Extraction, purification and antioxidant activity of polysaccharides from bamboo leaves

Abstract

Ultrasonic extraction (UE) was employed for the extraction of bamboo leaf polysaccharides (BLP). The influential parameters of UE procedure including extraction time, ultrasonic power and solid/liquid ratio were optimized by orthogonal experiments. DEAE-cellulose column chromatography was applied to purify BLP and then the radical scavenging activity of BLP was also evaluated. Optimal extraction conditions were: extraction time of 15 min, ultrasonic power of 300 W, and solid/liquid ratio of 1:15. Four kinds of polysaccharides were obtained by DEAE-cellulose column chromatography; the maximum superoxide radical scavenging rate (20.4%) of BLP was inferior to that of vitamin C (V_C, the control) and the hydroxyl radical scavenging rate (50%) was equivalent to that of V_C.

Keywords

bamboo leaves / polysaccharide / scavenging radical / separation and purification / ultrasonic extraction