Augmented growth of long pepper in response to arbuscular mycorrhizal inoculation

Abstract

Arbuscular mycorrhizal (AM) technology is a soil-based fertilization practice for sustainable crop productivity. We evaluated six indigenous Arbuscular mycorrhizal fungi (AMF) strains for their symbiotic response with Piper longum (long pepper), a non-timber forest product holding promise as a commercial crop for its medicinal fruits and roots. Piper saplings were raised in a 10 cm thick sand and soil mix inoculated with various AMF. Under field conditions, plants inoculated with AMF demonstrated better survival (⩽80%) than non mycorrhizal plants (58%). Almost all the studied AMF strains increased the plant growth, biomass and nutrient content (N and P) over the uninoculated control. Mycorrhizal inoculation with four AMF species, viz: Glomus fasciculatum, G. clarum, G. etunicatum and G. versiforme greatly enhanced long pepper growth both in the nursery and field conditions.

Keywords

Piper longum / Arbuscular mycorrhizal fungi / Survival / Growth / non-timber forest product

References

[1] Adivappar N.. Effect of VAM fungi on growth, yield and drought tolerance of Pappaya. 2001, Dharwad, India: University of Agricultural Science

[2] Allen M.F., Allen E.B., Gomez-Pompa A.. Effects of mycorrhizae and non target organisms on restoration of a seasonal tropical forest in Quitana Roo, Mexico: factors limiting tree establishment. Restoration Ecology, 2005, 13(2): 325-333.

[3] Arnon D.I.. Copper enzymes in isolated chloroplasts and polyphenol oxidase in Beta vulgaris. Plant Physiology, 1949, 24(1): 1-15.

[4] Azcón R., Gómez M., Tobar R.. Physiological and nutritional response by Lactuca sativa L. to nitrogen sources and mycorrhizal fungi under drought conditions. Biology and Fertility of soils, 1991, 22(1/2): 156-161.

[5] Azcón R., Rubio R., Barea J.M.. Selective interactions between different species of mycorrhizal fungi and Rhizobium meliloti strains and their effects on growth, N2-fixation (15N), and nutrition of Medicago sativa L. New Phytologist, 1991, 117: 399-404.

[6] Bagyaraj D.J.. Norris J.R., Read D.J., Verma A.K.. Vesicular-arbuscular mycorrhizae application in agriculture. Methods in Microbiology. 1992, London: Academic Press, 359 374

[7] Black C.A.. Methods of soil analysis. 1965, Madison, WI: American Society Agronomy, 1114 1132

[8] Bobby V.U., Bagyaraj D.J.. Biological control of root rots of Coleus forskohlii Briq. Using microbial inoculants. World Journal of Microbiology & Biotechnology, 2003, 19: 175-185.

[9] Bremner J.M., Mulvaney C.S.. Page A.L.. Nitrogen-total. Methods of soil analysis. Part II. 1982, Madison, WI: American Society of Agronomy

[10] Chang D.C.. Robson A.D., Abbot L.K., Malajczuk N.. What is the potential for management of vesicular-arbuscular mycorrhizae in horticulture?. Management of mycorrhizas in agriculture, horticulture and forestry. 1994, Dordrecht, Netherlands: Kluwer Academic Publishers, 187 190

[11] Earanna N.. VA mycorrhizal association in medicinal plants of Southeastern dry zone of Karnataka and response of Phyllanthus amarus and Withania somnifera to inoculation with VAM fungi and plant growth promoting rhizomicroorganisms. 2001, Bangalore: Submitted to University of Agricultural Sciences

[12] Gerdemann J.W., Nicolson T.H.. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Transaction of the British Mycological Society, 1963, 46(2): 235-244.

[13] Gnekow M.A., Marschner H.. Influence of the fungicide pentachloronitrobenzene on VA mycorrhizal and total root length and phosphorous uptake of oats (Avena sativa). Plant and Soil, 1989, 114(1): 91-98.

[14] Gutterman Y.. Seed germination in desert plants. 1993, Berlin and Heidelbeg: Springer-Verlag

[15] Hanson W.C.. The photometric determination of phosphorous in fertilizers using the phosphovanado-molybdate complex. Journal of the Science of Food and Agriculture, 1950, 1(6): 172-173.

[16] Hattingh M.J., Gray L.E., Gerdemann J.W.. Uptake and translocation of Phosphorous-32 labelled phosphate to onion roots by endomycorrhizal fungi. Soil Science, 1973, 116: 383-387.

[17] Jeffries P.. Use of mycorrhizae in agriculture. Critical Review of Biotechnology, 1987, 5(4): 319-357.

