Effects of freeze-thaw on soil nitrogen and phosphorus availability at the Keerqin Sandy Lands, China

Abstract

A laboratory simulated freeze-thaw was conducted to determine the effects of freeze-thaw on soil nutrient availability in temperate semi-arid regions. Soil samples were collected from sandy soils (0–20 cm) of three typical ecosystems (grassland, Mongolian pine plantation and poplar plantation) in southeastern Keerqin Sandy Lands of China and subjected to freeze-thaw treatment (−12°C for 10 days, then 20°C for 10 days) or incubated at constant temperature (20°C for 20 days). Concentrations of the soil NO₃ ⁻-N, NH₄ ⁺-N, NaHCO₃ extractable inorganic P (LPi) and microbial biomass P (MBP) were determined on three occasions: at the start of the incubation, immediate post-thawing and at the 10th day post-thawing. The results showed that soil net nitrification and N mineralization rates at three sites were negatively affected by freeze-thaw treatment, and decreased by 50%–85% as compared to the control, of which the greatest decline occurred in the soil collected from poplar plantation. In contrast, the concentration of soil NH₄ ⁺-N, NaHCO₃ extractable inorganic P (LPi) and microbial biomass P were insignificantly influenced by freeze-thaw except that LPi and NH₄ ⁺-N showed a slight increase immediate post-thawing. The effects of freeze-thaw on soil N transformation were related to soil biological processes and the relatively constant available P was ascribed to severe soil aridity.

Keywords

freeze-thaw / temperate semi-arid region / nitrification / phosphorus availability

References

[1] Bowman R.A., Cole C.V. An exploratory method for fractionation of organic phosphorus from grassland soils Soil Sci, 1978, 125(2): 95-101.

[2] Bramley R.G.V., Barrow N.J., Shaw T.C. The reaction between phosphate and dry soil. I. The effect of time, temperature and dryness J Soil Sci, 1992, 43(4): 749-758.

[3] Brookes P.C., Powlson D.S., Jenkinson D.S. Measurement of microbial biomass phosphorus in soil Soil Biol Biochem, 1982, 14(4): 319-329.

[4] Chen F.S., Zeng D.H., Zhou B., Singh A.N., Fan Z.P. Seasonal variation in soil nitrogen availability under the Mongolian pine plantations at the Keerqin Sand Lands, China J Arid Environ, 2006, 67(2): 226-239.

[5] Deluca T.H., Keeney D.R., McCarty G.W. Effects of freeze-thaw events on mineralization of soil nitrogen Biol Fertil Soils, 1992, 14: 116-120.

[6] Edwards A.C., Cresser M.S. Freezing and its effect on chemical and biological properties of soil Adv Soil Sci, 1992, 18: 59-79.

[7] Freppaz M., Williams B.L., Edwards A.C., Scalenghe R., Zanini E. Simulating soil freeze/thaw cycles typical of winter alpine conditions: Implication for N and P availability Appl Soil Ecol, 2007, 35: 247-255.

[8] Groffman P.M., Driscoll C.T., Fahey T.J. et al. Colder soils in a warmer world: A snow manipulation study in a northern hardwood forest ecosystem Biogeochemistry, 2001, 56(2): 135-150.

[9] Henry H.A.L. Soil freeze-thaw cycle experiments: Trends, methodological weaknesses and suggested improvements Soil Biol Biochem, 2007, 39(5): 977-986.

[10] Hinman W.G. Effects of freezing and thawing on some chemical properties of three soils Can J Soil Sci, 1970, 50: 179-182.

[11] Liu G. Standard methods for the observation and analysis of Chinese ecosystem research network: soil analysis and profile description, 1996 Beijing: Standard Press of China

[12] Liu M., Su F., Ma D., Wu X., Sun H. Decline reasons of pure Pinus sylvestris var. mongolica forests and soil fertility J Shenyang Agri Univ, 2002, 33(4): 274-277.

[13] Murdoch P.S., Stoddard J.L. Chemical characteristics and temporal trends in eight streams of the Catskill Mountains, New York Water Air Soil Poll, 1993, 67: 367-395.

[14] Neilsen C.B., Groffman P.M., Hamburg S.P., Driscoll C.T., Fahey T.J., Hardy J.P. Freezing effects on carbon and nitrogen cycling in Northern Hardwood forest soils Soil Sci Soc Am J, 2001, 65: 1723-1730.

[15] Peltovuori T., Soinne H. Phosphorus solubility and sorption in frozen, air-dried and field-moist soil Euro J Soil Sci, 2005, 56(6): 821-826.

[16] Read D.W.L., Cameron D.R. Changes in the amount of nitrogen and phosphorus in the soil between the fall and spring sampling Can J Soil Sci, 1979, 59(6): 271-276.

[17] Ron Vaz M.D., Edwards A.C., Shand C.A., Cresser M.S. Changes in the chemistry of soil solution and acetic-acid extractable P following different types of freeze/thaw episodes Euro J Soil Sci, 1994, 45: 353-359.

[18] Schimel J.P., Clein J.S. Microbial response to freeze-thaw cycles in tundra and taiga soils Soil Biol Biochem, 1996, 28: 1061-1066.

[19] Six J., Bossuyt H., Degryse S., Denef K. A history of research on the link between (micro) aggregates, soil biota, and soil organic matter dynamics Soil Till Res, 2004, 79: 7-31.

[20] Sjursen H., Michelsen A., Holmstrup M. Effects of freeze-thaw cycles on microarthropods and nutrient availability in a sub-arctic soil Appl Soil Ecol, 2005, 28(1): 79-93.

[21] Smith F.W. The phosphate uptake mechanism Plant Soil, 2002, 245(1): 105-114.

[22] SPSS Inc. SPSS for Windows (11.5), 2002 Chicago, IL: SPSS Inc.

[23] Zeng D., Jiang F., Fan Z., Zu J. Stability of Mongolian pine plantations on sandy soil Chin J Appl Ecol, 1996, 7(4): 337-343.

[24] Zhao Q., Zeng D.H., Lee D.K., He X.Y., Fan Z.P., Jin Y.H. Effects of Pinus sylvestris var. mongolica afforestation on soil phosphorus status of the Keerqin Sandy Lands in China J Arid Environ, 2007, 69(4): 569-582.

[25] Zhenghu D., Honglang X., Zhibao D., Gang W., Drake S. Morphological, physical and chemical properties of aeolian sandy soils in northern China J Arid Environ, 2007, 68(1): 66-76.