Variations in seed germination of Hippophae salicifolia with different presoaking treatments

Abstract

Mature seeds of H. salicifolia, collected from five provenances (i.e. Hanuman Chatti, Helang, Lata, Rambara and Janggal Chatti) in west Himalaya, India, were treated with stratification (at 4°C for 15, 30 and 60 days) and in different concentrations of GA₃ (5, 10, 20 mM), KNO₃ (50, 100, 200 mM) and Thiourea (50, 100, 200 mM) solution to determine the variations in seed germination. Results reveal that the germination rates of seeds from different provenances under different pre-sowing treatments are significantly increased compared to those in control (24%–30%). The seeds treated with Thiourea (100 mM) have highest germination rate (76%–83% for different seed sources), followed by those (63%–71% for different seed sources) pretreated with stratification (4°C, 30 days). GA3 treatment significantly shortens the mean germination time (MGT) and improves seed germination percentage. Considering the practical applicability and cost effectiveness, thiourea (100 mM) and stratification (at 4°C) treatments for seed germination are recommended for mass multiplication through seeds of H. salicifolia in village/forest nurseries of the west Himalaya, India.

Keywords

Seed germination percentage / Hippophae salicifolia / presoaking treatment / stratification / Thiourea

References

[1] Anderson L., Milberg P.. Variation in seed dormancy among mother plants, populations and year of seed collection. Seed Science Research, 1998, 8: 29-38.

[2] Baskin C.C., Baskin J.M.. Seed: ecology, biogeography and evolution of dormancy and germination. 1998, California (USA): Academic Press, 668.

[3] Bewley J.D., Black M.. Seed physiology of development and germination. 1994, New York: Plennum Press, 221.

[4] Chaudhary D.K., Kaul B.L., Khan S.. Breaking seed dormancy of Podophyllum hexandrum Royle ex Camb (syn. P. emodi Wall. Ex Honigberger). Journal of Non Timber Forest Products, 1996, 3: 10-12.

[5] Friis I.. Forests and Forest Trees of Northeast Tropical Africa: their natural habitats and distribution patterns in Ethiopia, Djibouti and Somalia. 1992, London: Royal Botanic Gardens, 396.

[6] Gamble J.S.. A Manual of Indian Timbers, 1972 2 Dehradun: Bishen Singh Mahendra Pal Singh, 868.

[7] Gaur R.D.. Flora of the District Garhwal Northwest Himalaya (with ethnobotanical notes). 1999, Srinagar Garhwal, India: Transmedia, 811.

[8] Hartmann H.T., Kester D.E., Davis F.T.. Plant Propagation: Principles and Practices, 1990 5 New Jersey: Prentice-Hall, Englewood Cliffs, 647.

[9] Hendry G.A.F., Price A.H.. Hendry G.A.F., Grime J.P.. Stress indicators: chlorophylls and carotenoids. Methods in Comparative Plant Ecology. 1993, London: Chapman & Hall, 148 152

[10] Hooker J.D.. The Flora of British India. 1894, Dehradun: Bishen Singh Mahendra Pal Singh, 791.

[11] Huxley A.J., Griffiths M., Levy M.. The New Royal Horticultural Society Dictionary of Gardening. 1992, London: Macmillan Reference Limited, 33 34

[12] Khan A.A., Heit C.E.. Selective effects of Hormones on nucleic acid metabolism during germination of pear embryos. Biochemistry Journal, 1969, 113: 707-712.

[13] Mamo N., Mihretu M., Fekadu M., Tigabu M., Teketay D.. Variation in seed and germination characteristics among Juneperus procera populations in Ethiopia. Forest Ecology and Management, 2006, 225: 320-327.

[14] Matthews V.. The New Plantsman. 1994, London: Royal Horticultural Society, 1352 4186

[15] McIntyre G.I., Cessna A.J., Hsiao A.I.. Seed dormancy in Avena fatua: interacting effects of nitrate, water and seed coat injury. Physiologia Plantarum, 1996, 97: 291-302.

[16] Nikolaeva M.G.. Physiology of deep dormancy in Seeds. 1969, Leningrad.: lsdakel’stvo Nauka, 220.

[17] Pandey H., Nandi S.K., Nadeem M., Palni L.M.S.. Chemical stimulation of seed germination in Aconitum heterophyllum Wall. and A. balfourii Stapf.: Important Himalayan species of medicinal value. Seed Science Technol, 2000, 28: 39-48.

[18] Pearson M.C., Rogers J.A.. Hippophae rhamnoides L. Journal of Ecology, 1962, 50: 501-513.

[19] Rawat B.S., Sharma C.M., Ghildiyal S.K.. Improvement of Seed germination in three important conifer species by Glibberellic acid (GA₃). Lyonia, 2006, 11(2): 23-30.

[20] Sankhyan H.P., Sehgal R.N., Bhrot N.P.. Standardization of presowing treatments for different seabuckthorn species in cold deserts of Himachal Pradesh. Indian Forester, 2005, 131(7): 931-938.

[21] Siril E.A., Dhar U., Dhyani P.P.. Seed germination of Chinese Tallow tree (Sapium sebiferum). Forests, Farm and Community Tree Research Reports, 1998, 3: 55-58.

[22] Snedecor G.W., Cochran W.G.. Statistical Methods, 1967 6 Ames: Iowa State University Press, 421 432

[23] Stokes P.. Temperature and seed dormancy. Encyclopaedia of Plant Physiology, 1965, 15: 746-803.

[24] Tewari A., Dhar U.. An investigation on seed germination of Indian Butter tree Aisandra butyracea. Seed Science Technology, 1996, 24: 211-218.

[25] Wilkinson L.. SYSTAT: The system for statistics. 1986, Evanston, Illinois: SYSTAT Inc., 660.