Effects of Thidiazuron, basal medium and light quality on adventitious shoot regeneration from in vitro cultured stem of Populus alba×P. berolinensis

Abstract

The effect of Thidiazuron (TDZ), basal media and light quality on adventitious shoot regeneration from in vitro cultured stem of Populus alba×P. berolinensis were determined to establish a high efficiency shoot regeneration system from stem explants of P. alba×P. berolinensis. Stems of Populus alba×P. berolinensis were collected from cultured shoots in vitro derived from dormancy buds of 3-year-old seedlings. The stem explants were cultured on MS medium containing 0.02-mg·L⁻¹ NAA (naphthaleneacetic acid), and 0.1, 0.3, 0.5 and 1.0 mg·L⁻¹ concentrations of TDZ to determine the effect of TDZ on shoot regeneration. Three basal media, i.e. MS, woody plant medium (WPM) and B5, were used to test their influences of different media on adventitious shoot regeneration. Green, red, blue and yellow plastic films in comparison with florescent light as control were used to observe their effects on shoot regeneration. The results showed that different concentrations of TDZ had an evident influence on shoot regeneration. Lower concentration of TDZ (0.1 mg·L⁻¹) resulted in more adventitious shoot regeneration and higher concentration of TDZ (>0.1 mg·L⁻¹) inhibited shoot regeneration. Among different media, MS medium exhibited a high efficiency for shoot regeneration, followed by WPM medium, while B5 medium inhibited shoot regeneration. Normal light and yellow light exhibited better effects on shoot regeneration, compared with other light.

Keywords

Populus alba×P. berolinensis / stem explants / shoot regeneration

References

[1] Burritt D.J., Leung D.W.M. Adventitious shoot regeneration from Begonia×erythrophylla petiole sections is developmentally sensitive to light quality Physiol. Plant., 2003, 118: 289-296.

[2] Dai W.H., Cheng Z.M., Sargent W. Plant regeneration and Agrobacterium-mediated transformation of two elite aspen hybrid clones from in vitro leaf tissues In vitro Cell Dev. Biol. Plant, 2003, 39: 6-11.

[3] Huetteman C.A., Preece J.E. Thidiazuron: a potent cytokinin for woody plant tissue culture Plant Cell Tiss. Organ Cult., 1993, 33: 105-119.

[4] Ledbetter D.I., Preece J.E. Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants Sci. Horti., 2004, 101: 121-126.

[5] Liu C.Z., Murch S.J., Demerdash M.E.L., Saxena P.K. Regeneration of the Egyptian medicinal plant Artemisia judaica L Plant Cell Rep., 2003, 21: 525-530.

[6] Mithila J., Hall J.C., Victor J.M.R., Saxena P. K. Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha WEndl.) Plant Cell Rep., 2003, 21: 408-414.

[7] Murthy B.N.S., Murch S.J., Saxena P.K. Thidiazuron: a potential regulator of in vitro plant morphogenesis In vitro Cell. Dev. Biol. Plant., 1998, 34: 267-275.

[8] Noël N., Leplé J.C., Pilate G. Optimization of in vitro micropropagation and regeneration for Populus×interamericana and Populus ×euramericana hybrids (P. deltoids, P. trichocarpa, and P. nigra) Plant Cell Rep., 2002, 20: 1150-1155.

[9] Pelah D., Kaushik R.A., Mizrahi Y., Sitrit Y. Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron Plant Cell Tiss, Organ Cult., 2002, 71: 81-84.

[10] Qin Y.H., Zhang S.L., Syed A., Zhang L.X., Qin Q.P., Chen K.S., Xu C.J. Regeneration mechanism of Toyonoka strawberry under different color plastic films Plant Sci., 2005, 168: 1425-1431.

[11] Siril E.A., Dhar U. A highly efficient in vitro regeneration methodology for mature Chinese tallow tree(Sapium sebiferum Roxb.) Plant Cell Rep., 1996, 16: 83-87.

[12] Schween G., Schwenkel H.G. In vitro regeneration in Primula spp. via organogenesis Plant Cell Rep., 2002, 20: 1006-1010.

[13] Thakur A.K., Sharma S., Srivastava D.K. Plant regeneration and genetic transformation studies in petiole tissue of Himalayan poplar (Populus ciliate Wall.) Current Sci., 2005, 89: 664-668.