Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var. mongolica plantations in southeastern Keerqin sandy lands, China

Mei-ling Xu , Jiao-jun Zhu , Hong-zhang Kang , Ai-hua Xu , Jin-xin Zhang , Feng-qin Li

Journal of Forestry Research ›› 2008, Vol. 19 ›› Issue (2) : 113 -118.

PDF
Journal of Forestry Research ›› 2008, Vol. 19 ›› Issue (2) : 113 -118. DOI: 10.1007/s11676-008-0019-2
Research Paper

Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var. mongolica plantations in southeastern Keerqin sandy lands, China

Author information +
History +
PDF

Abstract

The effects of medium, pH, water potential and temperature on the culture for three pure strains (Lactarius deliciosus, Boletus edulis and Lactarius insulsus) of ectomycorrhizal fungi from plantation forests of Mongolian pine (Pinus sylvestris var. mongolica) on sandy lands were observed to obtain the optimum conditions for the growth of ectomycorrhizal fungi. The results indicated that the three ectomycorrhizal fungi could grow well in the mediums containing natural components, such as vitamin, pine juice and yeast powder. pH had a slight effect on the growth of the three ectomycorrhizal fungi, and the optimum pH values were 6.0 for L. deliciosus, 5.0 for B. edulis, respectively. However, L. insulsus had a wide pH range, and it grew better than the other two strains in neutral and light alkalescent mediums. Water potential (produced by Polyethylene Glycol, PEG) had significant effects on the ecological adaptability for the tested three fungi strains. All of the three stains grow better at lower PEG concentration (100 g PEG·kg−1 H2O). The best water potential was 10% PEG concentration for all of the three stains. Temperatures, especially high temperatures induced the fungi death. The optimum temperature for the growth of ectomycorrhizal fungi was 25–28°C for all of the three stains.

Keywords

Mongolian pine (Pinus sylvestris var. mongolica) / ectomycorrhizal fungi / medium / pH / water potential / temperature

Cite this article

Download citation ▾
Mei-ling Xu, Jiao-jun Zhu, Hong-zhang Kang, Ai-hua Xu, Jin-xin Zhang, Feng-qin Li. Optimum conditions for pure culture of major ectomycorrhizal fungi obtained from Pinus sylvestris var. mongolica plantations in southeastern Keerqin sandy lands, China. Journal of Forestry Research, 2008, 19(2): 113-118 DOI:10.1007/s11676-008-0019-2

登录浏览全文

4963

注册一个新账户 忘记密码

References

[1]

Ahonen-Jonnarth U. 2000. Growth, nutrient uptake and ectomycorrhizal function in Pinus sylvestris plants exposed to aluminium and heavy metals. Doctoral Thesis. Swedish University of Agricultural Sciences. SLU Service/ Repro, Uppsala. ISBN 91-576-5864-1.

[2]

Allen M.F. The Ecology of Mycorrhizae, 1991 Cambridge: Cambridge University Press

[3]

Baar J., Comini B., Elferink M.O., Kuyper T.W. Performance of four ectomycorrhizal fungi on organic and inorganic nitrogen sources Mycol Res, 1997, 101: 523-529.

[4]

Bi G., Guo X. Influence of temperature on colony growth of ectomycorrhizal fungi in pure culture For Res, 1989, 3: 247-253.

[5]

Byrd K.B., Thomas Parker V., Vogler D.R., Cullings K.W. The influence of clear-cutting on ectomycorrhizal fungus diversity in a lodgepole pine (Pinus contorta) stand, Yellostone National Park, Wyoming, and Gallatin National Forest, Montana Canadian Journal of Botany, 2000, 78: 149-156.

[6]

Chen G.S., Zeng D.H., Chen F.S. Concentrations of foliar and surface soil in nutrients Pinus spp. Plantations in relation to species and stand age in Zhanggutai sandy land, northeast China Journal of Forestry Research, 2004, 15: 11-18.

[7]

Giltrap N.J., Lewis D.H. Inhibition of growth of ectomycorrhizal fungi in culture by phosphate New Phytol, 1981, 87: 669-675.

[8]

Gregory K., Eaton Plasticity and constraint in growth and protein mineralization of ectomycorrhizal fungi under simulated nitrogen deposition Mycologia, 2002, 94: 921-932.

[9]

Griffin D.M. Kozlowski T. T. Effect of soil moisture on survival and spread of pathogens Water deficits and Plant growth Vol. 5, 1978 New York: Academic Press 175-197.

[10]

Guo X., Bi G. Forest Mycorrhiza and Its Applied Technology, 1989 Beijing: China Forestry Publishing House 43-62.

[11]

Han G., Liu C., Zhou Y. Effect of temperature and pH on mycorrhizal fungus growth Chin J Ecol, 1993, 12: 15-19.

