Dynamic changes of phenolic compound contents in leaf and bark of poplar during autumn temperature drop

Abstract

We investigated seasonal dynamics of phenolics substance in leaf and bark of two cultivars of poplar, Populus alba×berolinensis and P. בZhonglin Sanbei 1’, during autumn temperature drop for analyzing the roles of phenolic secondary metabolites in cold resistance. Results show that the contents of condensed tannin and flavonoid in poplar leaf and the flavonoid contents in bark of P. בZhonglin Sanbei 1’ were increased with the decrease of autumn minimum temperature, showing a significantly negative correlation between the contents of soluble phenolic substance in the leaves and changeable temperature. In contrast, lignin content in the poplar leaves is decreased in the process of temperature drop, showing a significantly positive correlation. These results indicate that the variation in phenolic substance has a close correlation with its cold-resistance during the autumn temperature drop.

References

[1] Agrawal A.A.. Induced responses to herbivory and increased plant performance. Science, 1998, 279: 1201-1202.

[2] Butler L.G., Price M.L., Brotherton J.E.. Vanillin assay for proanthocyanidins (condensed tannins): modification of the solvent for estimation of the degree of polymerization. J Agric Food Chem, 1982, 30(6): 1087-1098.

[3] Chen X., Ye Hechun.. Li J.. Plant secondary metabolism and its regulation. Plant Science Advance. 1998, Beijing: Higher Education Press, 293 394

[4] Dixon R.A., Ferreira D.. Molecules of interest genistein. Phytochemistry, 2002, 60: 205-211.

[5] Fei Y., Shu N., Huang T., Zhao Shuhui.. Cellular chemistry and biochemistry of plant cold-injure and -resistance. Progress in Biotechnology, 1994, 14: 40-44.

[6] Gao D., Wang Y., Yu Wenxi.. Responses of phenolic compounds in poplar leaves to abrupt temperature decrease in autumn. Journal of Northeast Forestry University, 2009, 37(5): 4-6.

[7] Keinanen M., Julkunen-Tiitto R., Hoshino T., Odaira M., Yoshida M., Tsuda S.. Physiology and biochemical significance of antifreeze substances in plants. Journal of Plant Research, 1999, 112: 255-261.

[8] Gershenzon J.. Changes in the leaves of plant secondary metabolites under water and nutrient stress. Recent Advance sin Phytochemistry, 1983, 18: 273-320.

[9] Li Chunming.. Studies on seasonal dynamics of phenolic compounds in poplar. 2007, Harbin, China: Northeast Forestry University

[10] Li X., Wang J., Pang Z., Zhao X., Li Yongcun.. Evaluation of Growth and Management Density for Yingchun Poplar 5. Journal of Northeast Forestry University, 1998, 26(6): 24-26.

[11] Li P.H., Palva E.T.. Plant cold hardiness. 2002, New York: Kluwer Academic/plenum Publishers

[12] Li P.H., Sakai A.. Plant cold hardiness and freezing stress Vol II. 1982, New York: Academic Press

[13] Li W., Xiong J., Chen Xiaoyang.. Advances in the research of physiological significances and genetic regulation of lignin metabolism. Acta Botanica Boreali-occidentalia Sinica, 2003, 23(4): 675-681.

[14] Lindroth R.L., Hwang S.Y.. Clonal variation in foliar chemistry of quaking aspen (Populus tremuloides Michx.). Biochemical Systematics and Ecology, 1996, 24: 357-364.

[15] Lindroth R.L., Osier T.L., Barnhill H.R.H., Wood S.A.. Effects of genotype and nutrient availability on phytochemistry of trembling aspen (Populus tremuloides Michx.) during leaf senescence. Biochemical Systematics and Ecology, 2002, 30: 297-307.

[16] Massei G., Hartley S.E., Bacon P.J.. Chemical and morphological variation of Mediterranean Wiidy Evergreen Species: Do plants respond to ungulate browsing?. Journal of Vegetation Science, 2000, 11(1): 1-8.

[17] Morimoto M., Tanimoto K., Sakatani A., Komai K.. Antifeedant activity of an anthraquinone aldehyde in Galium aparine L. against Spodoptera Litura F. Phytochemistry, 2002, 60(2): 163-166.

[18] Mutikainen P., Walls M., Ovaska J., Vapaavuori. Trade-offs in phenolic metabolism of silver birch: effects of fertilization, defoliation, and genotype. Ecology, 1999, 80: 1970-1986.

[19] Pan Ruizhi.. Plant Physiology. 2001, Beijing: Higher Education Press

[20] Priyanka K., Mishra S.B., Priyanka K.. Biochemical markers for cold tolerance in Boro rice. The Indian Journal of Genetics & plant Breeding, 2001, 61: 23-26.

[21] Shelton A.L.. Variable chemical defences in plants and their effects on herbivore behaviour evolutionary. Ecology Research, 2000, 2: 231-249.

[22] Teklemariam T.A., Blake T.J.. Phenylalanine ammonia-lyase-induced freezing tolerance in jack pine (Pinus banksiana) seedlings treated with low, ambient levels of ultraviolet-B radiation. Physiologia plantarum, 2004, 122: 244-253.

[23] Teklemariam T.A.. Effects of near-ambient ultraviolet-B radiation on seedling tolerance of extreme temperatures. 2002, Toronto: University of Toronto (Canada), 304.

[24] Walton J.D.. Host-selective toxins: agents of compatibility. The Plant Cell, 1996, 8: 1723-1733.

[25] Wei J., Zhao H., Zhang J., Liu H., Song Yanru.. Cloning of cDNA encoding CCoAOMT from Populus tomentosa and down-regulation of lignin content in transgenic plant expressing antisense gene. Acta Botanica Sinica, 2001, 43: 1179-1183.

[26] Xi X., Li Shouxing.. Analysis on contents of flavonoids and polysaccharides in pollen of Typha angustifolia L. and its different processed products. China Journal of Chinese Materia Medica, 2000, 25(1): 25-28.

[27] Zhang J., Zhao Y., Tang Y., et al. Afforestation testing research of different aging Yinzhongyang polar trees. Protection Forest Science and Technology, 1994, 20(3): 27-31.