Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification

Abstract

Mooseer (Allium hirtifolium Boiss.) is bulbous perennial herb widely used in pharmaceutical and food industry in Iran. We studied germination of mooseer seeds in two separate experiments. In the first experiment, we evaluated four treatments: sulfuric acid scarification, sandpaper scarification, cold stratification, and gibberellic acid (GA₃) application. In the second experiment, we evaluated combinations of these treatments. All treatments in the first experiment had no effect on seed germination, suggesting that mooseer seeds have physical and physiological dormancy. In the second experiment, the highest germination percentage (86.6%) was observed after five minutes scarification with sulfuric acid (75% v/v), followed by 60 days of cold stratification. Duration of sulfuric acid scarification (5, 10, and 20 min) did not affect germination rates, but increasing duration of cold stratification (from 15 to 60 days), increased germination from 28.3% to 86.6%. Our study showed that mooseer seeds have both physical and physiological dormancy.

Keywords

physical dormancy / physiological dormancy / germination / sulfuric acid / sand paper / Allium hirtifolium

References

[1] Baskin C.C., Milberg P., Andersson L., Baskin J.M.. Deep complex morphophysiological dormancy in seeds of Anthriscus sylvestris (Apiaceae). Flora, 2000, 195: 245-251.

[2] Chisha-kasumu E., Woodward S., Price A.. Comparison of the effect of mechanical scarification and gibberellic acid treatments on seed germination in Pterocarpus angolensis. Southern Hemisphere Forestry Journal, 2007, 69: 63-70.

[3] Chuanren D., Bochu W., Wanqian L., Jing C., Jie L., Huan Z.. Effect of chemical and physical factors to improve the germination rate of Echinacea angustifolia seeds. Colloids and Surfaces B: Biointerfaces, 2004, 37: 101-105.

[4] De Villiers A.J., Van Rooyen M.W., Theron G.K.. Germination strategies of strandveld succulent karoo plant species for revegetation purpose: II. dormancy-breaking treatments. Seed Science and Technology, 2002, 30: 35-49.

[5] Ebrahimi R., Zamani Z., Kashi A.. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Scientia Horticulturae, 2009, 119: 345-351.

[6] Ghodrati Azadi H., Ghaffari S.M., Riazi G.H., Ahmadian S., Vahedi F.. Antiproliferative activity of chloroformic extract of Persian shallot, Allium hirtifolium, on tumor cell lines. Cytotechnology, 2008, 56: 179-185.

[7] Hermansen L.A., Duryea M.L., White T.L.. Variability in seed coat dormancy in Dimorphandra mollis. Seed Science and Technology, 2000, 28: 567-580.

[8] Hidayati S.N., Baskin J.M., Baskin C.C.. Dormancy-breaking and germination requirements for seed of Symphoricarpos orbiculatus (Caprifoliaceae). American Journal of Botany, 2001, 88: 1444-1451.

[9] Li P., Wu H., Geng S., Wang X., Lu W., Yang Y., Shultz L.M., Tang T., Zhang N.. Germination and dormancy of seeds in Echinaceae purpurrea(L.) Moench (Asteraceae). Seed Science and Technology, 2007, 35: 9-20.

[10] Malavasi U.C., Malavasi M.M.. Dormancy breaking and Germination of Enterolobium contortisiliquum (Vell.) Morong seed. Brazilian Archives of Biology and Technology, 2004, 47: 851-854.

[11] Nadjafi F., Bannayan M., Tabrizi L., Rastgoo M.. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. Journal of Arid Environments, 2006, 64: 542-547.

[12] Olmez Z., Gokturk A., Temel F.. Effect of cold stratification, sulfuric acid, submersion in hot and top water pretreatment on germination of bladder-senna (Colutea armena Boiss & Huet.) seeds. Seed Science and Technology, 2007, 35: 266-271.

[13] Ortega Baes P., De Viana M.L., Suhring S.. Germination in Prosopis ferox seeds: effect of mechanical, chemical and biological scarification. Journal of Arid Environments, 2002, 50: 185-189.

[14] Patane C., Gresta F.. Germination of Astragalus hamosus and Medicago orbicularis as affected by seed-coat dormancy breaking techniques. Journal of Arid Environments, 2006, 67: 165-173.

[15] Schelin M., Tigabu M., Eriksson I., Sawadogo L., Oden P.C.. Effect of scarification, gibberellic acid and heat treatments on the germination of Balanites aegyptiaca seeds from the Sudanian savanna in Burkina Faso. Seed Science and Technology, 2003, 31: 605-617.

[16] Taran M., Rezaeian M., Izaddoost M.. Invitro antitrichomonas activity of Allium hirtifolium (Persian shallot) in comparison with metronidazole. Iranian Journal Public Health, 2006, 35: 92-94.

[17] Tigabu M., Oden P.C.. Effect of scarification, gibberellic acid and temperature on seed germination of two multipurpose Albizia species from Ethiopia. Seed Science and Technology, 2001, 29: 11-20.

[18] Travlos I.S., Economou G., Karamanos A.I.. Germination and emergence of the hard seed coated Tylosema esculentum (Burch) A. Schreib in response to different pre-sowing seed treatments. Journal of Arid Environments, 2007, 68: 501-507.

[19] Vandelook F., Bolle N., Van Assche J.A.. Morphological and physiological dormancy in seeds of Aegopodium podagraria (Apiaceae) broken successively during cold stratification. Seed Science Research, 2009, 19: 115-123.

[20] Veassey E.A., Teixeira De freitas J.C.. Breaking seed dormancy in Sesbania sesban, Sesbania rostrata and Sesbania virgata. Seed Science and Technology, 2002, 30: 211-217.

[21] Wang Y.R., Hanson J., Mariam Y.W.. Effect of sulfuric acid pretreatment on breaking hardseed dormancy in diverse accessions of five wild Vigna species. Seed Science and Technology, 2007, 35: 550-559.

[22] Zhou Z.Q., Bao W.K., Wu N.. Dormancy and germination in Rosa multibracteata Hemsl. & E. H. Wilson. Scientia Horticulturae, 2009, 119: 434-441.