[12]

Hung L., Trappe J.M. Growth variation between and within species of ectomycorrhizal fungi in reaponse to pH in vitro Mycologia, 1983, 75: 234-241.

[13]

Jefferies R.L., Maron J.L. The embarrassment of riches: atmospheric deposition of nitrogen and community and ecosystem processes Trends Ecol Evol, 1997, 12: 74-78.

[14]

Johansson JF. 2002. Belowground ectomycorrhizal community structure along a local nutrient gradient in a boreal forest in Northern Sweden. Swedish University of Africultural Sciences, Uppsala 1–26.

[15]

Jonbloed R.H., Borst-pauwels G.W.F.H. Effect of ammonium and pH on growth of some ectomycorrhizal fungi in vitro Acta Bot Neerl., 1990, 39: 349-358.

[16]

Kaufmann M.R., Eckard A.N. Evaluation of water stress control with polyethylene glycols by analysis of guttation Plant physiol., 1971, 47: 453-456.

[17]

Machado H, Braganca H. 1996. In vitro study of ectomycorrhiza formation under drought stress conditions. In: Mycorrhizas in integrated systems from genes to plant development (Proceedings of the fourth European symposium on mycorrhizas), pp455–458.

[18]

Marx D.H. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria Phytopathology, 1969, 59: 153-163.

[19]

Marx D.H., Daniel W.J. Maintaining cultures of ectocycorrhizal an plant pathogenic fungi in sterile water cold storage Canad J Microbiol, 1976, 22: 338-341.

[20]

Mexa J., Reid C.P.P. The growth of selected mycorrhizal fungi in response to induced water stress Canad J Bot, 1973, 51: 1579-1588.

[21]

Michel B.E. Further comparisons between Carbowax 6000 and mannitol as suppressants of cucumber hypocotyls elongation Plant physiol, 1971, 48: 513-516.

[22]

Nazar R.N., Robb E.J., Volossiouk T. Akkermans A. D. L., van Elsas J. D., de Bruijn) F.J. Direct extraction of fungal DNA from soil Molecular Microbial Ecology Manual 1.3.6, 1996 Kluwer: Dordrecht 1-8.

[23]

Sánchez F., Honrubia M., Torres P. Effects of pH, water stress and temperature on in vitro cultures of ectomycorrhizal fungi from Mediterranean forests Cryptogamie Mycol, 2001, 22: 243-258.

[24]

Smith S.E., Read D.J. Mycorrhizal symbiosis, 1997 2nd edition New York: Academic Press 605

[25]

Theodorou C. Influence of temperature on the mycorrhizal association of Pinus rodiota Aust J Bot, 1971, 19: 13-20.

[26]

Wang H., Dai L.M., Yang B.S., Lang Q.L., Gu H.Y. Occurrence and culture of mycorrhizal fungi associated with oaks in Dandong region, Liaoning province Pedosphere, 2005, 15: 232-237.

[27]

Wu B. Mycorrhiza and water stress J Beijing For Univ, 1991, 13: 95-104.

[28]

Wu X., Liu G., Han H. Soil quality in the different types of Pinus sylvestris var. mongolica man-made sand-fixation forest J Beihua Univ (Natural Science), 2002, 3: 76-79.

[29]

Xu M., Zhu J., Sun J., Kang H., Xu H., Zhang H. A review on the relationships between forest ectomycorrhizal fungi and environmental factors Chin J Ecol, 2004, 23: 212-217.

[30]

Zhao Z., Guo X. Ecological studies on ectomycorrhizal fungi in pure cultures For Res, 1989, 2: 136-141.

[31]

Zhang Z., Zheng W., Yi K., Li J., Chen X. A study on ecology of Russula Vinose Acta Ecol Sinica., 1996, 16: 208-210.

[32]

Zhou C., Han G., Zhou Y., Liu C., Zhang W., Xu G. The research of some ectomycorrhizal fungi of pine Acta Ecol Sinica, 1983, 2: 103-109.

[33]

Zhu J., Xu H., Xu M., Kang H. Review on the ecological relationships between forest trees and ectomycorrhizal fungi Chin J Ecol, 2003, 22: 70-76.

[34]

Zhu J.J., Fan Z.P., Zeng D.H., Matsuzaki T. Comparison of stand structure and growth between plantation and natural forests of Pinus sylvestris var. mongolica on sandy land Journal of Forestry Research, 2003, 14: 103-111.

[35]

Zhu J., Li Z., Kang H. Effect of polyethylene glycol(PEG)-simulated drought stress on Pinus sylvestris var. mongolica seed germination on sandy land Chin J Appl Ecol, 2005, 16: 801-804.

AI Summary AI Mindmap
PDF

142

Accesses

0

Citation

Detail

Sections
Recommended

AI思维导图

/