Genetic diversity and relationship between cultivated clones of Dalbergia sissoo of wide geographical origin using RAPD markers

Abstract

Random Amplified Polymorphic DNA (RAPD) polymorphism was employed to assess the genetic diversity in the elite germplasm of Dalbergia sissoo. Sixty-seven clones that are under cultivation in northern India, originated from six different states of India and Nepal were analyzed with 30 RAPD primers that generated a total of 342 fragments out of which 290 were polymorphic. Total genetic diversity (Ht) varied between 0.01 and 0.37, with an average of 0.19. Shannon’s Information index (I) varied between 0.02 and 0.54, with an average of 0.31. Marker attributes like Polymorphism Information Content (PIC), Marker Index (MI) and Effective Multiplex Ratio (EMR) values were calculated to assess the discriminatory power of 30 primers used. The PIC values ranged from 0.01 to 0.37 with an average of 0.17 per primer and the EMR ranged from 0.17 to 21.00 with a mean of 8.66 across all genotypes. Closely related clones were C49 and C51 with similarity index of 0.86 while the least similar or most dissimilar clones were C14 and S-DB showing similarity index of 0.58. The UPGMA-phenogram categorized the 67 clones into six clusters based on genetic similarity and dissimilarity. The clustering of clones in relation to their geographical location has been discussed.

Keywords

clones / Dalbergia sissoo / genetic diversity / polymorphic / RAPD