[18] Kessel C., Paul W.S., Heinz J.H.. Enhance N transfer from a soybean to Maize by vesicular-arbuscular mycorrhizal fungi. Plant Physiology, 1985, 79: 562-563.

[19] Liu R.J.. Effects of vesicular-arbuscular mycorrhizas and phosphorous on water status and growth of Malus hupehensis. Journal of Plant Nutrition, 1989, 12(8): 997-1019.

[20] Liu R.J., Luo X.S.. Effects of vesicular-arbuscular mycorrhizas on the growth, mineral nutrition and water relations of cherry (Cerasus psedocerasus G. Don). Don, 1988, 5(1): 6-13.

[21] McLean C., Lawrie A.C., Blaze K.L.. The effect of soil microflora on the survival of cuttings of Epacris impressa. Plant and soil, 1994, 166(2): 295-297.

[22] Megan H., Lloyd W.M., Patrick J.B.. Mycorrhizal influence on the nutrition of Strawberries. Journal of American Society for Horticultural Science, 1978, 103: 179-181.

[23] Morton J.B., Bentivenga S.P., Wheeler W.W.. Germplasm in the international collection of arbuscular and vesicular-arbuscular mycorrhizal fungi (INVAM) and procedures for culture development, documentation and storage. Mycotaxon, 1993, 48: 491-528.

[24] Mridha M.A.U., Xu H.L.. Nature Farming with Vesicular-Arbuscular Mycorrhizae in Bangladesh. Journal of Crop Production, 2001, 3(1): 303-312.

[25] Nisha M.C., Rajeshkumar S.. Effect of arbuscular mycorrhizal fungi on growth and nutrition of Wedilia chinensis (Osbeck) Merril. Indian Journal of Science and Technology, 2010, 3(6): 676-678.

[26] Porter W.M.. The most probable number method for enumerating infective propagules of vesicular-arbuscular mycorrhizal fungi in soil. Australian Journal of Soil Research, 1979, 17(3): 515-519.

[27] Rajan S.K., Reddy B.J.D., Bagyaraj D.J.. Screening of arbuscular fungi for their symbiotic efficiency with Tectona grandis. Forest Ecology and Management, 2000, 126(2): 91-95.

[28] Richmond A.E., Lang A.. Effect of Kinetin on protein content and survival of detached Xanthium leaves. Science, 1957, 125(3249): 650-651.

[29] Sailo G.L., Bagyaraj D.J.. Influence of different AM fungi on the growth, nutrition and forskolin content of Coleus forskohlii. Mycological Research, 2005, 109(7): 795-798.

[30] Schenck N.C., Perez Y.. Manual for the identification of VA mycorrhizal fungi, 3rd eds. 1990, Gainesville, Florida: Synergistic Publications

[31] Shivaputra S.S., Patil C.P., Swamy S.K., Patil P.B.. Cumulative effect of VAM fungi and vermicompost on nitrogen, phosphorous, potassium and chlorophyll content of Papaya leaf. Mycorrhiza News, 2004, 16(2): 15-16.

[32] Sivarajan V.V., Balachandran I.. Ayurvedic Drugs and their Plant Sources. 1996, New Delhi: Mohan Primalini for Oxford and IBH Publishing Company, PVT. LTD, 374 376

[33] Strack D., Fester T., Hause B., Schliemann W., Walter M.H.. Arbuscular mycorrhiza: biological, chemical and molecular aspects. Journal of Chemical Ecology, 2003, 29(9): 1955-1979.

[34] Stutz J.C., Cooperman R., Martin C.A., Morton J.B.. Patterns of species composition and distribution of arbuscular mycorrhizal fungi in arid regions of southwestern North America and Namibia, Africa. Canadian Journal of Botany, 2000, 78: 237-245.

[35] Talukdar N.C., Germida J.J.. Propagation and storage of vesicular-arbuscular mycorrhizal fungi isolated from Saskatchewan agricultural soils. Canadian Journal of Botany, 1993, 71: 1328-1335.

[36] Vasanthakrishna M., Bagyaraj D.J., Nirmalnath J.P.. Selection of efficient VA mycorrhizal fungi for Casuarina equisetifolia — Second screening. New Forests, 1995, 9(2): 157-162.

[37] Viswanathan TV. 1995. Medicinal and Aromatic Plants, In: Chadha KL, Gupta Rajendra (eds), Advances in Horticulture., vol. 1, pp. 373–383

[38] Zhu Y.G., Laidlaw A.S., Christie P., Hammond M.E.R.. The specificity of arbuscular mycorrhizal fungi in perennial ryegrass-white clover pasture. Agriculture, Ecosystems & Environment, 2000, 77: 211-218.