Optimal ultrasonic extraction condition and determination of polysaccharides in Tilia amurensis flowers

Abstract

Seven extracting temperatures (25, 35, 45, 55, 65, 75, 85°C) and extracting time (30, 40, 50, 60, 70, 80, 90 min) were designed for selecting the optimal extracting time and extracting temperature for ultrasonic extraction of T. amurensis flowers. Polysaccharides from T. amurensis flowers were isolated and determined by spectrophotometry. Results show that the optimal ultrasonic temperature was 75°C and extracting time 52 min. The content of polysaccharides in T. amurensis flowers measured by anthrone—H₂SO₄ colorimetry under 580 nm, was 9.74% with 0.47% of relative standard deviation (RSD, n=3). This study demonstrated that ultrasonic extraction method was simple, and the color of the treated samples was stable in 4 h. The average recovery value for the polysaccharides measured was 99.48%±1.01%, with 0.112% of RSD (n=3).

Keywords

best extracting condition / polysaccharides / Tilia amurensis flowers / ultrasonic extraction