Bioassay of Clostera anastomosis granulosis virus

Abstract

The second-instar healthy larvae of Clostera anastomosis were collected in the artificial woodland of poplar in Shuangcheng Town, Heilongjiang Province, China. The dead larvae of C. anastomosis infected by granulosis virus (GV) of Clostera anastomosis were grinded to obtain GV. The GV viral pesticide was diluted to seven concentrations, 1.58×10³PIB·mL⁻¹, 1.58×10⁴ PIB·mL⁻¹, 1.58×10⁵ PIB·mL⁻¹, 1.58×10⁶ PIB·mL⁻¹, 1.58×10⁷ PIB·mL⁻¹, 1.58×10⁸ PIB·mL⁻¹ and 1.58×10⁹ PIB·mL⁻¹ and the fresh poplar leaves were dipped in the seven concentrations liquids to feed the larvae. After nine days the mortality of larvae was investigated. The minimum corrected mortality (7.32%) of larvae was observed at concentrations of 1.58×10³PIB·mL⁻¹ and the maximal mortality (97.36%) was observed at concentration of 1.58×10⁹ PIB·mL⁻¹. The regression equation between the logarithm of the virus concentration and the mortality was y=1.946+0.558x. The LC₅₀ was 2.97×10⁵PIB·mL⁻¹. The LT₅₀ for the virus concentration of 1.58×10⁵, 1.58×10⁶, 1.58×10⁷, 1.58×10⁸, 1.58×10⁹ PIB·mL⁻¹ were 8.55d, 6.89d, 5.9d, 4.65d, and 4.08d, respectively, shorting gradually with the concentration increasing. It is concluded that the toxicity of Clostera anastomosis GV is very strong and as a kind of insecticides it has big potential in practical application.

Keywords

Clostera anastomosis / Larvae / Granulosis virus, Virulence / Bioassay

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