press, 2006 107 109

[37] Zhou ZC, Jin GQ, Chen Y. Kinetics of phosphorus uptake by different provenances of Masson Pine under low phosphorus stress. Forest Research, 2003, 16(5): 548-553.

[38] Zhou ZC, Liao GH, Jin GQ, Chen Y. Difference of induced acid phosphate activity under low phosphorus stress of Pinus massoniana provenances. Scientia Silver Sinicae, 2005, 41(3): 58-62.

[39] Zhou ZC, Xie YR, Jin GQ, Wang YS, Hong GM. Inheritance and variation of phosphorus efficiency and its related traits in families of Pinus massoniana. Journal of Beijing Forestry University, 2004, 26(6): 1-5.